Objective To explore the effect of atorvastatin in the treatment of nonalcoholic fatty liver disease and its clinical application value.Methods 110 patients with nonalcoholic fatty liver disease were randomly divided into observation group and control group,55 cases in each group.The control group was given compound dichloroace-tate diisopropylamine orally,the observation group was given atorvastatin therapy.The effects of the two groups were recorded.Results In the observation group after treatment,the alanine aminotransferase was (39.78 ±3.45) U/L, aspartate aminotransferase was (29.17 ±3.17) U/L,gamma glutamine transpeptidase was (54.28 ±4.11) U/L.In the control group after treatment,the alanine aminotransferase was (52.78 ±6.81) U/L,aspartate aminotransferase was (39.96 ±6.21)U/L,gamma glutamine transpeptidase was (68.69 ±8.31)U/L,there were statistically signifi-cant differences between the two groups(t=12.6290,11.4770,11.5273,all P<0.05).In the observation group after treatment,the triglycerides was (1.66 ±0.32)mmol/L,total cholesterol was (3.27 ±0.37)mmol/L,low density lipo-protein was (1.94 ±0.45)mmol/L.In the control group after treatment,the triglycerides was (2.38 ±0.92)mmol/L,total cholesterol was (5.74 ±1.49)mmol/L,low density lipoprotein was (3.46 ±1.17)mmol/L,there were statis-tically significant differences between the two groups(t=5.4818,11.9316,8.9925,all P<0.05).In the observation group after treatment,the ultrasonic score was (1.33 ±0.12),liver/spleen CT ratio was (0.33 ±0.08).In the con-trol group after treatment,the ultrasonic score was (1.78 ±0.35),liver/spleen CT ratio was (0.47 ±0.21),there were statistically significant differences between the two groups(t =9.0197,4.6202,all P <0.05).Conclusion Atorvastatin in the treatment of nonalcoholic fatty liver disease can effectively improve the liver function,reduce blood lipid concentration,minor adverse reactions,and it is worth popularizing in clinical use.

Objective To evaluate a kind of operative management in the treatment of chronic non bacterial prostatitis. Methods From 1992 to 1999,cross incision of the prostatic capsule and bladder neck plasty were performed for 102 cases of chronic non bacterial prostatitis. Results The effective rate is 85.3% (87/102).It was 92.6% for the prostatitis of obstructive type (63/68) and 70.6% for patients complained of pain (24/34).43 cases have been followed up for an average of 4.5 years of which the outcome has been satisfactory in 36 and noneffective in 7. Conclusions The operative management for chronic non bacterial prostatitis is effective,safe,with less damage and less complication.

Objective To assess the urinary continence at a follow-up of 1 year after radical retro-pubic prostatectomy. Methods By using subjective (questionnaire) and objective (pad-test) methods, 165 consecutive prostate cancer patients were followed up at least 1 year. With the purpose to improve post-operative continence, surgical techniques such as preservation of external striated sphincter, preservation of bladder neck and pre-prostate sphincter (PPS), and nerve-sparing technique have been carried out.Analyses were also made in comparison by age. Results A post-operative incontinence rate of 0.7% was found in the sphincter preserving group and 5.3% in the sphincter repairing group. Patients aged 50～60 revealed a continence rate of 60% when the urethral catheter was removed, whereas 82% in the 60～70 group.After 3 months of follow-up, the continence rate of both age groups tended to be similar. Conclusions Surgical improvement and patients' age are important factors in post-operative continence after radical retro-pubic prostatectomy. The preservation of external urethral sphincter helps improve continence rate. Younger patients tend to regain to continence in a shorter period while the elders longer.

Objective To investigate the effect of low intensity microwave electromagnetic fields (EMF) on energy metabolism of cerebral cortical neurons of postnatal rats. Methods The cultured neurons were exposed to low intensity 900 MHz continuous microwave EMF (SAR=0.38-3.22 mW/g) 2 hours a day for 4 to 6 consecutive days. The activity of cytochrome oxidase(CCO) was taken as the index for energy metabolism level. Results The results of cytochemistry showed that compared to the sham-exposed, a significant decrease of cytochrome oxidase activity in exposed neurons(0.38-3.22 mW/g P

Objective To study the expression of MAGE-3 in colorectal cancer (CRC) with different differentiation grades. Method Immunohistochemical technique for MAGE-3 antigen was performed in paraffin embedded sections of 101 CRC specimens. The relationship between differentiation and Dukes classification of CRC and the expression of MAGE-3 antigen was analyzed. Result MAGE-3 was only expressed in tumor tissues with the total positive rate of 31.7%. The rate and intensity of MAGE-3 in poorly differentiated group were higher than that of the well differentiated group (P0.05). Conclusion MAGE-3 may be a specific molecular differentiation related marker of CRC. Its antigen may have potential use as a new peptide vaccine for immunotherapy for CRC.

Asthma is a chronic respiratory disease with various etiologic factors.Asthma is believed as a result of interaction between gene variation and environmental factors.Asthma increased rapidly worldwide in recent 40 years, air pollutants are believed to be the important triggers, crossover design and multi-pollutant models were used in the further studies.Although some efforts had been made in the mechanism of asthma triggers, they were limited to the lab studies.Studies in asthma related SNP and its interaction with air pollutants will be a revised method in mechanism researches.Based on epidemiological studies, in vivo and in vitro studies, the biological effects and mechanisms of air pollutants on asthma were discussed and evaluated in this paper.

Objective To establish a UV spectrophotometry method and an HPLC method respectively for the determination of the total content of coumarin and contents of four main constituents of coumarin in Fraxini Cortex extract.Methods UV spectrophotometry was used for the determination of the content of total coumarin in Fraxini Cortex extract. The reference substance was Aesculin, and the maximum ultraviolet absorption wavelength was 334 nm. The HPLC method was used to determine the contents of Aesculin, Fraxin, Aesculetin and Fraxetin in Fraxini Cortex extract, using gradient elution with acetonitrile-phosphate solution (0.01%) as mobile phase on Agilent ZORBAX SB-C18 chromatographic column (4.6 mm × 250 mm, 5μm) at room temperature.Results For the UV method, the linear range of the mass concentration of Aesculin was 5.76-23.04μg/mL (r=0.999 9), and the average recovery was 100.6% (RSD=1.8%). For the HPLC method, the linear ranges of the mass of Aesculin, Fraxin, Aesculetin and Fraxetin were 0.055 0-3.850 0μg (r=0.9997), 0.053 9-3.773 0μg (r=0.999 8), 0.060 0-0.660 0μg (r=0.999 9), and 0.056 2-0.618 2μg (r=0.999 9), respectively, and the average recoveries were 96.97% (RSD=1.26%), 100.80% (RSD=2.22%), 99.04% (RSD=2.47%), and 98.77% (RSD=1.94%), respectively.Conclusion Both of the two methods are simple, accurate and reliable, and can be used for the quality control of total coumarin and the main constituents of coumarin in Fraxini Cortex extract.

Humans ; Medicine, Chinese Traditional ; methods ; Tongue ; metabolism ; pathology

Objective To investigate the predictive value of dose?volume histograms ( DVHs ) of organs at risk ( OARs ) including the bladder, rectum, and small intestine in volumetric modulated arc therapy ( VMAT) plans for cervical cancer. Methods A total of 100 VMAT plans for cervical cancer were assigned into the learning group. The correlation of the anatomical information with the V30 , V40 , and V50 values of the bladder, rectum, and small intestine was evaluated in the group. The support vector regression ( SVR) algorithm was used to establish the correspondence between the anatomical information and the DVHs of OARs. The DVHs of OARs in the verification group containing 20 VMAT plans were predicted based on the anatomical information. Results The DVHs of the bladder, rectum, and small intestine were likely to be influenced mainly by the spatial relationship between these OARs and target volume. In the verification group, the prediction errors of V30,V40 and V50 by SVR algorithm were-2.4%±3. 5%,-2.5%±3. 8%, and-1.5%±4. 9% for the bladder, 0.5%±2. 6%,-1.5%±5. 1%, and-2.0%±7. 4% for the rectum, and-2.9%± 5. 3%, 2.7%±7. 7%, and 5.3%±11. 1% for the small intestine, respectively. Conclusions After learning the correlation between the anatomical information and the DVHs of OARs from prior VMAT plans for cervical cancer, SVR algorithm can precisely predict the DVHs of the bladder, rectum, and small intestine based on the anatomical information.

Objective To study the procedure of imaging examination for the patients with colon cancer.Methods The comparative study on ultrosonograph, pneumobarium double contrast examination, colonscopy and CT in 82 cases with colon carcinoma comfired by operation and pathalogy were carried out.Results The detective rate of colon cancer was 90.1%,89.5%,53.8% and 53.3% by pneumobarium double contrast examination,colonscopy ultrosonography and CT respectively.The detective rate of metastasis in liver was 100% by ultrosonograph and CT.Conclusion Pneumobarium double contrast examination and colonoscopy are the main methods for diagnosis of colon cancer. Ultrosonography and CT are the important methods in evaluating the stage of colon carcinoma before operation.