Objective: To describe screening situation and associated factors of early warning signals in psychopathology in kindergarten children, so as to provide reference for effective prevention and treatment strategies. Methods: From October to December 2021, a cross sectional study was conducted in kindergartens in Jinniu District of Chengdu. The age appropriate early warning signals were screened among 20 417 kindergarten children for developmental problems. Chi square test and Logistic regression were used for univariate and multivariate analysis of positive early warning signs screening, respectively. Results: The positive screening of early warning signals among 2 year old children was mainly distributed in the language domain (6.12%), personal social domain for 2.5 year old (1.70%) and 3 year old children (1.76%), and fine motor domain for 4 year old (2.04%) and 5 year old children (1.03%). Univariate and multivariate analysis showed that factors associated with positive screening of early warning signals included gender, family structure, history of diseases, electronic screen exposure >2 h/d, sleep duration at night, and age of children( P < 0.05). Conclusion It is necessary to carry out regular screening for early warning signals for psychopathology in kindergartens. Age appropriate health education for early childhood development should be carried out against nurses and kindergarten teaching staff. Similarly, more attention should be paid to healthy sleep and media usage to improve physical and mental wellness among preschool children.