2.MicroRNA profiling on oxidative stress induced apoptosis of human lens epithelium
Ling, BAI ; Peng, LI ; Ling, CHEN ; Ling, HE ; Feng, WANG
International Eye Science 2014;(10):1770-1772
AIM: To identify the changes in microRNA ( miRNA ) profile of human lens epithelium ( HLE) induced by H2 O2 and the role of miRNA in oxidative stress induced apoptosis.
METHODS: HLE cell line HLE - B3 was treated by 100μmol/L H2 O2 for 24h and the total RNA were isolated by Trizol reagent. miRNA profile was generated by miRCURYTM LNA microRNA Array. The target genes of differentially expressed miRNAs were predicted by bioinformatics software.
RESULTS:Twenty-eight miRNAs showed significantly differential expression after H2 O2 treatment, 18 miRNAs upregulated and 10 miRNAs downregulated. The differentially expressed miRNAs may involve in apoptosis of lens epithetium and development of cataract through targeting BCL2L2 and MIP.
CONCLUSION: H2 O2 can induce dramatically changes in miRNA profile of HLE, which may play a pivotal role in the pathogenesis and development of cataract.
3.Effect of shear stress on the expression of growth factors in endothelial cells around stent edge
Ancai WANG ; Lifang LI ; Buchun ZHANG ; Ling BAI
Chinese Journal of Tissue Engineering Research 2007;11(44):8992-8996
BACKGROUND:Alterations in secondary hemodynamics around the stent edge can be caused by stent planted. Is there different effect of shear stress on platelet growth factors-A, B (PDGF-A, B) and basic fibroblast growth factor (bFGF) of endothelial cells around stent edge after stent planted?OBJECTIVE: To explore the effect of shear stress on growth factors of endothelial cells around stent edge.DESIGN: Observational comparative study.SETTING: Department of Cardiology, Yijishan Hospital, Wannan Medical College; Laboratory of Biomechanics (National Laboratory), Medical College of Shanghai Jiao Tong University.MATERIALS: The experiment was carried out in the Laboratory of Biomechanics, Medical College of Shanghai Jiaotong University from April to October 2006. The main reagents were detailed as follows: trypsin (Hyclon Company); M-199 medium (Gibco BRL Company); fetal bovine serum (Hangzhou Sijiqing Bioengineering Material Institute); aFGF, Heparin and Hepes (Sigma Company); thymide, Ⅷ factor monoclonal antibody (Rabbit anti-human),biotinylated horse anti-rabbit IgG and propidium lodide (Sigma Company); neonate belly band (Delivery Room of Shanghai the Fifth People's Hospital); trizol (Invitrogene Company); reverse transcription polymerase chain reaction (RT-PCR) kit (Fermentas Company); penicillin/streptomycin (AB/M), objective gene primer (Shanghai Bioengineering Company).METHODS: Traditional parallel-plate streaming cavity was modified into rectangular and gradient shear stress models;while static group was established at the same time. Two shear stress groups were subjected to 11.37 dyne/cm2 and 5.66-14.38 dyne/cm2 shear stress, respectively for 3 hours, 6 hours and 12 hours.MAIN OUTCOME MEASURES: The expressions of PDGF-A, B and bFGF mRNA of human umbilical endothelial cells (HUVECs) were measured by RT-PCR at different time points.RESULTS: Compared with static group, the maximal expression of PDGF-A, B and bFGF mRNA was reached peak at 3 hours in gradient shear stress group and this level of PDGF-A mRNA expression remained till 12 hours. in rectangle shear stress group, the peak value of mRNA expression of these growth factors was all reached at 6 hours, and then gradually decreased.CONCLUSION: Over-expressions of PDGF-A, B and bFGF induced by gradientshear stress after stent planted may play an important role in stent restenosis through intimal hyperplasia.
4.Effects of inhalation of different concentrations of sevoflurane combined anesthesia on somatosensory evoked potentials in adolescent patients undergoing scoliosis surgery
Xue BAI ; Zhihua LI ; Lin ZHU ; Ling ZHU
Chinese Journal of Anesthesiology 2012;32(7):811-813
Objective To investigate the effects of inhalation of different concentrations of sevoflurane combined anesthesia on somatosensory evoked potentials (SEPs) in the adolescent patients undergoing scoliosis surgery.Methods Forty-five adolescent patients of both sexes,aged 12-17 yr,with body mass index of 15-23 kg/m2,undergoing scoliosis surgery under general anesthesia,were randomly divided into 3 groups ( n =15 each):low concentration sevoflurane group ( group S1 ),moderate concentration sevoflurane group ( group S2 ) and high concentration sevoflurane group (group S3 ).Anesthesia was induced with fentanyl 4 μg/kg,midazolam 0.03 mg/kg,propofol 2.0 mg/kg,and rocuronium 0.6 mg/kg.The patients were endotracheal intubated and mechanically ventilated.Anesthesia was maintained with propofol infusion at 8 mg· kg-1 · h-1 and remifentanil infusion at 0.25 mg· kg-1 ·min-1.After beginning of the surgery,1.8%,4.0% and 6.0% sevoflurane were inhaled for 10 min in groups S1-3 respectively,SEPs were measured by stimulating the posterior tibial nerve and significant SEP suppression was detected and recorded.The wave P40 latency,wave P40-N50 amplitude,and time for P40 waveform changes were recorded before sevoflurane inhalation (baseline) and at 10 min of sevoflurane inhalation,and the time for recovery of P40 waveform was recorded after sevoflurane inhalation was stopped.The prolonged percentage of P40 latency and decreased percentage of P40-N50 amplitude were calculated.Results The rates of significant SEP suppression were 80%,100% and 100% in groups S1,S2 and S3 respectively ( P > 0.05).Compared with group S1,the prolonged percentage of P40 latency and decreased percentage of P40-N50 amplitude were significantly increased,the time for P40 waveform changes was significantly shortened,and the time for recovery of P40 waveform was significantly prolonged in groups S2 and group S3 ( P < 0.05).Compared with group S2,the decreased percentage of P40-N50 amplitude was significantly increased,the time for P40 waveform changes was significantly shortened,and the time for recovery of P40 waveform was significantly prolonged in group S3 ( P < 0.05).Conclusion 1.8%,4.0% and 6.0% sevofiurane combined anesthesia can suppress SEPs in the adolescent patients undergoing scoliosis surgery,and they are not suitable for the surgeries requiring SEP monitoring.
5.Research on the training practice of part-time assistant teachers in clinical teaching bases at western medical colleges of China
Dongye YANG ; Ling BAI ; Lizhen ZHANG ; Yingqiong ZHOU ; Li SUN
Chinese Journal of Medical Education Research 2011;10(7):813-815
It is an important measure in improving the practical teaching quality at Chinese westem medical colleges to strengthen the training practice of part-time assistant teachers in clinical teaching bases. To establish a good training model,we should take into consideration its operability and practicability, as well as the positivity of the teachers.The key to making the training succeful is to ascertain the direction and contents, to set up an instructors' team, and to make a specific training plan.
6.A case of congenital leukopenia.
Hong-xiang LI ; Xiao-ling BAI ; Nan ZHENG
Chinese Journal of Pediatrics 2005;43(3):191-191
Humans
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Infant, Newborn
;
Leukopenia
;
complications
;
congenital
;
diagnosis
;
Male
7.Polypoid excrescences of colonic mucosa: report of two cases.
Bai-Zhou LI ; Tian-Rong XU ; Yi-Ling WANG
Chinese Journal of Pathology 2007;36(11):750-750
Colon
;
pathology
;
Colonic Polyps
;
pathology
;
Diagnosis, Differential
;
Female
;
Humans
;
Intestinal Mucosa
;
pathology
;
Male
;
Middle Aged
8.The Expression of PP2Ac in Various Kinds of Lung Carcinomatous tissue and Lung Carcinomatous cells
Caixia LI ; Ling ZHANG ; Peng BAI ; Qinqin WANG
Journal of Kunming Medical University 2006;0(06):-
0.05),that there was negative expression of PP2Ac protein in the cytoplasm of the human lung squamous carcer and small cell lung cancer while positive expression of PP2Ac protein in intercellular substance,and that there were negtive expression of PP2A in human lung adencarcinoma and overexpression of p-AKT protein in human lung adencarcinoma.Western blot technique analysis showed that there was nomal expression of PP2A protein in normal human lung tissue,and lower expression in YTLMC-90 cell than in nomal human lung tissue.While sharp low expression GLC-82 cell.Adding okadaic acid(OA),an inhibitor of PP2A,the expressiong of p-AKT in GLC-82 cells was sharply higher than in normal lung tissue at 6 h,12 h and 24 h,indicating that inverse correlation between PP2A and p-AKT protein expression.Conclusions PP2A is involved in human lung carcinomar,its expression level is related to histological type of human lung carcer.Activition of AKT(p-AKT)is related tightly to human lung carcer.
9.Effects of Sisheng Decoction on the immunity and anti-stress function in mice with spleen deficiency syndrome.
Sufang ZHANG ; Changquan LING ; Bai LI ; Hongyun CHEN ; Zhe CHEN
Journal of Integrative Medicine 2012;10(12):1465-9
To study the possible mechanism of Sisheng Decoction on spleen deficiency syndrome via the observation of general conditions, immunity and anti-stress function in Dahuang (Radix et Rhizoma Rhei Palmati)-induced mice model.
10.Effects of a formula of components from Shengmai Powder on glucocorticoid receptor in rats after thermal injury.
Xiang LU ; Binbin CHENG ; Changquan LING ; Xiaoqiang YUE ; Bai LI
Journal of Integrative Medicine 2009;7(2):121-4
To investigate the effects of a formula of components from Shengmai Powder, a compound traditional Chinese herbal medicine, on glucocorticoid receptor (GR) in rats after thermal injury.