1.Regulatory B Cells Are Inversely Associated with Disease Activity in Rheumatoid Arthritis.
Jinhyun KIM ; Hyun Ji LEE ; In Seol YOO ; Seong Wook KANG ; Jae Ho LEE
Yonsei Medical Journal 2014;55(5):1354-1358
PURPOSE: The function of regulatory B lymphocytes is known to be abnormal in inflammatory diseases. However, a recent study indicates that IL-10+ B cells seem to be expanded in rheumatoid arthritis (RA). Therefore, the state of IL-10+ B cells in the peripheral blood from RA patients and healthy controls were investigated. MATERIALS AND METHODS: CD19+ cells in peripheral blood mononuclear cells were purified from blood samples of RA patients and age and gender-matched healthy controls, and stimulated with CD40 ligand and CpG for 48 hours. Then, intracellular IL-10 in CD19+ cells was analyzed using flow cytometry. RESULTS: There was no significant difference in the proportion of IL-10+ B cells between 10 RA patients and 10 healthy controls (RA, 0.300+/-0.07 vs. healthy control 0.459+/-0.07, p=0.114). The proportion of induced IL-10+ B cells to total B cells in RA patients was significantly higher than those in controls (RA, 4.44+/-3.44% vs. healthy control 2.44+/-1.64%, p=0.033). However, the proportion of IL-10+ B cells to total B cells correlated negatively with disease activity in RA patients (r=-0.398, p=0.040). Erythrocyte sedimentation rate or C-reactive protein or medication was not associated with the proportion of IL-10+ B cells. CONCLUSION: The proportion of induced IL-10+ B cell increased in RA patients compared to healthy control, however, negatively correlated with disease activity in RA.
Adult
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Aged
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Antigens, CD19/metabolism
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Arthritis, Rheumatoid/blood/*immunology/pathology
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B-Lymphocytes, Regulatory/metabolism/*physiology
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Biological Markers/blood
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Female
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Humans
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Interleukin-10/metabolism
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Male
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Middle Aged
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Severity of Illness Index