Outbreaks of tick-borne disease cases in Santa Catarina, Brazil are known, but the presence of the pathogen DNA has never been determined. In this study, the first survey of Anaplasma marginale, Babesia bigemina, and Babesia bovis DNA on blood samples of 33 cattle from an outbreak in Ponte Alta Municipality, Santa Catarina, Brazil, has been carried out. A multiplex PCR detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. The most prevalent agent was B. bigemina (63.6%) followed by A. marginale (60.6%). This is the first report of tick-borne disease pathogens obtained by DNA analysis in Southern Brazil.
Anaplasma marginale/genetics/isolation & purification ; Anaplasmosis/*epidemiology ; Animals ; Babesia/genetics/isolation & purification ; Babesiosis/*epidemiology ; Brazil/epidemiology ; Cattle ; DNA, Protozoan/blood/isolation & purification ; Disease Outbreaks/*veterinary ; Polymerase Chain Reaction/*veterinary

Attempts were made to isolate and identify Korean bovine Babesia parasite. Blood samples were collected from Holstein cows in Korea, and Babesia parasites were propagated in SCID mice with circulating bovine red blood cells for isolation. The isolate was then antigenically and genotypically compared with several Japanese isolates. The Korean parasite was found to be nearly identical to the Oshima strain isolated from Japanese cattle, which was recently designated as Babesia ovata oshimensis n. var. Haemaphysalis longicornis was the most probable tick species that transmited the parasite.
Animals ; Arthropod Vectors/parasitology ; Babesia bovis/genetics/*isolation & purification ; Babesiosis/parasitology ; Base Sequence ; Cattle/*parasitology ; Cattle Diseases/parasitology ; DNA, Protozoan/genetics ; DNA, Ribosomal/genetics ; Erythrocytes/parasitology ; Korea ; Mice ; *Mice, SCID ; Molecular Sequence Data ; Phylogeny ; Ticks/parasitology

Babesiosis is an emerging tick-borne disease in humans worldwide; however, little is known about the frequency of infection or prevalence of this disease in other parts of the world, excluding North America. In this study, we aimed to investigate Babesia microti infection frequency in a human population in Mongolia. One hundred blood samples were collected from stock farmers living in Khutul city of Selenge province, Mongolia. The sera and DNA from blood samples were evaluated for the presence of B. microti infection by using indirect fluorescent antibody (IFA) tests and PCR. The positive detection rates obtained using the IFA tests and PCR assays were 7% and 3%, respectively. This study is the first to detect of B. microti infections based on antibody seroprevalence or PCR assays for the presence of B. microti DNA in a Mongolian population.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; *Animal Husbandry ; Antibodies, Protozoan/*blood ; Babesia microti/genetics/*isolation & purification ; Babesiosis/diagnosis/*epidemiology/immunology/pathology ; Child ; Child, Preschool ; DNA, Protozoan/*blood ; Female ; Fluorescent Antibody Technique, Direct ; Humans ; Male ; Middle Aged ; Mongolia/epidemiology ; Polymerase Chain Reaction ; Seroepidemiologic Studies ; Young Adult