As a new culture mediwn for the Ciliate, Balantidium coili, isolated from a plantar ulcer of a leprosy patient, Sabouraud glucose agar medium(SGA) had several advantages: 1. it was easily made, and 2) the culture grew faster and longer than any previriusly discovered media. Of the contents of SGA, dextrose and peptene are essential nutrient, for the growth of Balantidium coli. When we cultivated Balantidiuum coli. we founund that it produced a nuueoid eubstance and a sweet smilling arcma, forming a colony like tacteria The new straining metlnd for Balantiduin coli. were 3% iodine and carbol-fuchsin stain which atained trophazaite, and cysts more sirnply and distinctly, retaining their original form.
Agar ; Balantidium* ; Ciliophora* ; Foot Ulcer* ; Glucose ; Humans ; Iodine ; Leprosy*

Balantidium coli is the only pathogenic ciliate parasitizing humans. Balantidium coli is widely distributed in hogs, particularly in warm and temperate climates, and in monkeys in the tropics. It is infrequently pathogenic for man. Balantidium coli is considered as a possible pathogen in immunocompromised patients. The infection fundamentally affects the colon and cause variable clinical pictures, from asymptomatic to serious dysenteric forms. Apart from intestinal involvement there have been reports on lung, liver, vagina infection by Balantidium coli. Balantidium coli were observed in the gastric juice of neonate. this is first report of asymptomatic balantidiasis in neonate.
Balantidiasis ; Balantidium ; Climate ; Colon ; Gastric Juice ; Haplorhini ; Humans ; Immunocompromised Host ; Infant, Newborn ; Liver ; Lung ; Vagina

A new trichostome ciliate, Balantidium honghuensis n. sp., was isolated from the recta of Rana nigromaculata and R. limnocharis during parasite surveys in Honghu Lake, Hubei Province, central China in summer of 2010 and 2011. Its detailed morphometric characters based on LM and SEM studies were described herein. The organism is oval in shape and thickly ciliated. The vestibulum is "V" shaped and occupies about 1/3 to 2/5 of the body length. The vestibular and nearby regions possess strong peripheral fibers which form a marked axial fiber about the cytopharynx. More than 10 contractile vacuoles are distributed along the periphery of the latter body. Comparisions were made between this new species and B. sinensis Nie, 1935. They were discriminated from each other in terms of general body forms, body size, and vestibulum shapes. Besides, special attention was paid to its high-speed daughter swarmers which we believed to be the infective stage of B. honghuensis. Possible infection routes of anura amphibian balantidia were discussed.
Animals ; Balantidiasis/parasitology/*veterinary ; Balantidium/classification/*isolation & purification/physiology ; China ; Ranidae/*parasitology ; Rectum/*parasitology

This study was carried out to determine the pathogen-causing diarrhoea in sheep Ovis aries in the Qinghai Tibetan Plateau Area, China. A trophozoite was identified as species of ciliate alveolates infecting the sheep based on morphological characteristics examined by microscope. It was mostly spherical, colourless and transparent, with many vesicles. Macronucleus and contractile vacuoles could not be distinguished. Size of the trophozoite was 80–180×70–150 μm and its surface was covered with cilia. Molecular analysis based on sequences of 18S rRNA and ITS genes confirmed the ciliate species as Balantidium coli. According to the literature, there have been many epidemiological investigations of B. coli infection in pigs, monkeys and humans. To our knowledge, this was the first report of B. coli infections in sheep in the Qinghai Tibetan Plateau Area of China, or eleswhere around the world. Importantly, the sheep case was rare but raised our concern that B. coli may spread across species and expand its host range.
Balantidium ; China ; Cilia ; Haplorhini ; Host Specificity ; Humans ; Macronucleus ; Sheep ; Sheep, Domestic ; Swine ; Trophozoites ; Vacuoles

From the leprosy patients hospitalized at the Korean National Leprosarium on Snrokdo Island, forty-five leprosy patients with plantar ulcers were selected randomly for microbiological studies. A total of 84 strains of bacteria, with the most common being Neieseria sicca (25 strains: 29.8%), 34 strains (40. 5%) were present as a pure growth and 50 strains (59. 5%) were present in ulcers with multiple infection. Antibiotic susceptibility tests indicated that bacterial isolates were rather highly susceptible to gentarnicin and kanamycin, but varying degree of isolates were resistant to 12 antibiotics including streptomycin, rifampicin, lincomycin, penicillin, terramycin and colimycin etc. From a total of 30 plantar ulcers, 17 ulcers produced 24 strains of fungi and 1 strain of Balanticlium coli. They consisted of 17 strains of saprophytic fungi (70. 8%) and 7 strains of yeast-like fungi (29. 2%). Of the culture media for Balantidium coli, Sabourauds glucos medium is the most specific and selective that the author found.
Anti-Bacterial Agents ; Bacteria ; Balantidium ; Colistin ; Culture Media ; Foot Ulcer* ; Fungi ; Humans ; Kanamycin ; Leprosy* ; Lincomycin ; Oxytetracycline ; Penicillins ; Rifampin ; Streptomycin ; Ulcer

Balantidium coli is widely distributed in hogs, particularly in warm and temperate climates, and in monkeys in the tropics. B. coli is the only pathogenic ciliate and is the largest protozoan parasitizing humans. Some individuals with B. coli infections are totally asymptomatic, whereas others have symptoms of severe dysentery similar to those seen in patients with amebiasis. We report a 5-year-old girl with asymptomatic balantidiasis. The patient was suffering from herpes zoster for several days. She did not have symptoms of dysentery or urinary tract infection. Motile trophozoites of B. coli were observed in the urinary sediment. This is the first report of asymptomatic balantidiasis in Korea.
Amebiasis ; Balantidiasis ; Balantidium* ; Child, Preschool ; Climate ; Dysentery ; Female ; Haplorhini ; Herpes Zoster ; Humans ; Korea ; Trophozoites ; Urinary Tract Infections

Annual proficiency surveys were conducted in March, May, and August of 2017 as the Korean Association of External Quality Assessment Service. Overall, four image samples (MPI-17-01, MPI-17-02, MPI-17-03, MPI-17-04) in the first trial, three image samples (MPI-17-05, MPI-17-06 , MPI-17-07) in the second trial, and a slide specimen (MPS-17-01) using parasite samples in the third trial were distributed to participating institutions. The first and second trial specimens were prepared by photographing slides made of formalin-ether concentrate of positive samples stored for educational purposes. The slide distributed in the third trial was prepared using cellophane tape, which was stored after diagnosis of the patients infected with Enterobius vermicularis . There were 191 participating institutions in the first, 204 in the second, and 212 in the third trial. The correct identification rates were 27.2% for MPI-17-01 Diphyllobothrium species (sp.), 96.6% for MPI-17-02 no parasite, 67.5% for MPI-17-03 Metagonimus yokogawai , 71.2% for MPI-17-04 Balantidium coli , 99.0% for MPI-17-05 Taenia sp., 99.0% for MPI-17-06 Trichuris trichiura , 92.7% for MPI-17-07 Cryptosporidium sp., and 96.7% for MPS-17-01 E. vermicularis . The current external quality assessment for clinical parasitology was performed using image samples and standard slides. Surveys of parasitic infections should be accompanied by continuous education on various parasitic infections, for which there was lack of experience of inspection in clinical laboratories. In the future, it will be necessary to establish a standard material using parasitic samples, and ultimately to conduct a survey on whole series of tests for the diagnosis of parasitic diseases.
Balantidium ; Cellophane ; Cryptosporidium ; Diagnosis ; Diphyllobothrium ; Education ; Enterobius ; Heterophyidae ; Humans ; Korea* ; Parasites ; Parasitic Diseases ; Parasitology* ; Quality Control ; Taenia ; Trichuris

Balantidium coli is the only largest ciliated protozoon known to infect human and nonhuman primates. Balantidiasis is a zoonotic disease and is acquired by humans via fecal-oral contact between pigs and humans. The clinical manifestation includes mainly gastrointestinal symptoms; diarrhea and abdominal pain, but in rare cases extraintestinal spread to lungs has been reported. A few reports of B. coli were found in vaginal secretion, skin, gastric juice, and omentum, but there have been no previous isolated cases in the respiratory tract in Korea. We reported that the first case of pneumonia caused by B. coli in Korea in an immunocompetent 40-year-old woman who displayed symptoms of chest discomfort and cough, and was cured with metronidazole.
Abdominal Pain ; Adult ; Balantidiasis ; Balantidium ; Cough ; Diarrhea ; Female ; Gastric Juice ; Humans ; Korea ; Lung ; Metronidazole ; Omentum ; Pneumonia ; Primates ; Respiratory System ; Skin ; Swine ; Thorax

A 4-year-old female Barbary sheep (Ammotragus lervia) was found dead in the Gwangju Uchi Park Zoo. The animal had previously exhibited weakness and lethargy, but no signs of diarrhea. The carcass was emaciated upon presentation. The main gross lesion was characterized by severe serous atrophy of the fat tissues of the coronary and left ventricular grooves, resulting in the transformation of the fat to a gelatinous material. The rumen was fully distended with food, while the abomasum evidenced mucosal corrugation with slight congestion. Microscopic examination revealed the presence of Balantidium coli trophozoites within the lymphatic ducts of the gastric lymph node and the abdominal submucosa. On rare occasions, these organisms may invade extra-intestinal organs, in this case the gastric lymph nodes and abomasum.
Abomasum/parasitology ; Animals ; Atrophy/pathology/veterinary ; Balantidiasis/diagnosis/*veterinary ; Balantidium/*isolation&purification ; Female ; Heart Diseases/pathology/veterinary ; Lymph Nodes/*parasitology ; Sheep ; Sheep Diseases/diagnosis/*parasitology ; Stomach/*immunology/parasitology

Researches in veterinary protozoology until the end of World War II were carried out entirely by Japanese workers such as Danagga, Yamada, Yuhoba and Isshiki. After the Korean war, investigations have been made by several Korean researchers such as Lee's, Son, Joen and Jang. The total species and genera of veterinary protozoa reported by authors up to date were 19 genera and 59 species except the commensal ciliates. Incidence of the protozoa were carried out for 4 years(1972-l975) by examining fecal samples and vaginal swabs in order to know the parasitic status of the domestic animals and poultry. The results are shown as followings. In dairy cattle: Entamoeba bovis 41.4 per cent , Eimeria spp. 5.6-15.0 per cent. In native cattle: Entamoeba bovis 47.6 per cent, Tritrichomonas foetus 8.5 per cent. In native horse: Eimeria sp. 10.0 per cent. In goat: Eimeria spp. 100 per cent. In swine: Entamoeba spp. 55.44 per cent, Eimeria spp. 20 per cent, Balantidium coli 66.58 per cent. In dog: Isospora bigemina(Large type) 8.7 per cent, Giardia canis 4.4 per cent. In cat: Isospora spp. 12.8 per cent. In hare: Entamoeba cuniculi 12.0 per cent, Eimeria spp. 20.0 per cent, Chilomastix cuniculi 12.0 per cent , Giardia cuniculi 4.0 per cent. In turkey: Eimeria spp. 33.3 per cent, Hexamita meleagriais 8.3 per cent. In chicken: Eimeria spp. 26.8 per cent. The main protozoan diseases of the domestic animals and poultry in Korea may be summarized from the data reported and surveyed as followings. Coccidiosis; poultry, hare, goat, cattle, swine. Theileriasis; cattle. Babesiasis; cattle, dog. Anaplasmosis; cattle, goat. Eperythrozoonosis; cattle, swine. Leucocytozoonosis; chicken. Histomoniasis; chicken. Toxoplasmosis; swine, cat. Balantidiasis; swine. Besnoitiosis; native cattle. Amoebiasis; swine, cattle. Sarcosporidiosis; Cattle, swine.
parasitology-protozoa ; epidemiology ; cattle ; horse ; swine ; dog ; hare ; turkey ; cat ; Entamoeba bovis ; Eimeria spp. ; tritrichomonas foetus ; Entamoeba spp. ; Balantidium coli ; Isospora bigemina ; Giardia canis ; Isospora spp. ; Entamoeba cuniculi ; Chilomastix cuniculi ; Giardia cuniculi ; Hexamita meleagriais ; Coccidiosis ; Theileriasis ; Babesiasis ; Anaplasmosis ; Eperythrozoonosis ; Leucocytozoonosis ; Histomoniasis ; Toxoplasmosis ; Balantidiasis ; Besnoitiosis ; Amoebiasis ; Sarcosporidiosis