A follow-up observation of 222 HBsAg carriers was carried out over four years. The serological results showed that 46 out of 222 HBsAg carriers were negative converted (20.7%) , 25 out of 72 HBeAg positive carriers were negative converted (34.72%). The clinical results showed that 12 suffered from acute hepatitis, 10 from chronic persistent hepatitis (CPH), 8 chronic active hepatitis(CAH), 3 hepa-tocirrhosis, 1 primary hepatic carcinoma (PHC), 25 with slight liver function damage, 117 were asymptomatic carriers, and 46 persons recovered.This follow-up study indicated that the negative-conversion rate of HBsAg carriers with high titer or HBeAg positive was significantly lower than those with low titer or HBeAg negative and that the negative-conversion rate of HBsAg carriers or HBeAg positive increased with age