Although wound healing is a natural biologic process by which the homeostasis is preserved, the resultant hypertrophic scar or scar contracture may produce undesirable morphologic and functional sequelae. To overcome these problems, a number of attempts to produce skin substitute have been made in recent years, but none have proved to be entirely satisfactory. We compared the wound healing of full-thickness dorsal skin defects between ADM(acelluar dermal matrix)-applied wound and ADM non-applied wound group in 20 adult female Sprague-Dawley rats. The wound area was serially evaluated at 1,2,3 weeks and the difference in the mean area ratios between the two groups was then statistically analyzed using Wilcoxon rank sums test. We also performed histological evaluation of the wounds at 1,2,3 weeks. Results were as follows: 1. Experimentally produced ADM was easy to handle. Histologic findings showed no epithelium, no appendages, no cells dermal and subcutaneous layer and pure bundles of collagen. 2. The wound area ratio of ADM applied wound group was statistically larger than that of ADM non-applied(control) wound group. That is, ADM was able to inhibit the wound contraction compared with the ADM non-applied wound group. 3. In the ADM-applied group, we observed low grade exudates, no infection, fine take-up of ADM on full-thickness skin defect, gross and histologic findings of neovascularization, and progressive epithelization on ADM. These findings of biologic attachment implies the function of the ADM as a dermal substitute. These can be basis of the study on the development of artificial skin.
Acellular Dermis* ; Adult ; Animals ; Cicatrix ; Cicatrix, Hypertrophic ; Collagen ; Contracture ; Epithelium ; Exudates and Transudates ; Female ; Homeostasis ; Humans ; Rats* ; Rats, Sprague-Dawley ; Skin* ; Skin, Artificial ; Wound Healing ; Wounds and Injuries*

No abstract available.
Fingers* ; Replantation* ; Transplants*

No abstract available.
Fingers* ; Replantation* ; Transplants*

The purpose of this study is to confirm the effectiveness of stay-suture which is commonly used in the procedure of end to end anastomosis, to find out general biomechanical properties of peripheral nerve with gradual stretching and to evaluate the histological difference after breakage of the nerve. The sciatic nerves of adult rabbits were excised and they were grouped by I and II. Group I was composed of 14 normal sciatic nerves and group II, 14 sciatic nerves sutured with 5-0 nylon after transection. They were stretched on the "Instron" universal testing machine at a constant rate of 200mm/min until breakage took place and the results were compared. The mean maximal tensile strength was 13.6N in group I and 2.1N in group II, representing about one seventh value. The mean ultimate tensile strength was 4.2MPa in group I and 0.9MPa in group II, representing about one fifth value. In load-elongation curve of group I, viscoelastic property was noted as all other biological tissues have. The mean modulus of elasticity was 19 in normal sciatic nerves, which is somewhat lower value than other tissues, indicating superior elastic property of peripheral nerves. Transverse histologic examination showed that epineurium lost its continuity earlier than perineurium, implying perineurium is a more resistant structure than epineurium. However, to confirm this, it is thought to be necessary to examine the nerves serially during distraction at each different status. In conclusion, stay-suture provided about one fifth of ultimate tensile stress, implying its usefullness of resisting excessive tension in end to end anastomosis of peripheral nerves. Normal sciatic nerves showed viscoelastic property with mean modulus of elasticity of 19.
Adult ; Elastic Modulus ; Humans ; Nylons ; Peripheral Nerves ; Rabbits ; Sciatic Nerve ; Tensile Strength

Transarterial chemoembolization is often the first-line treatment for multiple hepatocellular carcinomas. However, hepatic arterial infusion chemotherapy is a treatment option for hepatocellular carcinoma refractory to multiple sessions of transarterial chemoembolization. Hepatic arterial infusion chemotherapy requires implantation of an appropriate port into the hepatic artery. However, it may be impossible to implant a port due to hepatic artery variation. We report a case of hepatocellular carcinoma refractory to transarterial chemoembolization and hepatic artery variation treated successfully with hepatic arterial infusion chemotherapy and radiofrequency ablation with complete response after implantation of ports in both liver lobes.

Ganglion is the most common soft tissue tumor which occurs mainly in hand, but it can also occur in any part of the extremity. It is not always necessary to excise the tumor because it seldom cause symptoms severe enough to take operation. We reviewed the patients with ganglion treated surgically. Exculding the cases which were operated under local anesthesia and Bakers cyst, 42 patients were treated surgically from 1983 to 1992. Females were 33, and males 9. Average age at the time of operation, was 40 years (16 to 67). All the cases were analysed retrospectively in terms of surgical indication, anatomical location, size, duration of symptoms, relationship between recurrence and size, and complication. The average duration of follow-up was 1 year 7 months, ranging from 1 year to 4 years. The causes of operation were, cosmetic problem in 6996 (29 cases); pain in 19% (8 cases); and both comesis and pain in 12% (5 cases). The locations were, wrist in 4696 (19 cases); knee 29% (12 cases); foot 12% (5 cases); hand 7% (3 cases); forearm 2% (1 case); ankle 2% (1 case); and elbow 296 (1 case). The size of mass was less than 2.5 cm in 30 cases (71%), and more than 2.5 cm in 12 (29%). (Total average of size was 2.4 cm)The average duration of symptoms were 28 months (1 month 15 years). The recurrence was found in 7 cases: 3 of them (10%) occurred among 30 cases the diameter of which was less than 2.5 cm, and other four (33%) occurred among 12 cases, larger than 2.5 cm. There were no other complications.
Anesthesia, Local ; Ankle ; Elbow ; Extremities ; Female ; Follow-Up Studies ; Foot ; Forearm ; Ganglion Cysts ; Hand ; Humans ; Knee ; Male ; Recurrence ; Retrospective Studies ; Wrist

In 1912, Crouzon described a syndrome compromising the triad of cranial deformity, facial deformity, and exorbitism. Crouzon's syndrome occurs in 1 in 25,000 live births and follows an autosomal dominant mode of transmission. However 30 to 60% of cases are sporadic and represent fresh mutations. The 27-year-old female patient we report here has family history of two cases of Crouzon's syndrome. The patient had mild nasal obstruction and rhinorrhea, which didn't make the surgery absolutely contraindicated. The Monobloc advancement-Le Fort III osteotomy for midfacial advancement and the lamellar split osteotomy of supraobital bandeau for orbitofrontal advancement- were performed. After 8~12 weeks of patient follow up, CSF rhinorrhea was observed and infection was suspected. The primary focus of infection was supposed to be preexisting sinusitis of the patient. The infection didn't spread intracranially, which was contributed by intact inner table of cranium owing to the lamellar split osteotomy. In conclusion, 1) The importance of irradication of preexisting nasal / perinasal infection such as sinusitis cannot be emphasized too much, 2) How to obliterate the dead space between the inner and outer table, and 3) In terms of infection, at least, lamellar split osteotomy can be regarded superior to classical osteotomy, since inner table serves as a barrier of ascending infection.
Adult ; Congenital Abnormalities ; Female ; Follow-Up Studies ; Humans ; Live Birth ; Nasal Obstruction ; Osteomyelitis* ; Osteotomy ; Sinusitis ; Skull

The reconstruction of the cheek area is a complex problem because the cheek is the most broad area of the face and is close to the aesthetically important region such as eyelids, nose, and mouth. The ultimate purpose of the cheek reconstruction is the restoration of the original shape and function, and establishment of the harmony with the adjacent structure in terms of color, nature and thickness. Various procedures such as skin graft, local flap, free flap, and local flap using skin expansion have been performed for cheek reconstruction. However, no golden principal or indication has been clarified to be imperative. Analyzing our clinical experiences of the cheek reconstruction, we intend to present a general guide of the cheek reconstruction and to assess the advantages and/or disadvantages of the operative methods. Between march of 1989 and August of 1999, we have experienced 154 cases of the cheek reconstruction. There were 52 males and 73 females, and their ages ranged from 3 to 89 years (mean 28.1 years). Preoperative diagnosis were post-burn deformity(n=74), Romberg disease (n=9), post-traumatic deformity(n=7), hemangioma(n=7), nevus(n=7), neurofibroma(n=5), hemifacial microsomia (n=3), lymphangioma(n=3), cancer(n=6), post-hemimandibulectomy(n=1), radical maxillectomy(n=1). The procedures were one stage cervicofacial flap(49 cases), cervicofacial flap following skin expansion(42 cases), local cheek flap (14 cases), deltocervicopectoral flap(2 cases), skin graft(29 cases), free flap(18 cases). Follow up period ranged from 3 to 72 months(mean 11.8 months). Advantages and disadvantages of each procedures were explained. The satisfaction rate was higher in one stage cervicofacial flap and free flap when assessed by both patients and surgeon. There were 3 cases of the ectropion of lower eylids, 4 cases of partial flap necrosis, and 1 case of exposure of tissue expander. The local flap is always the preferred method for the cheek reconstruction and the free flap is the treatment of choice for the cheek contour reconstruction. Selection of the most suitable flap is mandatory depending on the location and size of the defects.
Cheek* ; Diagnosis ; Ectropion ; Eyelids ; Facial Hemiatrophy ; Female ; Follow-Up Studies ; Free Tissue Flaps ; Goldenhar Syndrome ; Humans ; Male ; Mouth ; Necrosis ; Nose ; Skin ; Tissue Expansion Devices ; Transplants

The importance of Toxoplasma gondii in human disease stimulated a number of electron microscope studies on the structure of this protozoan parasite. Gustafson et al. first studied the fine structure by means of thin sections in 1954. Many other papers havs subsequantly appeared. It is well known that Toxoplasma gondii has two stages in its life cycle-the proliferative forms and the cyst. The purpose of the electron microscopical work reported here was to study the fine structure of Toxoplasma gondii with recent techniques clarifying the correlation between the proliferative forms and cyst. RH strain and KM strain as proliferative forms on the one hand and Beverley strain as a cyst form of Toxoplasma gondii on the other hand were used throughout this study. The conoid, toxoneme, nucleus, nucleolus, osmiophilic granules, mitochondria and vacuoles were found in RH strain as wsll as in KM strain and Beverley strain. The endoplasmic reticulum was found in the cytoplasm of RH strain and KM strain. It was better developed in KM strain than in RH strain. The outside contour of the organism of Beverley strain was somewhat irregular and toxoneme of this organism was better developed than in the other two strains. Vacuoles were found in RH strain, KM strain and Beverley strain. Furthermore, tube-like bodies were observed in the vacuoles of the organism of RH strain. In KM strain, two organisms of the same size were demonstrated in the leucocytes. It was presumed that they were products of longitudinal division.
parasitology-protozoa- Toxoplasma gondii ; electron microscopy

The treatment of scar contracture has a relatively long history in the department of plastic surgery. Flap surgery, including Z-plasty and skin graft has been a common surgical modality for the treatment of scar contracture. However, the 'deficiency' of the tissue is basically responsible for the limitations of this treatment. The concept of tissue expansion has contributed to the solution of tissue deficiency and thus tissue expansion is currently widely used for various purposes in plastic and reconstructive surgery. Nevertheless, tissue expansion has not yet clearly solved the problems in cases of seven deficiency of surrounding normal tissues, or in cases of multiple and diffuse scar contracture. We have tried to 'expand the scar tissue' to overcome these limitations. The authors 'several decades of experience in tissue expansion has taught us to find the usefulness of incidentally expanded scar tissues. Thirty-one patients with scar contracture urderwent expansion of surrounding normal and/or scar tissues, including 5 cases of pure scar tissue expansion. The postoperative results were satisfactory with a minimal complication rate. In conclusion, scar tissue expansion was usefully applicable for the scar contracture with no surrounding normal tissues. Scar tissue could be expanded safely and sufficiently, designed to various flaps, and the flap survival was reasonable. The functional and aesthetic quality of the scar tissue seems to improve following tissue expansion. In addition, meticulous planning is essential from the initial operation, including the area of expander insertion, flap design considering the aesthetic unit and functional aspect.
Cicatrix* ; Contracture* ; Humans ; Skin ; Surgery, Plastic ; Tissue Expansion* ; Transplants