Objective To explore the nurses'self-efficacy for palliative care and its related factors.Methods Data were collected via a self-constructed questionnaire,using the purposive sampling method.Subjects were nurses from two “grade-A” general hospitals in Henan province.Results The nurses' self-efficacy for palliative care stayed at a disequilibrium state,mean score of self-efficacy for physical care was 4.00,followed by family care self-efficacy 3.85,then psychological and spiritual care self-efficacy 3.70.Significant difference existed in self-efficacy for palliative care in nurses having different attitudes toward death.The cognitive level for palliative care,past experience of caring for end-stage patients were positively correlated with self-efficacy.Conclusions The main factors of the nurses' self-efficacy for palliative care related to attitudes toward death,past experience of caring for end-stage patients and the cognition level of nurses to palliative care.

Objective To observe the effect of Mongolian medicine Tonglaga-5 Pill on the osteoporosis induced by retinoic acid in the rats,and to provide experminted basis for its clinical application.Methods 60 female Wistar rats were randomly divided into blank group,model group,Ossptide Tablets group,Tonglaga-5 Pill groups with low,medium and high doses (n=10).The osteoporosis models were induced by retinoic acid in the rats.The levels of serum calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), transforming growth factorβ1 (TGF-β1),insulin like growth factor (IGF-1)and bone indexes were detected and the contents of Ca and P in ash were also detected,and the changes of bone histomorphometry of the rats were observed under light microscope by HE staining.Results Compared with model group,the serum calcium and P contents of the rats in Tonglaga-5 Pill groups were increased,but there were no significant differences (P>0.05);the ALP levels were decreased significantly (P<0.05);the TGF-β1 and IGF-1 had no significant changes (P>0.05);the contents of calcium and P in ash were increased in different degrees,but there were no significant differences (P>0.05).Compared with control group,the femur maximum width index and minmum width index of the rats in model group were significantly decreased (P<0.05 or P<0.01);the femur length index was increased (P<0.05).Compared with model group,the femur maximum width index and minmum width index of the rats in Tonglaga-5 Pill groups had no changes (P>0.05).Under light microscope,the rich and full bone trabecula was found in the rat femur in control group;the bone trabecula in model group was decreased,and the bone trabecula in Tonglaga-5 Pill groups was increased. Compared with model group, the trabecular bone areas in Tonglage-5 pill groups and Ossotide group were increased (P<0.05).Conclusion Tonglaga-5 Pill displays a certain preventive effect on the rats with retinoic acid-induced osteoporosis,which can blocks the bone mineral loss,bone tissue structure changes and bone quality decreasing effectively.

Objective:To analyze the pulpal prognosis of replanted permanent teeth in children and to examine the associated factors .Methods:The samples consisted of 67 children with 81 avulsed perma-nent teeth treated in Peking University Hospital of Stomatology from 2000 to 2012 .Their clinical data of the replanted teeth were collected , and the follow-up period was no less than 12 months.The pulpal prognosis was classified as pulp necrosis and pulp non-necrosis .Results: The replantation within 30 minutes after avulsion led to the most significant increase in pulpal healing (P<0.05).The dental pulp of 80% ( 4/5 ) young permanent teeth replanted within 30 minutes remained vital , while all the teeth replanted after 30 minutes developed pulp necrosis within 60 days after replantation .Conclusion: The extra-alveolar period significantly affects the prognosis of pulp in immature permanent teeth after replanta-tion.When the extra-alveolar period is more than 30 minutes, the chance of pulp revascularization after replantation is very low , therefore pulp extirpation should be performed .

Objective To investigate the effect of bifidobacterium (Bif) supplementation on acute inflammatory response in murine dextran sulfate sodium(DSS)-induced colitis, and its possible mechanism. Methods Mice were divided into four groups: control,DSS, salfasalazine (SASP) and Bif. The mice in groups DSS, SASP and Bif were fed with 5% DSS(w/v) solution for 7 days to induce colitis, and disease activity index (DAI) was calculated every day. The mice in group SASP were fed with SASP every day during inducing colitis, and the mice in Bif group were given Bif by oral gavage from 7 days before the experiment to the end of experiment. The expression of TNF-?、NF-?B P65 and myeloperoxidase (MPO) in inflamed colon of each group was measured at the end of experiment. Results The mice in groups SASP and Bif showed a lower DAI than those in group DSS since the forth day to the end of experiment. There were lower level of TNF-? and MPO in murine inflammatory colon, and lower NF-?B P65 expression in nuclei of inflammatory cells in groups SASP and Bif than those in control at the end of experiment. Conclusions Treatment with Bif can effectively inhibit proinflammatory cytokine secretion and NF-?B activation in inflammatory cells, and decrease colonic inflammatory response in DSS induced colitis.

Objective:To analyze the prognosis about periodontal ligament healing of replanted perma-nent teeth in children and to examine the associated factors.Methods: The sample consisted of 49 children with 61 avulsed permanent teeth, whose injuries had been managed in the period from 2000 to 2012.The clinical data of replanted teeth were collected, and the follow-up period was no less than 12 months .The factors were analyzed in relation to postoperative outcomes, classified as functional periodon-tal healing ( FH ) , infection-related ( inflammatory ) resorption ( IRR ) and replacement resorption ( RR) .Results:The functional healing rate was 23.0%, while replacement resorption rate was 72.1%. The replacement resorption ( ankylosis ) was usually observed earlier by clinical examination than by radiographic examination.86.0% (40/47) resorptive processes were diagnosed within the first year. Physiological storages, such as milk, saline and saliva were significantly better to periodontal ligament healing than nonphysiological storages, such as tap water and sterilizing solutions ( chloramine and alco-hol) .Functional healing was found significantly more frequent in canines and premolars.Conclusion:The factor significantly affecting periodontal ligament healing is storage medium.Replacement resorption is the most common type of root resorption.The replacement resorption diagnosis must combine the radio-graphic examination with the clinical examination.It is better to follow up more than 1 year after tooth re-plantation.

AIM: To explore the mechanism of the therapeutic effect of hypobaric hypoxia on allergic asthmas guinea pigs. METHODS：After the model of asthma was established with ovalbumin(OA) challenge in OA sensitized guinea pigs, the animals were randomized into the asthmas group(AG), the alleviative group(ALG) and hypobaric hypoxia treated group(HHTG). The levels of plasma cortisol and endothelin(ET), ET in bronchoalveolar lavage fluid(BALF) were determined by radioimmunoassay. The pulmonary pathological changes were observed with optical microscope. RESULTS:(1) Infiltration of eosinophils (EOS) in alveolar septum was found in AG and ALG, while it was reduced in HHTG. (2) The level of plasma cortisol was significantly higher in AG than in normal control group(NCG) (P＜0.01).But it was significantly lower in ALG than in NCG(P＜0.01), there was no difference in plasma cortisol level between HHTG and NCG. (3) The content of ET in plasma was significantly higher in ALG than that in NCG, AG and HHTG(P＜0.01), and ET level in BALF was significantly higher in AG than that in NCG, ALG and HHTG(P＜0.05),however, no significant difference was found among the latter three groups, respectively. CONCLUSION: After the treatment with hypobaric hypoxia, the ET levels of the plasma and BALF were decreaced and the content of plasma cortisol was increaced, and infiltration of EOS in alveolar septum was decreaced in asthmatic guinea pigs.That may be one of the mechanisms by which hypobavic hypoxia prevents and cures asthma.

Objective To study the effect of ahylysantinfarctase (AF) on the growth of fetus lens epithelial cells (LECs) in vitro. Methods Fetal lens anterior capsule membranes from the eyeballs of dead fetus delivered by induced labor underwent primary cell culture with the modified method. The second or third generations of fetal LECs were used in this experiment. They were exposed to AF of six kinds of concentrations (2?10~ -4 , 4?10~ -4 , 8?10~ -4 , 16?10~ -4 , 32?10~ -4 , 64?10~ -4 u/mL). Their morphologic changes were observed with the inverted microscope and the value of absorption (A) in each well was tested by MTT assay to examine the effect of AF on the growth of the fetal LECs collected. Results In the experimental groups, especially AF groups of higher concentration, LECs exhibited inordinate shape, less kytoplasm, more cellular endoparticles, wider intercellular space and random disposition, and even adherence failure. After the cells grew in AF of different concentrations for 48h, A value in each group decreased with the increase of AF concentration, and differed significantly between all the experimental groups (P

Objective To study the joint effect of small dosages of Aspirin and compound Danshen dropping pills on type 2 diabetes(T2DM) patients'and impaired glucose tolerance(IGT) patients' hypercoagulation blood.Methods The study followed diagnostic standards for T2DM by World Health Organization (WHO) in 1999,and randomly selected two groups of clinical patients:one had 63 patients with IGT and the other contained 68 patients with T2DM.Both groups took compound Danshen dropping pills with the total dosage of 10 pills each time,3 times per day,for continuum of 3 months.After that both groups stopped medication for 7 days,then began taking Aspirin after meal,with the dosage of 100 mg per day,for a continuum of 3 month.After that both groups stopped the medication for 7 days,then both group continued to take same dosage of ASA with addition of compound Danshen dropping pills of total amount of 10 pills per each time,3 times per day,for acontinuum of 3 months.Each medication period (ev ery 3 months) was monitored for platelet count (PET),prothrombin time (PT),kaolin partial thromboplastin time (APPT),The effective rate of anticoagulation for both groups were then calculated.Results The difference of PT、APTT between before and after taking single dosage of ASA for patients with impaired glucose tolerance (IGT) was statistically significant (P＜0.05),while patients with T2DM had no significant difference before and after taking same single dosage of ASA.Similarly, both groups with single dosage of compound Danshen dropping pills had no significant difference between before and after taking the medication.However,the difference of PT、APTT between before and after taking the combination of Aspirin and Danshen dropping pills of both groups were statistically significant (P＜0.05).IGT group with single dosage of ASA had an effective rate of 71.4％ for anti coagulation,while T2DM group with the same dosage reached 23.8％.The IGT group with combinatory dosage of ASA and Danshen dropping pills reached an effective rate of 100％ for anticoagulation,while T2DM group with the same dosage was 86.7％.The difference between those effective rates of anticoagulation was statistically significant in different medicine takeways of two groups (P＜0.05).Conclusion The combinatory use of small dosage of Aspirin with compound Danshen dropping pills had evident effect to improve IGT and hypercoagulation blood of T2DM patients who had good control of their blood glucose.

Biocontrol bacteria 308R(pKSH) with hrpN of Erwinia amyloyora. It can secrete the Harpin protein which can induce plant resistance. After successive growth in antibiotic-free LB medium for 50 generations, only 23.1% of the cell still retained the plasmid pKSH, in comparison with 4.75% of the cosmid pCPP430. Sprayed onto the leaves of tomato, the recombinant strain maintained a population density of over 10~ 5 cfu/cm~ 2 when kept under high humidity in thirteen days, but if without humidity, the strain keep 10~ 4 cfu/cm~ 2 over in five days. On the tomato leaves, the stability of the recombinant strain 308R(pKSH) was higher than the strain 308R(pCPP430). The experiment indicated that the recombinant strain 308R(pKSH) had higher stability compared with the strain 308R(pCPP430), but that was not enough. In this paper reasons for unstability and strategies for improving were discussed for the recombinant strain.

Objective: To explore levels of salivary IgA, lysozyme, lactate dehydrogenase, alkaline phosphatase and total proteins in unstimulated (UWS) and stimulated (SWS) whole saliva of caries-free children. Methods: 94 caries-free children, aged from 42 to 54 months, were recruited from urban kindergartens in Beijing. 2 ml UWS and 2 ml SWS were collected from each child to measure salivary IgA, lysozyme, lactate dehydrogenase, alkaline phosphatase and total proteins. Results:1) There were no differences in salivary proteins between boys and girls in both UWS and SWS, except total proteins. In UWS, the concentration of total proteins in girls was lower than that in boys (P0.05). 2) The concentration of IgA in SWS was significantly lower than that in UWS (P0.05). Conclusion:In 3-to 4-year old children, the protein composition in stimulated whole saliva is different from that in unstimulated whole saliva, and it is also different between genders. Therefore, it is more reliable to measure salivary proteins in both UWS and SWS when studying saliva of children.