Background and Objectives: The primary objective of this study is to investigate the necessity of questioning virtual reality systems in the motion sickness susceptibility questionnaire (MSSQ)-short form. The secondary objective of this study is to determine the validity and reliability of the Turkish version of the MSSQ-short form, with proven validity and reliability. Subjects and Methods: In the questionnaire form, for which expert opinion was obtained to maintain linguistic equivalence, the virtual reality items were added to the questionnaire. The questionnaire was then administered to 297 individuals. The results were statistically analyzed with and without these virtual reality items for validity and reliability. Results: After the addition of the virtual reality items, the reliability of the questionnaire was found to be quite high (Cronbach’s alpha r=0.912). The norm values between the original MSSQ-short form (12.9±9.9) and the Turkish MSSQ-short form (13.8±12.9) were found to be consistent. Conclusions Motion sickness symptoms can occur not only during movement, but also with indirect stimulus. Our findings suggest that adding virtual reality items to the original form is important in long term practical applications. Our results show that the Turkish version of the original questionnaire is quite reliable. Submission of the MSSQ-short form in Turkish will be useful for documentation and will also encourage further research in this area.