1.Advances in the study of Mycobacterium tuberculosis protein phosphatase and its inhibitors.
Tie-Shan TENG ; Hong-Hai WANG ; Jian-Ping XIE
Acta Pharmaceutica Sinica 2011;46(12):1420-1428
Reversible protein phosphorylation regulates multiple biochemical events. Mycobacterium tuberculosis phosphatases play important roles in regulating the pathogen physiology and interference of host signaling. They are also involved in the evasion of host immune response and blockage of the phagosome-lysosome fusion. Selective inhibition of phosphatase represents an ideal new avenue of anti-tuberculosis drug design. In this paper, we update the progresses about the regulation network of Mycobacterium tuberculosis phosphatases including MptpA, MptpB, MstP, SapM and their inhibitors. These serve as the basis for further antituberculosis drug target.
Acid Phosphatase
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antagonists & inhibitors
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metabolism
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Animals
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Antitubercular Agents
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pharmacology
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Bacterial Proteins
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antagonists & inhibitors
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metabolism
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Humans
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Mycobacterium tuberculosis
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drug effects
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enzymology
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Protein Tyrosine Phosphatases
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antagonists & inhibitors
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metabolism
2.Regulatory genes of geldanamycin biosynthesis.
Weiqing HE ; Jian LEI ; Yuying LIU ; Yiguang WANG
Chinese Journal of Biotechnology 2008;24(5):717-722
Two LAL family regulatory genes, gdmRI and gdmRII, were identified in the geldanamycin biosynthetic gene cluster of Streptomyces hygroscopicus 17997. Disruption of the two regulatory genes resulted in absolute elimination of geldanamycin biosynthesis. The complementation experiments using a single wild-type gene could restore geldanamycin production. These results indicated that both gdmRI and gdmRII were positive regulatory genes of the geldanamycin biosynthesis.
Anti-Bacterial Agents
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biosynthesis
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Benzoquinones
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metabolism
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Gene Expression Regulation, Bacterial
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HSP90 Heat-Shock Proteins
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antagonists & inhibitors
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Lactams, Macrocyclic
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metabolism
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Protein-Tyrosine Kinases
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antagonists & inhibitors
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Repressor Proteins
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genetics
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Streptomyces
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genetics
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metabolism
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Trans-Activators
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genetics
3.Correlation of Ciprofloxacin Resistance with the AdeABC Efflux System in Acinetobacter baumannii Clinical Isolates.
Abdollah ARDEBILI ; Abdolaziz Rastegar LARI ; Malihe TALEBI
Annals of Laboratory Medicine 2014;34(6):433-438
BACKGROUND: Acinetobacter baumannii is one of the most important pathogens capable of colonization in burn patients, leading to drug-resistant wound infections. This study evaluated the distribution of the AdeABC efflux system genes and their relationship to ciprofloxacin resistance in A. baumannii isolates collected from burn patients. METHODS: A total of 68 A. baumannii clinical strains were isolated from patients hospitalized in Motahari Burns Center in Tehran, Iran. Ciprofloxacin susceptibility was tested by the disk diffusion and agar dilution methods. PCR amplification of the adeRS-adeB drug efflux genes was performed for all resistant and susceptible isolates. To assess the role of the drug efflux pump in ciprofloxacin susceptibility, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) was used as an efflux pump inhibitor (EPI). RESULTS: Approximately 95.6% of the Acinetobacter isolates were resistant to ciprofloxacin, with minimum inhibitory concentration (MIC) values ranging from 4 to > or =128 microg/mL. The susceptibility of 86.1% of the resistant isolates increased by factors of 2 to 64 in the presence of CCCP. All resistant isolates were positive for the adeRS-adeB genes, and 73.2% of them had mutations in the AdeRS regulatory system. CONCLUSIONS: The results showed that AdeABC genes are common in A. baumannii, which might be associated with ciprofloxacin non-susceptibility, as indicated by the observed linkage to the presence of the genes essential for the activity of the AdeABC, several single mutations occurring in the adeRS regulatory system, and an increase of ciprofloxacin susceptibility in the presence of a CCCP EPI.
ATP-Binding Cassette Transporters/antagonists & inhibitors/genetics/*metabolism
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Acinetobacter Infections/diagnosis/microbiology
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Acinetobacter baumannii/*drug effects/genetics/isolation & purification
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Anti-Bacterial Agents/*pharmacology
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Bacterial Proteins/antagonists & inhibitors/genetics/*metabolism
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Base Sequence
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Ciprofloxacin/*pharmacology
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DNA, Bacterial/chemistry/genetics/metabolism
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Drug Resistance, Bacterial
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Humans
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Hydrazones/pharmacology
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Microbial Sensitivity Tests
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Mutation
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Polymerase Chain Reaction
4.Protein targets for structure-based anti-Mycobacterium tuberculosis drug discovery.
Protein & Cell 2010;1(5):435-442
Mycobacterium tuberculosis, which belongs to the genus Mycobacterium, is the pathogenic agent for most tuberculosis (TB). As TB remains one of the most rampant infectious diseases, causing morbidity and death with emergence of multi-drug-resistant and extensively-drug-resistant forms, it is urgent to identify new drugs with novel targets to ensure future therapeutic success. In this regards, the structural genomics of M. tuberculosis provides important information to identify potential targets, perform biochemical assays, determine crystal structures in complex with potential inhibitor(s), reveal the key sites/residues for biological activity, and thus validate drug targets and discover novel drugs. In this review, we will discuss the recent progress on novel targets for structure-based anti-M. tuberculosis drug discovery.
Bacterial Proteins
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antagonists & inhibitors
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chemistry
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genetics
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metabolism
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Crystallography, X-Ray
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Drug Discovery
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Genomics
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Models, Molecular
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Molecular Targeted Therapy
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Mycobacterium tuberculosis
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drug effects
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genetics
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metabolism
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Protein Conformation
5.Novel inhibitors against the bacterial signal peptidase I.
Guo-Jian LIAO ; Ying HE ; Jian-Ping XIE
Acta Pharmaceutica Sinica 2012;47(12):1561-1566
New antibiotics with novel modes of action and structures are urgently needed to combat the emergence of multidrug-resistant bacteria. Bacterial signal peptidase I (SPase I) is an indispensable enzyme responsible for cleaving the signal peptide of preprotein to release the matured proteins. Increasing evidence suggests that SPase I plays a crucial role in bacterial pathogenesis by regulating the excretion of a variety of virulent factors, maturation of quorum sensing factor and the intrinsic resistance against beta-lactams. Recently, breakthrough has been achieved in the understanding of three-dimensional structure of SPase I as well as the mechanism of enzyme-inhibitors interaction. Three families of inhibitors are identified, i.e. signal peptide derivatives, beta-lactams and arylomycins. In this article, we summarize the recent advance in the study of structure, activity and structure-activity relationship of SPase I inhibitors.
Animals
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Anti-Bacterial Agents
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pharmacology
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Bacteria
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drug effects
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Escherichia coli
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drug effects
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Membrane Proteins
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antagonists & inhibitors
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metabolism
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Oligopeptides
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chemistry
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pharmacology
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Serine Endopeptidases
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metabolism
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Serine Proteinase Inhibitors
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chemistry
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pharmacology
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Structure-Activity Relationship
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beta-Lactams
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antagonists & inhibitors
6.The role of bacterial biofilm in persistent infections and control strategies.
International Journal of Oral Science 2011;3(2):66-73
Bacterial biofilms can be viewed as a specific type of persistent bacterial infection. After initial invasion, microbes can attach to living and non-living surfaces, such as prosthetics and indwelling medical devices, and form a biofilm composed of extracellular polysaccharides, proteins, and other components. In hosts, biofilm formation may trigger drug resistance and inflammation, resulting in persistent infections. The clinical aspects of biofilm formation and leading strategies for biofilm inhibitors will be discussed in this mini-review.
Adhesins, Bacterial
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drug effects
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physiology
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Aminoacyltransferases
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antagonists & inhibitors
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genetics
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Animals
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Antimicrobial Cationic Peptides
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genetics
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pharmacology
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Bacterial Infections
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microbiology
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surgery
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Bacterial Proteins
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antagonists & inhibitors
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genetics
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Biofilms
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drug effects
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growth & development
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Chronic Disease
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Cysteine Endopeptidases
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genetics
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Cysteine Proteinase Inhibitors
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pharmacology
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Humans
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Inflammation
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microbiology
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Quorum Sensing
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drug effects
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physiology
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Wound Infection
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microbiology
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surgery
7.Advances in the study of the microbial efflux pumps and its inhibitors development.
Quan-Xin LONG ; Pei-Fu ZHOU ; Zong-Hui WU ; Hong-Hai WANG ; Jian-Ping XIE
Acta Pharmaceutica Sinica 2008;43(11):1082-1088
Drug resistant bacteria is an increasingly urgent challenge to public health. Bacteria adaptation and extensive abuse of antibiotics contribute to this dilemma. Active efflux of antibiotics is employed by the bacteria to survive the antibiotic pressure. Efflux pump is one of the hot spots of current drug related studies and ideal targets for the improvement of treatment. The efflux pumps and related mechanisms of action, regulation of expression and methodologies were summarized. Comparative genomics analyses were employed to elucidate the underlying mechanisms of action and evolution of efflux pump as exemplified by the Mycobacterium in our lab, which is a crucial re-emerging threat to global public health. The pathway and state-of-art drug development of efflux pump related drugs are included too.
ATP-Binding Cassette Transporters
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antagonists & inhibitors
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drug effects
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physiology
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Anti-Bacterial Agents
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metabolism
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pharmacology
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Bacteria
;
metabolism
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Drug Resistance, Multiple, Bacterial
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drug effects
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genetics
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Ion Pumps
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antagonists & inhibitors
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drug effects
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physiology
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Membrane Transport Proteins
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drug effects
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physiology
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Multidrug Resistance-Associated Proteins
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drug effects
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physiology
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Mycobacterium
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metabolism
8.Isolation of a Klebsiella pneumoniae Isolate of Sequence Type 258 Producing KPC-2 Carbapenemase in Korea.
Kyoung Ho ROH ; Chang Kyu LEE ; Jang Wook SOHN ; Wonkeun SONG ; Dongeun YONG ; Kyungwon LEE
The Korean Journal of Laboratory Medicine 2011;31(4):298-301
Carbapenem-resistant Klebsiella pneumoniae isolates producing K. pneumoniae carbapenemases (KPC) were first reported in the USA in 2001, and since then, this infection has been reported in Europe, Israel, South America, and China. In Korea, the first KPC-2-producing K. pneumoniae sequence type (ST) 11 strain was detected in 2010. We report the case of a patient with a urinary tract infection caused by KPC-2-producing K. pneumoniae. This is the second report of a KPC-2-producing K. pneumoniae infection in Korea, but the multilocus sequence type was ST258. The KPC-2-producing isolate was resistant to all tested beta-lactams (including imipenem and meropenem), amikacin, tobramycin, ciprofloxacin, levofloxacin, and trimethoprim-sulfamethoxazole, but was susceptible to gentamicin, colistin, polymyxin B, and tigecycline. The KPC-2-producing isolate was negative to phenotypic extended-spectrum beta-lactamase (ESBL) and AmpC detection tests and positive to modified Hodge test and carbapenemase inhibition test with aminophenylboronic acid.
Aged
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Bacterial Proteins/antagonists & inhibitors/metabolism
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Carbapenems/pharmacology
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Drug Resistance, Bacterial/genetics
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Female
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Humans
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Klebsiella pneumoniae/drug effects/genetics/*isolation & purification
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Microbial Sensitivity Tests
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Republic of Korea
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Sequence Analysis, DNA
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Urinary Tract Infections/*diagnosis/microbiology
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beta-Lactamases/antagonists & inhibitors/biosynthesis/*genetics/metabolism
9.Tunicamycin enhances TRAIL-induced apoptosis by inhibition of cyclin D1 and the subsequent downregulation of survivin.
Hai Yan ZHANG ; Zhen Xian DU ; Bao Qin LIU ; Yan Yan GAO ; Xin MENG ; Yifu GUAN ; Wei Wei DENG ; Hua Qin WANG
Experimental & Molecular Medicine 2009;41(5):362-369
TNF-related apoptosis-inducing ligand (TRAIL) has been proposed as a promising cancer therapy that preferentially induces apoptosis in cancer cells, but not most normal tissues. However, many cancers are resistant to TRAIL by mechanisms that are poorly understood. In this study, we showed that tunicamycin, a naturally occurring antibiotic, was a potent enhancer of TRAIL-induced apoptosis through downregulation of survivin. The tunicamycin-mediated sensitization to TRAIL was efficiently reduced by forced expression of survivin, suggesting that the sensitization was mediated at least in part through inhibition of survivin expression. Tunicamycin also repressed expression of cyclin D1, a cell cycle regulator commonly overexpressed in thyroid carcinoma. Furthermore, silencing cyclin D1 by RNA interference reduced survivin expression and sensitized thyroid cancer cells to TRAIL; in contrast, forced expression of cyclin D1 attenuated tunicamycin-potentiated TRAIL-induced apoptosis via over-riding downregulation of survivin. Collectively, our results demonstrated that tunicamycin promoted TRAIL-induced apoptosis, at least in part, by inhibiting the expression of cyclin D1 and subsequent survivin. Of note, tunicamycin did not sensitize the differentiated thyroid epithelial cells to TRAIL-induced apoptosis. Thus, combined treatment with tunicamycin and TRAIL may offer an attractive strategy for safely treating resistant thyroid cancers.
Anti-Bacterial Agents/*pharmacology
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*Apoptosis
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Cell Line, Tumor
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Cyclin D1/*antagonists & inhibitors/metabolism
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*Down-Regulation
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Humans
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Microtubule-Associated Proteins/*genetics/metabolism
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TNF-Related Apoptosis-Inducing Ligand/*metabolism
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Tunicamycin/*pharmacology
10.Tunicamycin enhances TRAIL-induced apoptosis by inhibition of cyclin D1 and the subsequent downregulation of survivin.
Hai Yan ZHANG ; Zhen Xian DU ; Bao Qin LIU ; Yan Yan GAO ; Xin MENG ; Yifu GUAN ; Wei Wei DENG ; Hua Qin WANG
Experimental & Molecular Medicine 2009;41(5):362-369
TNF-related apoptosis-inducing ligand (TRAIL) has been proposed as a promising cancer therapy that preferentially induces apoptosis in cancer cells, but not most normal tissues. However, many cancers are resistant to TRAIL by mechanisms that are poorly understood. In this study, we showed that tunicamycin, a naturally occurring antibiotic, was a potent enhancer of TRAIL-induced apoptosis through downregulation of survivin. The tunicamycin-mediated sensitization to TRAIL was efficiently reduced by forced expression of survivin, suggesting that the sensitization was mediated at least in part through inhibition of survivin expression. Tunicamycin also repressed expression of cyclin D1, a cell cycle regulator commonly overexpressed in thyroid carcinoma. Furthermore, silencing cyclin D1 by RNA interference reduced survivin expression and sensitized thyroid cancer cells to TRAIL; in contrast, forced expression of cyclin D1 attenuated tunicamycin-potentiated TRAIL-induced apoptosis via over-riding downregulation of survivin. Collectively, our results demonstrated that tunicamycin promoted TRAIL-induced apoptosis, at least in part, by inhibiting the expression of cyclin D1 and subsequent survivin. Of note, tunicamycin did not sensitize the differentiated thyroid epithelial cells to TRAIL-induced apoptosis. Thus, combined treatment with tunicamycin and TRAIL may offer an attractive strategy for safely treating resistant thyroid cancers.
Anti-Bacterial Agents/*pharmacology
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*Apoptosis
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Cell Line, Tumor
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Cyclin D1/*antagonists & inhibitors/metabolism
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*Down-Regulation
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Humans
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Microtubule-Associated Proteins/*genetics/metabolism
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TNF-Related Apoptosis-Inducing Ligand/*metabolism
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Tunicamycin/*pharmacology