No abstract available.
Autoradiography*

No abstract available.
Autoradiography* ; DNA* ; Ultraviolet Rays*

After UV irradiation to mouse epidermis, autoradiography using H-thymidine was performed to study the effects of UV on scheduled and unschcdu ed DNA syntheses. The results can be summarized as follows. l. In case of UVB 100mJ/cm, heavily labeled cells (HLCs) representing scheduled DNA synthesis began to decrease immediately after UVB irradiai,ior and were significantly decreased after 24 hours, but then recovered after 48 hours. The ecovery was maintained from 72 hours, up to 7 days. Sparsely labeled cells (SLCs) repres inting unscheduled DNA synthesis were significantly increased from immediately after UV 3 irradiation and up to 6 hours later. Repair was present at 24 hours, and was maintained at 48 hours, 72 hours, and 7 day after irradiation. 2. In case of UVA, UVA 10J/cm did not affect DNA synthesis sign ficantly. But in cases of UVA 30J/cm and 50J/cm, HLCs began to decrease immediately after UVA irradiation and were significantly decreased for 24 hours. Recovery occured at 48 hours, and was maintained from 72 hours up to 7 days. SLCs were significently increased immediately after and up to 6 hours after UVB irradiation. Repair generally occurred after 24 hours, and was maintained at 48 hours, 72 hours, and 7 days after ir rad ation.
Animals ; Autoradiography ; DNA* ; Epidermis ; Mice*

No abstract available.
Autoradiography* ; Brain* ; Rats, Wistar* ; Receptors, Muscarinic*

No abstract available.
Animals ; Autoradiography ; Carcinogenesis* ; Diethylnitrosamine ; gamma-Glutamyltransferase* ; Liver* ; Rats*

No abstract available.
Animals ; Autoradiography ; Carcinogenesis* ; Diethylnitrosamine ; gamma-Glutamyltransferase* ; Liver* ; Rats*

The mammalian amygdala comprises a heterogeneous complex of cytoarchitectonically, histochemically and connectionally distinct nuclei. To investigate the developmental changes and regional distributions of adrenoceptor binding sites in the adult and postnatal rat amygdala (postnatal days 0, 5, 10, 15, 20, 30), in vitro autoradiography was performed. Binding sites for the alpha1-adrenoceptor ligand [3H]-prazosin, the alpha2-adrenoceptor ligand [3H]-rauwolscine, and the beta-adrenoceptor ligand [125I]-iodocyanopindolol were visualized by the in vitro autoradiography, and anatomically localized by comparing the autoradiograms to Nissl- and acetylcholinesterase-stained sections. On toluidine blue- and acetylcholinesterase-stained sections of the amygdaloid complex of the rat, three major divisions can be distinguished: the cortical-like nuclear group, medial nuclear group, and central nuclear group. The basolateral nuclear group of the cortical-like nuclear group was divided into three subregions, the basal, the basolateral and the basomedial amygdaloid nucleus. Between the medial and the basolateral amygdaloid nucleus, the intercalated nuclei were observed. The highest number of alpha1-adrenoceptor binding sites was detected in the central and the lateral amygdaloid nucleus. The nuclei most strongly labeled by [3H]-rauwolscine were those in the medial part of the amygdaloid complex. The pattern of the beta-adrenoceptor binding was relatively diffuse, the medial amygdaloid nucleus was most strongly labeled among the amygdaloid nuclei. At the postnatal day 0, adrenergic receptor binding sites were only weakly labeled. The expression of a1-adrenoceptor binding was rapidly increased in the central amygdaloid nucleus at the postnatal day 5, and between the postnatal day 10 and 15, the concentration of bindig sites reached the adult levels. The expression of alpha2- and beta-adrenoceptor binding was increased in most amygdaloid nuclei at the postnatal day 10, and higher density was observed at the postnatal day 30. In the adult, the expression of adrenoceptor binding was relatively low in most nuclei when compared to postnatal day 30. These findings may provide evidence that alpha1-adrenoceptor is involved in regulating amygdaloid development and function more specifically than alpha2- and beta-adrenoceptor during postnatal development. These results indicate that the regional distributions of alpha1-, alpha2-, and beta-adrenoceptor show some differences from those of the other mammalian species reported.
Adult ; Amygdala* ; Animals ; Autoradiography ; Binding Sites ; Humans ; Rats* ; Receptors, Adrenergic*

Autoradiographic study was performed in order to know the distribution of exogenous C(14)-glucose by lung fluke, Paragonimus westermani, incubated in Tyrode medium containing 10 uCi/ml of labeled substance. After 1 hour incubation at 37C, microautoradiographs of this fluke showed that black grains derived from radioactive carbon were accumulated mainly in the parenchyme and subcuticular musculature. The muscular tissues such as oral sucker, pharynx and ventral sucker revaled considerable density of fine grains. Slight radioactivity was also observed in the regions of ovary, testes, vitelline follicles, eggs in uterus, intestinal ceca, and even in excretory bladder. Structures showing the least activity included the cuticle and uterine tubules of this fluke.
parasitology-helminth-trematoda- Paragonimus wertermani ; autoradiography ; biochemistry-glucose ; Tyrode ; glucose

Experimental brain tumor model is essential for the development of new therapeutic modalities of brain tumors and evaluation of efficacy of each therapeutic variety. Authors developed experimental rat brain tumor model with 9L and C6 cell line in Fischer rat using stereotactic method. We tried to determine the tumor occurrence rate, the ideal time for secondary experiment using this brain tumor model, and the duration between the onset of neurological signs and the time of expiration. We performed autoradiography for each cell line to evaluate the reliance of the tumor model. We could make good tumor model in all the cases of experiment and except to use it in another extension of experiment.
Animals ; Autoradiography ; Brain Neoplasms* ; Brain* ; Cell Line ; Gliosarcoma* ; Rats*

OBJECTIVES: Calcium salts are usually present in the center of all types of gallstones, including pure cholesterol stones. It has been postulated that precipitation of calcium salts might act as a nidus for gallstone formation. Our goal was to determine whether a calcium-binding protein was present in bile and whether this protein has any calcium-binding properties. METHODS: Calcium-binding moiety was obtained by addition of CaC12 into the gallbladder bile. Calcium-binding protein was identified by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The capacity to bind calcium was confirmed by autoradiography with 45Ca++. RESULTS: We identified a protein(M.W. Autoradiography ; Bile* ; Calcium ; Cholesterol ; Electrophoresis, Polyacrylamide Gel ; Gallbladder* ; Gallstones ; Humans ; Salts