Objective: In order to establish an effectively analytic method about the content of lead, arsenic, cadmium, mercury and copper. Methods:According to the standard addition method, matrix modifiers (nickle nitrate and diammonium orthphosphate) were added to sample and the contents of Pb, As, Cd and Cu were determined by graphite furnace atomic absorption spectrometry, reduce agent (acid tin bichloride was added to sample and the content of Hg was determined by cold vapour atomic absorption method. Results: The average recoveries of lead arsenic, cadmium, copper and mercury were Pb=97.5%, RSD = 2.75%; As=96.6%, RSD = 2.23%; Cd= 98.2% , RSD = 1.32% ;Cu=97.8%, RSD =1.19%;Hg= 98.8% , RSD = 1.85 , respectively. Conclusion: The method is sensible, accurate and reproducible. and applied for quality control of content of heavy metals in Qijidehuang Pills.

Recently,immunologists have payed the attention to the effects of ionizing radiation on tumor immunity,and attempted to induce and improve anti-tumor immune effects with it.More and more evidences showed that exact radiotherapy scheme and irradiation dose,particularly combined with immunotherapy,could induce or regulate systemic immune response and contributed to tumor control and inflammatory occurrence.This paper reviewed the effects and mechanisms of radiotherapy on tumor immune and the results in combination with immunotherapy here for guiding the effective combined application of radiotherapy and immunotherapy.

Objective To investigate the application prospective of carboxyfullerene C_3 as a radioprotectant or assistant for tumor radiotherapy.Methods Different concentrations of C_3 were incubated with K562 and AHH-1 cell,CCK-8 assay and trypan blue rejection test were performed to examine the influence of C_3 on the cell viability.Annexin V/PI staining and flow cytometry assay were applied to assess the cell cycle and apoptosis after 7-ray irradiation.Results C_3 showed little toxicity to AHH-1 cell with the survival rate over 95% ,but 600 mg/L of C_3 markedly inhibited the growth of K562 cell (82%) .Pretreatment of 100 mg/L C_3 significantly increased the survival rate of AHH-1 cell after 4 Gy irradiation compared with the single radiation group(71.3% vs 90.3%) ,but decreased the apoptosis rate (26.3% vs 12.6%) ,while the survival rate of K562 cell was decreased and the apoptosis rate was elevated with the increase of C_3 concentration.Moreover,the cell cycle analysis revealed the G_2 phase block in AHH-1 cell after radiation exposure was mitigated by C_3 pretreatment,but that in K562 cell was aggravated.Conclusions C_3 has good radioprotective effects on AHH-1 cells.For K562 cell,C_3 could inhibit the cell proliferation,promote the radiation induced apoptosis and aggravate the G_2 phase block.

Surgery,radiotherapy and chemotherapy are three traditional treatments for malignant tumors.With the development of medicine,immunotherapy has been gradually adopted as an emerging therapy of malignancies.Recent clinical studies have demonstrated that the combination of radiotherapy and immunotherapy can induce the abscopal effect and improve the prognosis of patients.Compared with the conventional radiotherapy,stereotactic radiotherapy has a larger single dose and higher accuracy,which is more likely to induce the bystander effect and anti-tumor response.The combination of stereotactic radiotherapy and immunotherapy has been proven to be a more promising therapy in certain clinical trials.However,not all types of tumors can benefit from such combined therapy in clinical practice.The optimal dose,fraction pattern and lesion of radiotherapy,immune enhancement and safety remain to be further clarified.In this article,the research progress,related controversies and future research direction of stereotactic radiotherapy combined with immunotherapy for malignancies were reviewed.

Objective To investigate the perioperative complications and therapeutic effects of balloon-assisted coiling (BAC) and stent-assisted coiling (SAC) in patients with ruptured intracranial aneurysms in the acute phase. Methods Totally 91 patients with 91 intracranial ruptured aneurysms were treated with BAC or SAC in our hospital between January 2014 and December 2016. Among them, 37 patients were treated with BAC and 54 patients with SAC respectively. Of the two groups, the position distribution and shape of aneurysms, and the complications after procedures and the therapeutic effects were summarized and evaluated retrospectively using chi-square test. Results The width of the aneurysm neck was narrower in the BAC-treated group compared to the SAC-treated group (3.31±1.63 mm vs. 4.35±2.10 mm, P=0.01). The aneurysm body/neck ratio (B/N) was lower in the BAC-treated group than in the SAC-treated group (1.64 ± 0.46 vs. 1.35±0.66, P=0.025). The recurrence rate was higher in the BAC-treated group than that in the SAC-treated group (18.9％ vs. 0.9％, P=0.005). There was no statistical difference in perioperative complication in both the BAC-treated group and SAC-treated group. However, 2 patients died due to the relative postoperative intracranial bleeding in the SAC-treated group. Better outcomes (Modified Rankin Score, mRS, 0-2) were achieved in the BAC-treated group compared to the SAC-treated group (94.6％ vs. 88.9％, P=0.028) at the follow-up visit. Conclusions These findings suggested that there is no difference between the BAC-treated group and the SAC-treated group in the risk of complication. BAC can achieve a better prognosis,but it is more prone to relapse. The SAC method was more appropriate for wider neck aneurysms. It was also an option to coiling the aneurysm in BAC in acute phase firstly, followed by additional treatment in SAC during the follow-up period.

Objective To investigate the effect of Yes-associated protein(YAP)on intervertebral disc nucleus pulposus cells and its possible mechanism.Methods The relatively normal and degenerative intervertebral disc tissues of patients who underwent lumbar surgery in our hospital from March 2021 to July 2022 were harvested,and then the expression of YAP in the tissues were detected by immunohistochemistry assay and Western blotting.Human primary nucleus pulposus cells were isolated and primarily cultured,and treated with IL-1β to induce degeneration.Then the cells was divided into control group,IL-1β group,IL-1β+LV-YAP group,IL-1β+YAP-siRNA group,and IL-1β+LV-YAP+3-MA group.Western blot analysis was used to detect the expression of the proteins related to extracellular matrix catabolism and autophagy in each group.Finally,a rat model of disc degeneration was established,and the expression of YAP and LC3 and disc degeneration were observed with MRI,Alcian blue staining and immunohistochemistry.Results The expression level of YAP was significantly lower in the degraded disc tissues than the relatively normal disc tissues(P＜0.05).The IL-1β+LV-YAP group had significantly increased protein levels of Collagen Ⅱ,Aggrecan,and LC3-11(P＜0.05),and decreased levels of MMP-3 and MMP-13(P＜0.05)when compared with the cells after IL-1β treatment,whereas the IL-1β+YAP-siRNA group showed the exact opposite effects.What's more,pretreatment with autophagy inhibitor 3-MA resulted in decreased number of GFP-LC3 positive particles and protein levels of Collagen Ⅱ,Aggrecan and LC3-Ⅱ(P＜0.05),and increased protein expression of MMP-3 and MMP-13(P＜0.05)in comparison with the conditions in the IL-1β+LV-YAP group.Furthermore,YAP overexpression promoted LC3 expression and inhibited disc degeneration in rat model of disc degeneration.Conclusion YAP overexpression can inhibit extracellular matrix degradation by promoting autophagy in human nucleus pulposus cells and thus delaying disc degeneration.

There are differences and inconsistencies to some degree in the radioactive contamination control level of personnel′s body surface availiable in many national standards, thus puzzling the users. Therefore, it is proposed to compare the applicable scope, conditions and differences between relevant national standards, and combine with similar clinical nuclear medicine standards of radiological protection content to presevent recommendations on the contamination control level that should be correctly applied in an event of nuclear and radiological emergency. Based on the discussion of similar standards, the contaminated personnel with α of 0.04-10 Bq/cm 2 and β of 0.4-100 Bq/cm 2 are advised to be treated in the institutions with higher than secondary medical insititution. Both α econtamination control levels less than 0.04 Bq/cm 2 and β levels less than 0.4 Bq/cm 2 could be achivable, if fully decontaminated.