No abstract available.
Bacteriuria*

PURPOSE: To evaluate the cause of asymptomatic bacteriuria (AB) or aseptic pyuria (AP) on physiologic phimosis and to evaluate the effect of topical steroid therapy and preputial hygiene on the resolution of AB and AP. METHODS: Ninety uncircumcised boys (age 1-72 month, median 16 month) with AB or AP were examined for physiologic phimosis and allocated by the preputial retractibility into the non-retractile group (n=59) or the retractile group (n=31). Topical steroid therapy [topical application of hydrocortisone (0.1%) cream with physiotherapy] were prescribed (three times a day) and the method of preputial hygiene (gentle retraction of prepuce and water cleansing) was instructed to the non- retractile group . After 2-4 weeks, the preputial retractibility was reevaluated and urine examination was repeated. To the retractile group, only the method of preputial hygiene was instructed and urine examination was repeated two weeks later. RESULTS: Among 90 boys with AB and AP, 65.6% (59/90) had the nonretractile prepuces and nonperformed preputial hygiene. In the nonretractile group, the prepuces became retractile in 81.4 % (48/59) after topical steroid therapy. Among boys (n=48) whose prepuces became retractile after topical steroid therapy, AB or AP resolved in 77.1%, decreased in 18.7% and persisted in 4.2%, which were significantly different to 18.2%, 2.37%, 54.5% in boys (n=11) whose prepuces were persistently nonretractile (P=0.0114). In the retractile group (n=31), 65.2% was compliant to preputial hygiene. In boys (n=23) who were compliant to preputial hygiene, AB or AP resolved in 65.2%, decreased in 26.0% and persisted in 8.2%, which were significantly different to 12.5%, 50%, 37.5% in boys (n=8) who were not compliant (P=0.0457). CONCLUSION: Physiologic phimosis was an important cause of AB or AP. Simple topical steroid therapy on the nonretractile prepuces and good preputial hygiene could improve AB or AP.
Bacteriuria ; Female ; Hydrocortisone ; Hygiene ; Phimosis ; Pyuria ; Water

Of acute scrotal lesions in the neonates, cases needing emergent exploration are quite rare and differential diagnosis of them is not easy. Only a few cases of inflamed hydroceles occurring in young boys have been reported to have been caused by bacterial infection in the literature. We couldn't find any case concerning simultaneous bacterial infection in urine and hydrocele by the same organisms. We present an unusual case of infected hydrocele in a neonate with bacteriuria.
Bacteriuria/*etiology ; Case Report ; Human ; Hydrocele/*etiology ; Infant, Newborn ; Male

BACKGROUND: The purpose of this study was to discover ways to screen urine culture specimens through Gram stains, urine stick analyses and microscopic examinations for the laboratory cost saving. METHODS: One hundred and fifty-eight urine specimens for culture were included. Fifty uL of urine were inoculated onto one well each of 10-well slide, dried on the hot plate, and Gram-stained. The results combined with routine urinalyses including urine nitrite and leukocyte esterase, and pyuria, were compared with the routine culture results. RESULTS: The screening of bacteriuria by Gram stains, urinalyses and microscopic examinations revealed the high sensitivity (91.9%) and negative predictive value(95.5%) with cost saving of 41.8% of inoculating media. Not considering the Gram stains, the screening revealed 83.8% sensitivity and 92.5% negative predictive value, even if the cost saving of inoculating media were as high as 50.1%. CONCLUSION: It was demonstrated that it was sensitive and economic and produced rapid preliminary results to screen bacteriuria by the Gram stains combined with urinalyses and microscopic examinations.
Bacteriuria ; Coloring Agents ; Cost Savings ; Leukocytes ; Mass Screening* ; Pyuria ; Urinalysis*

BACKGROUND: Rapid detection of bacteriuria is desirable for diagnosis and treatment of urinary tract infections. The aim of this study was to assess the usefulness of the cytocentrifuge Gram stain, uncentrifuged Gram stain, and urinary nitrite, leukocyte esterase to determine the exclusion of urine culture. METHODS: A cytocentrifuge Gram stain procedure and urine dipstick test were performed to screen for bacteriuria using 155 random fresh urine specimens submitted for routine culture. The authors compared the results of the cytocentrifuge Gram stain, urinary nitrite, leukocyte esterase and multiapplication of three tests with the results of culture. Result: Compared with positive urine culture(>105CFU/mL), cytocentrifuge Gram stain had a good negative predictive value(96.0%) and sensitivity(89.1%). The urinary nitrite and leukocyte esterase test had very low sensitivity(27.0%, 45.9%), respectively. The multiapplication of cytocentrifuge Gram stain, urinary nitrite and leukocyte esterase had a excellent negative predictive value(98.9%) and sensitivity(97.3%), and agree with urine culture positive(36/37 cases, 97.3%). CONCLUSION: Multiapplication of urinary cytocentrifuge Gram stain, nitrite and leukocyte esterase test is a useful screening test for the rapid exclusion of bacteriuria and provides rapid morphologic information about suspected pathogens in cases with positive urine culture.
Bacteriuria* ; Diagnosis ; Leukocytes ; Mass Screening* ; Urinary Tract Infections

BACKGROUND: Although bacteriologic culture remains the gold standard for detecting bactriuria, leukocyte esterase, nitrite and urine microscopy are also widely used. However, it is not uncommon to be presented with a discrepancy between these tests. METHODS: We performed leukocyte esterase and nitrite tests by urine reagent strip testing (Combur 10 Test, Roche, Germany), microscopic urinalysis, and by bacteriologic urine culture on 290 persons. RESULTS: The number of pathogens and non-pathogens were 51 (17.6%) and 87 (30.0%) respectively. The number of cases in which no microorganism was isolated (NMI group) was 152 (52.4%). The concordance rate between leukocyte esterase and microscopic leukocytes (leukocyturia) was 70.7%. The sensitivities and specificities of leukocyte esterase, microscopic leukocyte and microscopic bacteria were 72.5%/61.8%, 76.5%/44.1 % and 80.4%/92.1% respectively, and their positive predictive values and negative predictive values were 24% (62.3%)/69.1% (89.7%), 21.3% (53.6%)/62.6% (88.8%) and 51.9% (84.8%)/66.4% (95.3%), respectively. Although the nitrite test showed higher positive rates in the pathogen groups (37.2%) than in the non-pathogen group (2.2%) and NMI group (1.3%), this was only marginally significant. CONCLUSION: The microscopic estimation of bacteria is more a predictable marker of bacteriuria, rather than leukocyte esterase, nitrite, and microscopic leukocytes, in urine.
Bacteria ; Bacteriuria* ; Humans ; Leukocytes* ; Microscopy ; Reagent Strips ; Urinalysis

Urinary tract infections frequently affect pregnant mothers. This problem causes significant morbidity and healthcare expenditure. Three common clinical manifestations of UTIs in pregnancy are: asymptomatic bacteriuria, acute cystitis and acute pyelonephritis. Escherichia coli remains the most frequent organism isolated in UTIs. All pregnant mothers should be screened for UTIs in pregnancy and antibiotics should be commenced without delay. Urine culture and sensitivity is the gold standard in diagnosing UTIs. Without treatment, asymptomatic bacteriuria in pregnancy is associated with preterm delivery, intrauterine growth retardation, low birth weight, maternal hypertension, pre-eclampsia and anaemia. Acute pyelonephritis can lead to maternal sepsis. Recurrent UTIs in pregnancy require prophylactic antibiotic treatment.
Pregnancy ; Urinary tract infection ; Asymptomatic bacteriuria ; Acute ; therapeutic aspects

BACKGROUND: Although bacteriologic culture remains the gold standard for detecting bactriuria, leukocyte esterase, nitrite and urine microscopy are also widely used. However, it is not uncommon to be presented with a discrepancy between these tests. METHODS: We performed leukocyte esterase and nitrite tests by urine reagent strip testing (Combur 10 Test, Roche, Germany), microscopic urinalysis, and by bacteriologic urine culture on 290 persons. RESULTS: The number of pathogens and non-pathogens were 51 (17.6%) and 87 (30.0%) respectively. The number of cases in which no microorganism was isolated (NMI group) was 152 (52.4%). The concordance rate between leukocyte esterase and microscopic leukocytes (leukocyturia) was 70.7%. The sensitivities and specificities of leukocyte esterase, microscopic leukocyte and microscopic bacteria were 72.5%/61.8%, 76.5%/44.1 % and 80.4%/92.1% respectively, and their positive predictive values and negative predictive values were 24% (62.3%)/69.1% (89.7%), 21.3% (53.6%)/62.6% (88.8%) and 51.9% (84.8%)/66.4% (95.3%), respectively. Although the nitrite test showed higher positive rates in the pathogen groups (37.2%) than in the non-pathogen group (2.2%) and NMI group (1.3%), this was only marginally significant. CONCLUSION: The microscopic estimation of bacteria is more a predictable marker of bacteriuria, rather than leukocyte esterase, nitrite, and microscopic leukocytes, in urine.
Bacteria ; Bacteriuria* ; Humans ; Leukocytes* ; Microscopy ; Reagent Strips ; Urinalysis

PURPOSE: The incidence and severity of catheter associated bacteriuria might be dependent on the catheterized duration and route, although this has not been clearly established yet. We evaluated the severity of urinary tract infection according to the duration of the indwelling urethral catheter and the effect of antibiotic therapy in rabbits. MATERIALS AND METHODS: A total of 54 rabbits were divided into one control group and two experimental groups; antibiotic and no antibiotic group, which were subdivided according to catheterized period(1, 4, 7 and 14-day). We examined the urinalysis, urine culture, qualitative biofilm culture and scanning electro-microscopy of the urethral catheter. RESULTS: 1. In the urinalysis, pyuria developed significantly both in 4, 7, 14-day group of no-antibiotic and in 14-day of antibiotic group compared to the control group(p<0.05). In the no antibiotic 7-day group, pyuria developed significantly as compared to that of the antibiotic group(p<0.05). 2. In the urine culture, positive urine culture was increased significantly in 7, 14-day of no-antibiotic group and 14-day of antibiotic group compared to the control group(p<0.05). In the no antibiotic 7-day group, positive urine culture developed significantly as compared to the antibiotic group(p<0.05). 3. In the qualitative biofilm culture of the urethral catheter, positive culture developed significantly in the no antibiotic 7, 14-day group and the antibiotic 14-day group as compared to the control group(p<0.05). Positive biofilm culture was developed significantly in the no antibiotic 7, 14-day group as compared to the antibiotic group(p<0.05). 4. In scanning electro-microscopy, bacterial biofilm of the urethral catheter was similar to the qualitative biofilm culture, and increased with increasing duration of the catheterization. CONCLUSIONS: It is suggested that the early removal of the urethral catheter is important for the reduction of chance of urethral catheter associated urinary tract infection. In case of a long-term indwelling urethral catheter(more than 2 weeks), prevention of urinary tract infection was very difficult despite antibiotic administration.
Bacteriuria ; Biofilms ; Catheterization ; Catheters ; Incidence ; Pyuria ; Rabbits ; Urinalysis ; Urinary Catheters* ; Urinary Tract Infections* ; Urinary Tract*

PURPOSE: Persistent pyuria is one of the common complications after transurethral prostatectomy (TURP). Postoperative pyuria has an effect on postoperative voiding symptoms. Thus, postoperative urinalysis and urine culture are reliable indicators when following up voiding symptoms. In our study, possible preoperative, intraoperative and postoperative factors influencing the development and duration of pyuria and bacteriuria after TURP were evaluated. MATERIALS AND METHODS: Between January 2004 and November 2005, 82 patients who underwent TURP due to benign prostatic hyperplasia (BPH) were evaluated. The risk factors of the duration of the postoperative pyuria were divided into preoperative, intraoperative postoperative, and evaluated the differences and correlations according to these risk factors. RESULTS: The average durations of pyuria of 26 and 23 patients under and over the age of 70 were 4.12+/-2.69 and 6.61+/-4.51 weeks, respectively (p= 0.03). The average duration of pyuria of 12 patients with no preoperative pyuria and 37 with preoperative pyuria were 4.97+/-3.12 and 6.25+/-5.55 weeks, respectively (p=0.04). Separating patients according to the resected volume of prostate, the average duration of pyuria of 16 and 33 patients with resected prostate volumes greater than and less than 7g were 3.56+/-2.16 and 6.12+/-4.20 weeks, respectively (p=0.03). The age and average duration of pyuria showed a positive correlation (p=0.031). CONCLISIONS: Significant differences were observed in the duration of pyuria according to age, preoperative pyuria and resected volume of prostate.
Bacteriuria ; Humans ; Prostate ; Prostatic Hyperplasia ; Pyuria* ; Risk Factors ; Transurethral Resection of Prostate* ; Urinalysis