Objective To design and establish a set of diffusion optical tomography(DOT) system.Methods Near-infrared(NIR) laser was employed as the light source and only one photomultiplier tube(PMT) as the detector.An optical multiplexer was used to alter the detector channels.The 32 channels of the system,which are consisted of 16 launch channels and 16 detector channels,worked under the continuous-wave(CW) model and were used to acquire 256 boundary data.Results Experiments were performed based on the proposed imaging system.The intralipid was used as the tissue-like medium and the India ink as the absorber.Two sets of data on the boundary were sampled,respectively,before and after the absorber was embedded inside the tissue-like medium.The two sets of data were normalized and then used to reconstruct the absorption coefficient distribution.The recovered image reflected the real location and size of the absorber.Conclusion The proposed imaging platform can image the tissue optical parameters effectively.However the resolution of the reconstructed image was not high because the inverse problem was gravely underdetermined and the noise was not considered in the reconstruction algorithm.The recovered result in the next generation system could be improved by making more use of prior information and enhancing the performance of the system.

OBJECTIVE:To test and verify the function of Composite Radix Sophora Flavescentis injection in immunologic enhancement.METHODS:A total of 121 patients with lung cancer were randomly assigned to 2 groups:the treatment group(n=61)received common chemotherapy for 6wk plan in combination with Composite Radix Sophora Flavescentis injection(20mL,iv gtt)qd for the first 10 to 14 days,while the control group(n=60)received common chemotherapy alone for 6wk.The indexes of immunity in both groups were detected before and after treatment.RESULTS:The index of immunity in the treatment group increased significantly as compared with before treatment(P

Aim To investigate the effects of propafenone on myocardium inotropism and explore it’s possible mechanism in isolated papillary muscle of guinea pig. Methods Developed tension (DT), maximum rate of contraction (+dT/dt_ max) and maxi-mum rate of relaxation (-dT/dt_ max) were measured during propafenone perfusion before and after administration of L-type calcium channel blocker, nicardipine and selective Na+/Ca 2+ exchanger inhibitor, KB-R7943. Results ①At concentration of 0.1,1,10,30 ?mol?L -1,propafenone attenuated DT from control (0.18?0.05) g to (0.14?0.03), (0.12?0.03), (0.08?0.02), (0.05?0.02) g respectively (P

Objective To explore relationships of expression of hypoxia?inducible factor?1α(HIF?1α), vascular endothelial growth factor(VEGF)and protein kinase B(P?Akt)with angiopoiesis and cell apoptosis. Methods Biopsy specimens were collected from skin lesions of 32 patients with lichen planus and normal skin of 20 patients with lipomyoma, and subjected to paraffin embedding. Immunohistochemical staining was performed to measure expression of HIF?1α, VEGF and P?Akt, and TUNEL technique was used to detect apoptosis of keratinocytes in these paraffin?embedded tissue sections. Microvessel density (MVD)was assessed by counting CD34?labeled vascular endothelial cells. Results HIF?1α, VEGF and P?Akt were moderately or strongly expressed in lichen planus lesions, but absent or weakly expressed in normal skin of controls, and the expression of HIF?1α, VEGF and P?Akt was significantly higher in the lichen planus group than in the control group (all P < 0.01). HIF?1α was mainly expressed in nuclei of keratinocytes, while VEGF and P?Akt were expressed in the cytoplasm of keratinocytes. In addition, the lichen planus group showed significantly increased MVD(21.27 ± 6.54 vs. 10.26 ± 1.10 microvessels/high?power(200 ×)field, t = 5.607, P < 0.01)and apoptosis rate of keratinocytes(72.81% ± 9.234% vs. 28.16% ± 3.464%, t = 8.431, P < 0.01) compared with the control group. Pearson correlation analysis showed that there were positive correlations between HIF?1αand VEGF expression, between VEGF and P?Akt expression, and between P?Akt and HIF?1αexpression in the lichen planus group(r=0.625, 0.453, 0.455, respectively, all P<0.01), and expression of HIF?1α, VEGF and P?Akt was all positively correlated with MVD(r=0.721, 0.646, 0.671, respectively, all P<0.01). Conclusion HIF?1αand its downstream target genes VEGF and P?Akt may play a certain role in the occurrence of lichen planus.

Objective To evaluate the effect of a new one-piece PHEMA keratoprosthesis(Kpros) implanted in alkali burned rabbit corneas. Methods The experiment included two parts. In part one, the porous discs of PHEMA were inserted into the lamellar pockets of alkali-burned corneas and the results were observed clinically and histologically at various periods. In part two, seven one-piece Kpros were implanted into alkali burned rabbit corneas. The Kpros consisted of a central transparent optical area and a porous semi-transparent peripheral skirt. The surgical procedure was similar to that of penetrating keratoplasty. Clinical observations were carried out till 28 weeks. Results Both the porous discs and one-piece Kpros had been retained during the experiment. Severe complications as cataract, retro corneal membrane, retinal detachment and extrusion of material were not found until 28 weeks. There was no calcification in the samples, and inflammation was mild. Two weeks after implantation, fibroblasts and deposition of collagen were found in the pores of PHEMA discs. The fibroblasts in the pores look healthy and functionally active. The preliminary results were satisfactory. Conclusion This new one-piece Kpros had shown good biocompatibility. The porous peripheral skirt fuses well with the bed of burned cornea and proved to be stable. PHEMA materials is suitable for one-piece Kpros.

Cell apoptosis is an important biological process. It plays an essential role on cell growth and response for outside stimulations. This review focused on the recent advancement of apoptotic signal transduction, enzymological mechanisms, the function of mitochondria in cell apoptosis as well as the regulation of genes.

Objective:Our purpose was to establish an ideal chronic pressure-afterload heart failure rat model which has the transition from cardiac hypertrophy to heart failure. Methods: Chronic pressure-afterload heart failure rat model was induced by gradually constricting the ascending aorta of young rats. Young rats were randomly divided into 2 groups: the constricted and sham-operated groups. Clinical manifestation, tail-cuff blood pressure, organ weight, and hemodynamic data were observed at various time after operation. Results: The overall survival rate was 87%. Tail-cuff pressure began to increase in 4 weeks after operation. Left ventricular hypertrophy appeared in 12 weeks and heart failure in 5 months. Conclusion:It's a practical and reproducible model of cardiac hypertrophy that progresses to chronic heart failure.

Objective To investigate the efficacy of sorafenib alone or combination with transarterial chemoembolization(TACE)and percutaneous local cryotherapy(PLCT)for advanced hepatocellular carcinoma patients without operation opportunity. Methods Sixty-four advanced hepatocellular carcinoma patients were selected as our subjects,who were underwent treatment of sorafenib alone or combination with TACE and PLCT. Thirty-two cases with sorafenib therapy were served as sorafenib group and another 32 cases with sorafenib in combination with transarterial chemoembolization and PLCT were served as combination group. All patients were followed up for 6 - 32 months. The treatment efficacy and tumor development were recorded. Results All surgeries of the patients were succeed and no death or serious operation complications occurred. Of 64 patients, 11 were achieved a complete remission( CR),31 cases with partial remission( PR),14 cases with stable development(SD),and 8 cases with progressive disease(PD). In the sorafenib group,3 cases were with CR,11 patients with PR,12 with SD,and 6 patients with PD. In the combination group,8 patients were with CR,20 patients with PR,2 patients with SD and 2 patients with PD,and the difference was significant between the two groups(χ2 = 14. 028,P = 0. 003). The median periods to tumor progression were 20 and 53 weeks in the sorafenib group and the combination group,and the difference was significant( χ2 = 14. 773,P = 0. 000). Conclusion For hepatocellular carcinoma patients without operation opportunity,sorafenib combined with TACE and PLCT can increase the tumor remission rate and prolong the periods to tumor progression in patients with hepatocellular carcinoma.

Objective To investigate the clinical effect of hysteroscope combined with B-ultrasound in the treatment of uterin cavity adhesion. Methods Hegar dilatior or electric knife of hysteroscope was used to cut adhesion, which was monitored by B-ultrasound. Antibotics, artificial hormonal cycle treatment and IUD in uterin cavity were used after operation. Results 55 cases of uterin cavity adhesion were separated completely and had no complication. Amenorrhvea or rare menstrual flow occurred in 48 cases,and menstrual flow recovered in 43 cases (89 6%) . 24 cases of dysmenorrhvea relieved. 21 of 36 patients who hoped for pregnancy became pregnant(58.3%).The pregnant rate of patients with light uterin cavity adhesion was highest, followed by moderate uterin cavity adhesion, and there was no pregnancy in the cases of serious uterin cavity adhesion(? 2=6 826, P =0 033). Conclusions Hysteroscope combined with B-ultrasound to treat uterin cavity adhesion is a preferable method. Pregnancy after operation was closely related to degree of uterin cavity adhesion.

Objective To evaluate the therapeutic effect of percutaneous vertebroplasty good cement dispersion within the vertebral body fracture line.Methods The clinical data of 105 osteoporotic compression fracture patients with single vertebral fractures treated by vertebroplasty were retrospectively analyzed.Including 51 males,54 females,aged 61 to 82 years,mean 75.18 years,duration of 1d to 2 months,compressed segments distributed in the T9-L3.Preoperative patients received X-ray film,routine CT scan and reconstruction and MRI examination to determine the responsibility of patients with vertebral fracture line position,intraoperative puncture position to the fracture line in the C arm guidance,and bone cement was injected,puncture used unilateral or bilateral,injection of bone cement in an amount of 3-5mL.The visual analogue pain score(VAS)was recorded before and after surgery for 48h and at the last follow-up.Results All patients were successfully completed without serious complications,intraoperative real-time C-arm fluoroscopy and postoperative review X-ray showed that bone cement in the fracture line was well dispersed.Operation time was 25-50min,average 36min.The amount of injection of bone cement within the vertebral body was 3-5mL,average 3.6mL.2 cases of bone cement leakage vertebrae forward,leakage to posterior vertebral body(vertebral canal)in 1 cases,cement leakage rate was 2.8%(3/105),no clinical symptoms.The follow-up period was 6-12 months and the mean follow-up was 7 months.The VAS score between postoperative 48 h and before operation had statistically significant difference[(2.3±0.4)points vs.(7.7±0.7)points,t=-38.72,P<0.05].The VAS score of the last follow-up was(2.2±0.5)points,which was significantly different compared with that before operation(t=-39.21,P<0.05).Conclusion PVP can effectively alleviate the pain caused by osteoporotic compression fractures and improve dysfunction,bone cement manipulation operation preferably dispersed in the fracture line can better improve efficacy.