Objective To determine the expressions of transcription factor activator protein-2α (AP-2α), estrogen receptor-β (ER-β) and matrixmetalloproteinase-9 (MMP-9) in lichen planus (LP) lesions and their significance. Methods Tissue samples were resected from the lesions of 30 patients with LP and normal skin of 30 human controls. An immunohistochemical method using streptavidin-peroxidase (SP) was performed to detect the expression of ER-β and MMP-9, reverse transcription-PCR and Western blot to measure the mRNA and protein expressions of AP-2α, ER-β and MMP-9 in these specimens. Results There was a significant decrease in the mRNA expressions of AP-2α (0.488 ± 0.039 vs. 1.008 ± 0.023, P < 0.01 ) and ER-β (0.365 ± 0.032 vs. 0.998 ± 0.036, P < 0.01), together with an increase in the expression of MMP-9 (1.237 ± 0.027 vs. 0.567 ± 0.015, P< 0.01) in the LP lesions compared with the control specimens; similar results were observed for the protein expressions of AP-2α, ER-β and MMP-9. In LP lesions, the expression of AP-2α was positively correlated with that of ER-β (R = 0.89, P < 0.01), but negatively correlated with that of MMP-9 (r = -0.91, P < 0.01). Conclusions The down-regulation of ER-β expression and up-regulation of MMP-9 expression may be ascribed to the abnormal regulation of upstream target genes mediated by the decreased expression of AP-2α, which may be involved in the pathogenesis of LP.

Objeetive To investigate the relationship between 24-hour blood pressure rhythm and carotid scleratheroma in elderly patients with hypertension.Methods 193 elderly patients with hypertension were enrolled in the study and divided into study group (91 cases with carotid atherosclerosis) and control group (102 cases).All the patients were given 24h blood pressure rhythm examination.The characteristics of 24hour blood pressure rhythm was compared between the two groups.Results The 24h average systolic blood pressure(24h-SBP),24h average diastolic blood pressure (24h-DBP),24h average pulse pressure (24h-MBP) between the study group and the control group had no significant differences(P ＞ 0.05).The daytime average systolic blood pressure(d-SBP),diurnal mean diastolic blood pressure (d-DBP),diurnal average pulse pressure (d-MBP),nocturnal mean systolic blood pressure (n-SBP),nocturnal mean diastolic blood pressure(n-DBP),nocturnal mean pulse pressure(n-MBP) of the study group were significantly higher than those of the control group (t =16.92,24.68,6.16,12.87,11.59,6.35,all P ＜0.05).In study group,d-SBP,d-DBP,d-MBP were higher than n-SBP,n-DBP,n-MBP,the differences were statistically significant(F =73.61,P ＜ 0.01).The circadian blood pressure variability of the study group was more than 10％,the 24h ambulatory blood pressure curve graph of the study group was non-dipper curve.The circadian blood pressure variability of the control group was less than 5％,the 24h ambulatory blood pressure curve was dipper type curve.Conclusion The MBPS is strongly associated with carotid scleratheroma,and clinical treatment and study could derive from ABPM data analysis.

The centrosome plays a crucial role in the maintaining of cell conformation,mitosis and chromosome seg-regation.It responds to the DNA damage and keeps the genome stability via cross-talking with the intranuclear DNA damage repair system.The apoptosis of tumor cells can be induced through inhibition of the duplication of centro-some.So the inhibitors of centrosomal proteins will be a potential anti-tumor therapy.

Arthritis, Juvenile ; diagnosis ; therapy ; Humans ; Practice Guidelines as Topic

Cancer-related fatigue survivors have been found to have lower morning serum cortisol levels, flattened diurnal cortisol cy-cles, and decreased cortisol responses to psychological stress. The mechanism may be related to the inflammation caused by disorder of hy-pothalamus-pituitary-adrenal axis, and there are also some evidences to support that cortisol can be used to reduce cancer-related fatigue. This study introduced the research progress of the relationship between cancer-related fatigue and hypothalamus-pituitary-adrenal axis.

The study process of TCM syndrome spectra of hemopathy is the process to recognize the syndrome characteristics of the onset, development of hemopathy, it bases on norm of TCM symptoms and syndrome. In this course, it could not only find common feature of syndrome,but also analyze distribution regularity of TCM syndrome. Thus to establish sequence of hemopathic syndrome, and to provide the basis for clinic determination of treatment based on syndrome differentiation, and provide the foundation for finding correlation of TCM syndrome and target tests.

The fresh cadavers were injected with A. B. S into the popliteal arteries. We have studied the micro-vascular structure of this skin area under scanning electron microscope. There are 5 layers of the vascular network in this skin area. They were formed by the small branches coming from either the direct or indirect cutaneous arteries. The 5 layers are as follows: The capillary network of the papillary layer. The vascular network of the subpapillary layer. The deep dermal network. The network in the subcutaneous tissue. The network in the deep fascia tissue. It is clear that each layer of the vascular network has it's -own special character.

The vascular morphological character of the microcirculation in the nail bed of the toe have been studied with the scanning electron microscope. Three types of vascular network were identified: 1) The blood vessels at the proximal two fifth of the nail bed were parallelly arranged. 2) At the middle two fifths of the nail bed, the blood vessels interwoved with each other and formed the polygonal vascular networks. 3) At the distal one fifth of the nail bed there were only a few vascular loops running in a slanting position. These loops were connected with the vascular network from the papillary layer of the skin of the toe.

To study the changes of ? adrenergic receptor subtypes (? 1 AR,? 2 AR) and angiotensin Ⅱreceptor subtypes (AT 1 R,AT 2 R)mRNA levels in spontaneously hypertensive rat (SHR) hearts at 14 weeks of age, and WKY rat hearts at ages 14 and 48 weeks, the total cellular RNA was extracted from the left ventricular tissue, and reverse polymerase chain reaction analysis was performed for measurement of the four receptors. The expression levels of ? 1 AR mRNA and ? 2 AR mRNA were down regulated in WKY hearts at age 48 weeks and in 14 week SHR. AT 1 R mRNA levels were decreased in 48 week WKY and 14 SHR, and AT 2 R mRNA levels were significantly increased in SHR. The expression levels of ? 1 AR and AT 1 R mRNA were declined following the increase of ages. The levels of ? 2 AR mRNA were down regulated in hypertension and myocardial hypertrophy conditions first because ? 2 AR was more sensitive to myocardial hypertrophy than ? 1 AR. Long term and hyper AngⅡ levels stimulated AT 1 R mRNA down regulation in SHR, and it is the myocardial hypertrophic compensatory process that increases the AT 2 R mRNA levels.