AIM: To identify the changes in microRNA ( miRNA ) profile of human lens epithelium ( HLE) induced by H2 O2 and the role of miRNA in oxidative stress induced apoptosis.
METHODS: HLE cell line HLE - B3 was treated by 100μmol/L H2 O2 for 24h and the total RNA were isolated by Trizol reagent. miRNA profile was generated by miRCURYTM LNA microRNA Array. The target genes of differentially expressed miRNAs were predicted by bioinformatics software.
RESULTS:Twenty-eight miRNAs showed significantly differential expression after H2 O2 treatment, 18 miRNAs upregulated and 10 miRNAs downregulated. The differentially expressed miRNAs may involve in apoptosis of lens epithetium and development of cataract through targeting BCL2L2 and MIP.
CONCLUSION: H2 O2 can induce dramatically changes in miRNA profile of HLE, which may play a pivotal role in the pathogenesis and development of cataract.

Objective To study the serum lipid(TC,TG,LDL-C) and the role of typeⅠcollagen,NF-?B,typeⅠcollagen mRNA and NF-?B mRNA levels and their mechanisms in the occurrence and progress of atherosclerosis in order to provide theoretical gist for its prevention.Methods Blood vessel wall pathological and the serum lipid changes,the expressions of typeⅠcollagen,NF-?B,typeⅠcollagen mRNA and NF-?B mRNA levels were observed in dietary induced atherosclerosis rabbit model by morphology study,immunohistochemistry,in situ hybridization technique and color image analyzer(CMAIS).Results The model of AS was established successfully after rabbits were fed with cholesterol,who developed the fatty streak lesion in the aortic intra-wall.In the rabbit model,TC and LDL were respectively increased 43 times and 37 times and there were no difference in TG and body weight.Type I collagen,NF-?B,type I collagen mRNA and NF-?B mRNA were 0.27?0.02,0.19?(0.01) and 0.30?0.03,0.35?0.03,respectively,which were higher than those of control group(0.08?0.01,0.09?0.01 and 0.11?0.01,0.10?0.09,respectively)(P

Objective:To investigate the preparation of paclitaxel-loaded nanoparticles targeting liver cancer stem cells and their effects on liver cancer HepG2 cells (CD133 positive subset accounting for 8%) and Huh-7 cells (CD133 positive subset accounting for 65%).Methods:Poly (lactic-co-glycolic acid)-loaded paclitaxel nanoparticles were prepared by using emulsification-solvent evaporation method. Paclitaxel-loaded nanoparticles decorated with anti-CD133 antibody, called targeted nanoparticles, were prepared by using 1- (3-dimethylaminopropyl)-3-ethylcarbodiimide/N-hydroxysuccinimide (EDC/NHS) cross-linking method. The manifestations and physicochemical characteristics of the nanoparticles including encapsulation efficiency, loading efficiency, particle size distribution, morphology and release in vitro were studied. Liver cancer Huh-7 and HepG2 cells accompanying with paclitaxel-loaded nanoparticles or targeted nanoparticles were cocultured. The uptake and accumulation of nanoparticles by liver cancer cells were analyzed by using flow cytometry, and positive cell proportion of CD133 was also detected. Cell survival was analyzed by using plate clonogenesis assay.Results:Scan electromicroscopy result showed particle size of targeted nanoparticles was (429.26±41.53) nm with zeta potential of -11.2 mV; targeted nanoparticles were possessed with spherical morphology and higher encapsulation efficiency [(87.53±5.90) %]. Flow cytometry showed that in Huh-7 cells at 37℃, the fluorescence intensity of targeted nanoparticles group (13 397±720) was higher than that of paclitaxel-loaded nanoparticles group (6 898±604), and the difference was statistically significant ( P < 0.05); there was no statistically difference in the fluorescence intensity of HepG2 cells in paclitaxel-loaded nanoparticles group and targeted nanoparticles group at 37 ℃ (7 899±343 vs. 8 432±516, P>0.05). CD133 positive cell proportion of Huh-7 cells in targeted nanoparticles group [(15.7±2.6)%] was lower than that in paclitaxel-loaded nanoparticles group [(54.9±7.4)%], and the difference was statistically significant ( t = 7.31, P = 0.008); there was no statistical difference of HepG2 cells between the two goups ( P > 0.05). Plate clonogenesis assay showed that the cell survival rate of Huh-7 cells in targeted nanoparticles group was lower than that in paclitaxel-loaded nanoparticles group at different time points, and the difference was statistically significant ( F = 5.56, P = 0.009); but there was no statistically significant difference in HepG2 cell survival rate between the two groups ( F = 1. 19, P = 0.142). Conclusion:Prepared nanoparticles targeting liver cancer stem cells have a good inhibitory effect on liver cancer Huh-7 cells.

Objective To investigate the organophosphorus pesticide residues in vegetables and fruits in Shnxi area so as to take preventive measures to minimize food contamination. Methods A total of 140 samples of vegetables and fruits were obtained randomly from the local market in Shnxi area. Eight organophosphorus pesticides were analyzed by gas-chromatographic method. Results The incidence of organophosphorus pesticide residues in vegetables and fruits were 20% and 1.7%, respectively. The mean level of parathion in vegetables and dimethoate in fruits were 6.22?g/kg and 3.02?g/kg, exceeding their MRLs. Conclusion There is a misuse of organophosphorus pesticides in Shnxi area and routine monitoring is necessary to reduce food contamination before entering market.

BACKGROUND:Alterations in secondary hemodynamics around the stent edge can be caused by stent planted. Is there different effect of shear stress on platelet growth factors-A, B (PDGF-A, B) and basic fibroblast growth factor (bFGF) of endothelial cells around stent edge after stent planted?OBJECTIVE: To explore the effect of shear stress on growth factors of endothelial cells around stent edge.DESIGN: Observational comparative study.SETTING: Department of Cardiology, Yijishan Hospital, Wannan Medical College; Laboratory of Biomechanics (National Laboratory), Medical College of Shanghai Jiao Tong University.MATERIALS: The experiment was carried out in the Laboratory of Biomechanics, Medical College of Shanghai Jiaotong University from April to October 2006. The main reagents were detailed as follows: trypsin (Hyclon Company); M-199 medium (Gibco BRL Company); fetal bovine serum (Hangzhou Sijiqing Bioengineering Material Institute); aFGF, Heparin and Hepes (Sigma Company); thymide, Ⅷ factor monoclonal antibody (Rabbit anti-human),biotinylated horse anti-rabbit IgG and propidium lodide (Sigma Company); neonate belly band (Delivery Room of Shanghai the Fifth People's Hospital); trizol (Invitrogene Company); reverse transcription polymerase chain reaction (RT-PCR) kit (Fermentas Company); penicillin/streptomycin (AB/M), objective gene primer (Shanghai Bioengineering Company).METHODS: Traditional parallel-plate streaming cavity was modified into rectangular and gradient shear stress models;while static group was established at the same time. Two shear stress groups were subjected to 11.37 dyne/cm2 and 5.66-14.38 dyne/cm2 shear stress, respectively for 3 hours, 6 hours and 12 hours.MAIN OUTCOME MEASURES: The expressions of PDGF-A, B and bFGF mRNA of human umbilical endothelial cells (HUVECs) were measured by RT-PCR at different time points.RESULTS: Compared with static group, the maximal expression of PDGF-A, B and bFGF mRNA was reached peak at 3 hours in gradient shear stress group and this level of PDGF-A mRNA expression remained till 12 hours. in rectangle shear stress group, the peak value of mRNA expression of these growth factors was all reached at 6 hours, and then gradually decreased.CONCLUSION: Over-expressions of PDGF-A, B and bFGF induced by gradientshear stress after stent planted may play an important role in stent restenosis through intimal hyperplasia.

Objective The aim of the present study was to investigate the ability to perceive syllables phrases ,mono-syllabic words ,disyllabic words ,when using a CI and an HA in non -implanted ears (bimodal fitting) ,and the relationship be-tween aided thresholds in the HA ear and bimodal effectiveness .Methods Eighteen children who consistently used a bimodal fitting participated in the study .The loudness of speech in the HA ear matched that with implanted ear ,and the aided thresh-olds in the HA ear were obtained .The recognition rate of syllables phrases ,monosyllabic words ,and disyllabic words was tested under the aforementioned two modes in both the quiet and noisy backgrounds .Results The speech performance of children for monosyllabic words were 82 .67% ± 12 .23% ,83 .61% ± 12 .22% ,for disyllabic words 76 .00% ± 16 .13% ,78 .11% ± 14 .84% , for syllables phrases 60 .11% ± 17 .18% ,65 .43% ± 16 .76% ,with a CI alone or with bimodal fitting in a quiet environment ,re-spectively .In a noisy environment ,monosyllabic words were 75 .50% ± 14 .12% ,76 .83% ± 14 .15% ,and disyllabic words were 68 .22% ± 17 .15% ,77 .18% ± 16 .83% ,and syllables phrases were 49 .39% ± 19 .26% ,56 .33% ± 19 .55% .The results sug-gested that speech performance in a quiet or a noisy environment was significantly better with bimodal hearing than with a CI a-lone .All were statistically significant except the recognition rate of monosyllabic words in quiet background (P<0 .05) .Signifi-cant negative correlations were found between aided thresholds at 250 and 500 Hz ,and the bimodal advantages were noticeable for the some speeching perception .Conclusion Bimodal hearing enhances speech performance for deaf patients .The low -fre-quency residual hearing in the HA ear may play a major role in enhancing auditory and speech performance .

0.05),that there was negative expression of PP2Ac protein in the cytoplasm of the human lung squamous carcer and small cell lung cancer while positive expression of PP2Ac protein in intercellular substance,and that there were negtive expression of PP2A in human lung adencarcinoma and overexpression of p-AKT protein in human lung adencarcinoma.Western blot technique analysis showed that there was nomal expression of PP2A protein in normal human lung tissue,and lower expression in YTLMC-90 cell than in nomal human lung tissue.While sharp low expression GLC-82 cell.Adding okadaic acid(OA),an inhibitor of PP2A,the expressiong of p-AKT in GLC-82 cells was sharply higher than in normal lung tissue at 6 h,12 h and 24 h,indicating that inverse correlation between PP2A and p-AKT protein expression.Conclusions PP2A is involved in human lung carcinomar,its expression level is related to histological type of human lung carcer.Activition of AKT(p-AKT)is related tightly to human lung carcer.

Objective To evaluate the effect of capsular tension ring (CTR) in phacoemulsification for the patients with subluxated lens extraction. Methods 15 patients (16 eyes) with subluxated ca tractous lenses were managed by implanting CTR after phacoemulsification and implantation of posterior chamber intraocular lens. Results Thirteen eyes with IOL were basically in the normal position, 3 with lightly tilting at 3 month after the operation. Postoperative visualacuity were all satisfitied. No complication was found caused by CTR. Conclusion It' s an effective way to perform phacoemulsification and CTR for cataract patients with subluxated lens. This method was simple with high security and good to the recovery of visual function in the early stage after the operation.

Objective To investigate the effect of immunocompetent cells and immune regulator on the apoptosis of human mesenchymal stem cells ( MSCs) and on mRNA expression of heme oxygenase-1. Methods MSCs were cultured by density gradient centrifugation and then identified by flow cytometry. RT-PCR was used to detect HO-1 mRNA expression and flow cytometry was used to analyze cell apoptosis after the stimulation of IFN-? and PHA-activated T cells. Results The mRNA expression of Heme oxygenase-1 was observed in MSCs and decreased after the stimulation of IFN-? and activated T cells. IFN-?,znpp-Ⅸ and combined these two caused obvious cell apoptosis in MSCs,with an apoptotic rate of ( 56. 50 ? 0. 16) % ,( 56. 85 ? 2. 27) % ,and ( 82. 53 ? 2. 65) % respectively. All of them had a significant difference compared with the normal MSCs [( 7. 56 ? 1. 43) % ,P

Objective To determine the expression of cancer stem cell markers, CD133, CD44 and OCT-4, in small cell lung cancer cell line NCI-H82 and confirm the specific markers. Methods NCI-H82 cells were cultured, and the expressions of markers (CD133, CD44 and OCT-4) were detected by immunofluorescence staining. The immunohistochemistry was performed to detect the expressions of CD133, CD44 and OCT-4 in 79 tissues with small cell lung cancer. Results In NCI-H82 cells, the florescence was positive in CD133 and CD44 and negative in OCT-4. In the tissues, the expression of CD133 and CD 44 was related with tumor size, lymph node metastasis and clinical stage (P< 0.05), while OCT-4 was negative expression. Conclusion CD133 and CD44 might be the cancer stem cell markers of small cell lung cancer, which maybe have clinical significance on diagnosis and treatment.