1.Analysis of Bone Marrow Micrometastasis using RT-PCR in Patients with Small Cell Lung Carcinoma.
Tae You KIM ; Jong Kook PARK ; Baek Ryeol RYOO ; Yung Hyuck IM ; Yoon Koo KANG
Tuberculosis and Respiratory Diseases 1999;47(6):797-806
BACKGROUND: About 20% of small cell lung cancer (SCLC) patients have bone marrow (BM) metastasis at the time of diagnosis and the remaining patients are also considered with micrometastasis. In an att empt to detect BM micrometastasis, we used cytokeratin (CK)-20 as a molecular marker, which is specific for epithelial cells. METHOD: A sensitive RT-PCR assay was used to compare CK-20 expression both in SCLC cell line H209 and normal leukocyte and to evaluate BM aspirates of 28 SCLC patients. RESULT: H209 cell line showed CK-20 expression but normal leukocyte did not, suggesting CK-20 expression is lung tissue-specific. Of 28 patients (11 limited disease, 17 extensive disease), only 2 (1/11, 1/17) samples tested revealed positive signal for CK-20. Two patients with CK-20 expression had BM metastasis or multiple bone involvement during follow-up. CONCLUSION: Although circulating tumor cells were detected in BM of small portion of patients with bone metastasis, CK-20 doesn't seem to be a reliable marker for the detection of micrometastasis in SCLC. This study emphasizes that identification of more specific marker for micromatastsis is mandatory prior to clinical application.
Bone Marrow*
;
Cell Line
;
Diagnosis
;
Epithelial Cells
;
Follow-Up Studies
;
Humans
;
Keratins
;
Leukocytes
;
Lung
;
Neoplasm Metastasis
;
Neoplasm Micrometastasis*
;
Neoplastic Cells, Circulating
;
Small Cell Lung Carcinoma*
2.Disability Duration in Musculoskeletal Injured Patients due to Automobile Accidents.
Hyun Sul LIM ; Jai Dong MOON ; Choong Ryeol LEE ; Jong Tae LEE ; Yong Hwan LEE ; Baek Geun JEONG ; Young Seoub HONG ; Joon Youn KIM ; Sang Beom KIM ; Kyung Hyun RYOO
Journal of the Korean Academy of Rehabilitation Medicine 2006;30(6):646-654
OBJECTIVE: The aims of this study were to refer descriptive statistics of the disability duration in musculoskeletal injured patients in the automobile accidents. METHOD: The authors chose 469,319 injured patients in traffic accidents as subjects that met inclusion criteria with 8 representative Abbreviated Injury Scale (AIS) comparable with 4th edition of Korean standard classification of diseases. Mean, standard deviation, mode, median, maximum and minimum of 8 representative AIS code were calculated. RESULTS: Mean disability duration for minor cervical sprain was 18.8 days, that of moderate cervical disc herniation was 56.2 days, that of serious cervical disc herniation was 141.5 days, that of minor lumbar sprain was 21.2 days, that of moderate lumbar disc herniation was 53.5 days, that of serious lumbar disc herniation was 128.1 days, that of sprain of 3 major joint in lower limb without desmorrhexis was 24.0 days, and that of dislocation or desmorrhexis of 3 major joint in lower limb was 101.2 days. CONCLUSION: Disability duration of the representative musculoskeletal diseases comparable to AIS code in 4th edition of Korean standard classification of diseases was presented and this study can be utilized as a basic recommended disability duration of automobile injured persons.
Abbreviated Injury Scale
;
Accidents, Traffic
;
Automobiles*
;
Classification
;
Dislocations
;
Humans
;
Insurance, Disability
;
Joints
;
Length of Stay
;
Lower Extremity
;
Musculoskeletal Diseases
;
Sprains and Strains