1.The result of Arbuscular mycorrhizal (AM) fungi research in Mongolia “Shajin Khurh” of Bogdkhan Mountain
Mongolian Medical Sciences 2015;172(2):96-102
Research main purpose is to study spores of arbuscular mycorrhizal fungi (AMF) and to classify sporetypes from soil. Samples were collected from six diverse types of soil: larch forest, larch forest edge,mountain slope, wetlands, steppe and winter camp around “Shajin Khurh” of Bogdkhan mountain.The soil sample was taken from the six sites such as larch forest, larch forest edge, foot of mountain,washland, steppe, winter camp which are included to the natural various zone near “Shajin Khurkh”of Bogd Khan Mountain. By the survey the research team had found 26 species of spore to carry outsurvey on soil arbuscular mycorrhizal fungal (AMF) spore.The survey was carried out at the flora laboratory of “School of Biology” under the University of InnerMongolia. We had the following result when the research work was done according to the researchwork methodology. Including:26 species of arbuscular mycorrhizal fungal (AMF) spore were determined from soil sample from thesix sites near “Shajin Khurkh” of Bogd Khan Mountain, Acaulaspora 5 species, Diversispora 3 species,Glomus 15 species, Entrophospora 1 species, Scutellospora 2 species were separated (shown in table1). 16 species from Larch forest, 14 species from steppe, 12 species from washland, 11 species fromLarch forest edge, 8 species from Graizing grassland, 7 species from mountain foot were determined.Soil samples were taken from investigatory 6 points in variety environmental ranges such as larchforest, larch forest edge, mountain slope, wetlands, and steppe and winter camp around “Shajin Khurh”of Bogdkhan Mountain. Research work to study AMF in root was performed there. And colonizationrate of AMF in 34 species, 23 genera and 15 families.When the research work was done in according to method, there is following results. Hereto: versicular16.4% and hyphae 39.1% in root contained in mountain slope soil versicular 14.3% and hyphae54.4% in larch forest edge soil, versicular 8.1% and hyphae 61% in larch forest soil, versicular 24%and hyphaе 42.4% in steppe soil, versicular 21.3% and hyphae 50% wetlands soil, 6.1% and hyphae55.1% in winter camp soil. Versicular and hyphae rate contained in root is showed in following table.Studying AMF (Arbuscular mycorrhizal fungi) colonization in root is very important. Fungal symbiosesin root are detected by the research work. Root and fungal symbioses are beneficial mutual. Plantshave ability to adapt in various condition of ecology and AMF is very important factor for rehabilitationecology. Thus I did AMF in roots. Attached pictures of mycrrohiza in some roots used to the research(Pictureг 2).
2. HE RESULT OF ARBUSCULARMYCORRHIZAL (AM) FUNGI RESEARCH OF MONGOLIA
Mongolian Pharmacy and Pharmacology 2013;2(1):40-
Introduction: Arbuscularmycorrhizal (AM) fungi inhabit various ecosystems with a wide range of host plant species. However, as these funge are obligate symbionts with livings roots, the hosts play an important role in mycorrhizal development, spore formation and distribution of AM fungi. The communityof AM fungus species in the rhizospere may vary with host species (McGonigle and Fitter 1990). Whether the host can influence diversity of mycorrhizal fungi under controlled condition is an important issue as different researchers often use different test plants for studies on AM fungal diversity (Frank-Snyder et al. 2001; Helgason et al. 1998).Research main purpose is to study spores of arbuscularmycorrhizal fungi (AMF) and to classify spore types from soil.Materials and methods: Samples were collected from six diverse types of soil: larch forest, larch forest edge, mountain slope, wetlands, steppe and winter camp around “ShajinKhur” of Bogdkhanmountain.About 2 kg soil (2-20 cm) was collected from five randomly selected locations at each site. Soils at each sity were bulked, mixed and five replicate subsamples taken. Spores of AM fungi of each soil were extracted by wet- sieving from aliquots ((50 ml))and identified to species (Schenck and Perez 1988).Results: The soil sample was taken from the six sites such as Larch forest, Larch forest edge, Foot of mountain, washland, steppe, winter camp which are included to the natural various zone near “ShajinKhurkh” of Bogd Khan Mountain. By the survey the research team had found 26 species of spore to carry out survey on soil arbuscularmycorrhizal fungal (spore.The survey was carried out at the flora laboratory of “School of Biology” under the University of Inner Mongolia. We had the following result when the research work was done according to the research work methodology. Including:26 species of arbuscularmycorrhizal fungal (AMF) spore were determined from soil sample from the six sites near ShajinKhurkh of Bogd Khan Mountain, Acaulaspora 5species, Diversispora 3 species, Glomus 15 species, Entrophospora 1 species, Scutellospora 2 species. 16 species from Larch forest, 14 species from steppe, 12 species from washland, 11 species from Larch forest edge, 8 species from Graizing grassland, 7 species from mountain foot were determined. There is abundant spores of 4 AMF species such as Acaulaspora sp1, Acaulaspora sp3, Glomusambisporum, G.microaggregatum at 6 sites where the research sample was taken. Also there is abundant spore such as G.fasciculatum at larch forest, Glomusmosseae larch forest edge, Glomusclarum at washland, Glomus sp2 at graizing grassland, Entrophosporainfrenquens at steppe.Conclusion: The soil sample was taken from the six sites such as larch forest, larch forest edge, foot of mountain, washland, steppe, winter camp which are included to the natural various zone. In the result, 26 species, 5 genera of spore to carry out survey on soil arbuscularmycorrhizal fungal (AMF) spore were determined including Acaulaspora 5 species, Diversispora 3 species, Glomus15 species, Entrophospora 1 species, Scutellospora 2 species. There is abundant Glomus type spore.The research work is being implemented to continuedoing the AMF survey further.References1. Frank-Snyder M, Douds DD Jr, Galvez L, Phillips JG, Wagoner P, Drinkwater L, Morton B (2001) Diversity of communities of Arbuscularmycorrhiza (AM) fungi present in conventional versus low-input agricultural sites in eastern Pennsylvania, USA. Appl Soil Ecol 16:35-482. McGonigle TP, Fitter AH (1990) Ecological specificity of vesicular-arbuscularmycorrhizal associations. Mycol Res 94:120-1223. Schenck NC, Perez Y (1988) Manual for identification of versiculararbuscularmycorrhizal fungi, 2nd edn. INVAM. University of Florida, Gainesvill, Fla.4. Densmaa B, Bao Yu ying. Diversity of AM fungi and Soil microbial quantity in Shajinkhurh area of Mongolia Bogd khan Mountain. China, Huh hot, 2012.