In recent years,the efficacy of cervical cancer treatment has been increasing gradually.It has got a basic consensus of the standard treatment mode for most cervical cancer,but further effort should be done to improve the treatment response of locally advanced cervical cancer and recurrent or metastatic cervical cancer.The efect of redical surgery and radiotherpy is the same to early cervical cancer.Concurrent chemoradiotherapy is the standard treatment for patients with locally advanced cervical cancer.Chemotherapy combined with antiangiogenic targeted therapy can improve the survival of patients with recurrent or metastatic cervical cancer,while whether needing new adjuvant therapy or not,it asks for further investigation.

In order to select a computed tomography(CT)scanner system with reasonable cost performance (performance-price ratio) among various brands and types of CT products in accordance with the clinical features of the pediatric patients, Tianjin Children's Hospital made a feasibility study on purchasing the equipment in all its aspects, including the evaluation of its type and performance, social and economic benefits, and eventually purchased a 16-slice helical CT scanner which fully fulfills the practical needs of the hospital. This way of evaluation can be widely used in the feasibility study for purchasing different large-scale medical equipment to avoid unnecessary waste of funds.

Objective To assess atrophy of the multifidus in elderly patients with low back pain using magnetic resonance imaging.Methods Thirty patients with low back pain were recruited,and 15 healthy subjects formed a normal group.The cross sectional area (CSA) and signal strength of the multifidus muscles at the L4-5 and L5-S1 levels were measured along with the signal strength of the hip fat for all of the subjects in both groups using a superconducting magnetic scanner.Results There was no statistically significant CSA difference between the groups at either level.The contrast between multifidus muscle and adipose tissue was significantly different between the groups at both levels.Conclusion In evaluating atrophy of the multifidus muscle in elderly patients with low back pain,the contrast between multifidus muscle and adipose tissue at either level L4-5 or L5-S1 can be used for assessment.It is more reliable,specific and sensitive than the CSA of the muscle.

Adult ; Blood Viscosity ; Coronary Disease ; blood ; Diabetes Mellitus ; blood ; Diagnosis, Differential ; Female ; Humans ; Male ; Medicine, Chinese Traditional

Objective To investigate the clinical characteristics of Kennedy disease with secondary mitochondrial dysfunction and glutaric aciduria typeⅡ.Methods The clinical data of 1 case Kennedy disease with secondary mitochondrial dysfunction and glutaric aciduria typeⅡwas retrospectively analyzed.Results The patient presented muscle weakness in proximal limbs, fasciculation, bulbar palsy, amyotrophy, postural tremor, sensory disturbance, gynaecomastia, impotency.The level of serum creatine kinase and urinary glutaric acid were elevated. Electromyogram and nerve and muscle biopsy supported mixed damage in peripheral nerves, as well as neurogenic and secondary myogenic pathologic changes in muscles.Gene sequencing indicated trinucleotide CAG repeated amplification for 47 times in chromosome X.Diabetes was diagnosed at 7 years after onset.Conclusions The pathogenesis of Kennedy disease is very complex, which may involve myogenic factor.The treatment to the secondary mitochondrial and lipid metabolic disturbance in muscle can improve the muscle weakness to a certain extent.

ObjectiveTo investigate the change of the platelet function in children with primary immune thrombocy-topenia (ITP) before and after treatment.MethodsThe platelet indices, immature platelet fraction (IPF%), immature platelet counts (IPC), granule membrane glycoproteins of platelet (CD62p, PAC-1, CD42b) were tested by automatic blood cell analyzer and lfow cytometry (FCM) in 18 children with ITP before treatment and after complete response and in 17 children undergoing elective surgery (control gourp).ResultsCompared with control group, MPV, PDW, P-LCR and IPF% were signiifcantly higher and PLT, PCT, IPC were signiifcantly lower in children with ITP. The expressions of three platelet membrane glycoproteins (PAC-1, CD62p, CD42b) were signiifcantly lower in children with ITP. After treatment, children with ITP achieved completely response. Compared with before treatment, MPV, PDW, P-LCR and IPF% were signiifcantly lower, and PLT, PCT, IPC, and the expressions of three platelet membrane glycoproteins (PAC-1, CD62p and CD42b) were significantly higher. ConclusionsThe platelet function is depressed and in status of low activity in children with ITP. The relative platelet indices provide new references for the diagnosis and the judgment of therapeutic effect in ITP patients.

Objective: To investigate the antitumor effect of SGI-1776 on human ovarian cancer HO-8910 cells and its molecular mechanism. Methods: HO-8910 cells were cultured in vitro, and the proliferation inhibitory effects of SGI-1776 were determined by MTT assay and colony formation assay. The effect of SGI-1776 on the distribution of cell cycle phase was observed by flow cytometry with propidium iodide (PI) staining. hTe inhibition rate of migration and invasion were valued by transwell cell assay. Multiple molecular techniques, such as ELISA, Western blot, siRNA and cDNA transfection were used to explore the molecular mechanism. Results: SGI-1776 presented dramatic anti-tumor activity against HO-8910 cells in vitro, inhibited the cells proliferation and colony formation, and attenuated the migration and invasion in a dose-dependent manner, accompanied by cell cycle arrest in G1 phase. SGI-1776 caused the proliferation inhibition with concomitant decrease in Pim-1 kinase activity, down-regulated the expression of Pim-1 protein and and its downstream genes, such as CDK6, pCDK6, CDK4, pCDK4, CDK2 and pCDK2, and increased the expression of P21 and P27. Down-regulation expression of Pim-1 by siRNA followed SGI-1776 treatment resulted in enhanced cell proliferation inhibition rate and attenuated migration/invasion. Up-regulation of Pim-1 by cDNA transfection attenuated SGI-1776-induced cell proliferation inhibition and its migration/invasion. Conclusion: Pim-1 mediates the biological effect of SGI-1776 in human ovarian cancer HO-8910 cells, suggesting Pim-1 might be a novel target for human ovarian cancer.

After Achilles tendon (AT) rupture operation,traditional gypsum immobilization must lead to AT in a condition of a tension-free or even "disuse"-negative balance healing system,which may inhibit the key opportunity of AT healing process to induce "active" mechanical signals stimulation,and AT "passively or intermittently" accepts the mechanical stimulation even applying the ultrasound,vibration and other mechanical stimulation to AT in the course of treatment. If using the method of active movement after operation,it can accelerate the AT healing,maintain function and promote re-construction of the AT,thereby help to restore the biomechanical properties of AT. Using proteomics techniques can reveal the mechanical signal transduction mechanisms of kinesiatrics promoting the AT healing in the high-throughput molecular level.

Shenghua decoction made by FU Qing-zhu is a famous formula aimed at syndrome of coagulation cold due to blood deficiency,static blood obstruction.The mechanism of disease is definite when this formula is applied.But the extensive use of Shenghua decoction,especially some potent preparation of it following childbirth without according to TCM principle based on differentiation of syndromes is a manifestation of highlighting formula and underestimating theory,which is useless and harmful.It should be emphasized.So the Shenghua decoction should be used under the direction of basic theory of TCM,through precise syndrome differentiation,according to the principle of "looking to and not being besieged by postpartum ".We should not follow blindly the method of treatment of "warming and resolving stasis" on the postpartum disease.It is important that Shenghua decoction should be used on the basis of syndrome differentiation.

Object To study the suitable conditions on tissue culture of stem apex and shoot rapid propagation of ginger. Methods The ginger stem apex ((?)