1.Effect of needle knife on mTOR/Atg/ULK1/Beclin-1 axis and chondrocyte autophagy in rats with knee osteoarthritis.
Man LU ; Xiao-Shuang HUANG ; De-Hong MENG ; Qian CHEN ; Tao LI ; Zong-Bao WANG ; Yong-Hui YANG ; Kai GENG
Chinese Acupuncture & Moxibustion 2022;42(1):59-65
OBJECTIVE:
To observe the effect of needle knife on chondrocyte autophagy and expressions of autophagy-related protein and mammalian target of rapamycin (mTOR) in rats with knee osteoarthritis (KOA), and to explore the possible mechanism of needle knife for KOA.
METHODS:
A total of 42 SD rats were randomly divided into a normal group, a model group and a needle knife group, 14 rats in each group. Except for the normal group, the other two groups were injected with the mixture of papain and L-cysteine into the left hind knee joint to establish the KOA model. After modeling, the rats in the needle knife group were treated with needle knife at strip or nodule around the quadriceps femoris and medial and lateral collateral ligament on the affected side, once a week for 3 times (3 weeks). The changes of left knee circumference in each group were observed; the chondrocytes and ultrastructure of left knee joint were observed by HE staining and electron microscope; the mRNA and protein expressions of autophagy-related genes (Atg5, Atg12, Atg4a), Unc-51 like autophagy activated kinase 1 (ULK1), autophagy gene Beclin-1 and mTOR in left knee cartilage were detected by real-time fluorescence quantitative PCR and Western blot.
RESULTS:
After modeling, the left knee circumferences in the model group and the needle knife group were increased compared with those before modeling and in the normal group (P<0.05); after intervention, the left knee circumference in the needle knife group was smaller than that in the model group and after modeling (P<0.05). Compared with the normal group, the number of chondrocytes was decreased, and a few cells swelled, nuclei shrank, mitochondria swelled and autophagosomes decreased in the model group; compared with the model group, the number of chondrocytes was increased , and most cell structures returned to normal, and autophagosomes was increased. Compared with the normal group, the mRNA and protein expressions of Atg5, Atg12, Atg4a, Beclin-1 and ULK1 in the knee cartilage in the model group were decreased (P<0.05); compared with the model group, the expressions of the above indexes in the needle knife group were increased (P<0.05). Compared with the normal group, the mRNA and protein expressions of mTOR in the knee cartilage in the model group were increased (P<0.05); compared with the model group, the expressions of the above indexes in the needle knife group were decreased (P<0.05).
CONCLUSION
The needle knife intervention could improve knee cartilage injury in rats with KOA, and its mechanism may be related to reducing the expression of mTOR and up-regulating the expressions of Atg5, Atg12, Atg4a, ULK1 and Beclin-1, so as to promote chondrocyte autophagy and delay the aging and degeneration of chondrocytes.
Animals
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Autophagy
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Autophagy-Related Protein-1 Homolog/genetics*
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Beclin-1/genetics*
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Chondrocytes
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Osteoarthritis, Knee/therapy*
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Rats
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Rats, Sprague-Dawley
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TOR Serine-Threonine Kinases/genetics*
2.ULK1 and JNK are involved in mitophagy incurred by LRRK2 G2019S expression.
Yuangang ZHU ; Chunyan WANG ; Mei YU ; Jie CUI ; Liang LIU ; Zhiheng XU
Protein & Cell 2013;4(9):711-721
Mutations in LR RK2 (Leucine rich repeat kinase 2) are a major cause of Parkinson's disease (PD). We and others reported recently that expression of the pathogenic gainof-function mutant form of LRRK2, LRRK2 G2019S, induces mitochondrial fission in neurons through DLP1. Here we provide evidence that expression of LRRK2 G2019S stimulates mitochondria loss or mitophagy. We have characterized several LRRK2 interacting proteins and found that LRRK2 interacts with ULK1 which plays an essential role in autophagy. Knockdown of either ULK1 or DLP1 expression with shRNAs suppresses LRRK2 G2019S expression-induced mitochondrial clearance, suggesting that LRRK2 G2019S expression induces mitochondrial fission through DLP1 followed by mitophagy via an ULK1 dependent pathway. In addition to ULK1, we found that LRRK2 interacts with the endogenous MKK4/7, JIP3 and coordinates with them in the activation of JNK signaling. Interestingly, LRRK2 G2019S-induced loss of mitochondria can also be suppressed by 3 different JNK inhibitors, implying the involvement of the JNK pathway in the pathogenic mechanism of mutated LRRK2. Thus our findings may provide an insight into the complicated pathogenesis of PD as well as some clues to the development of novel therapeutic strategies.
Amino Acid Substitution
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Autophagosomes
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metabolism
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pathology
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Autophagy-Related Protein-1 Homolog
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chemistry
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genetics
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metabolism
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GTP Phosphohydrolases
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antagonists & inhibitors
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genetics
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metabolism
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Gene Knockdown Techniques
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HeLa Cells
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Humans
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Intracellular Signaling Peptides and Proteins
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chemistry
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genetics
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metabolism
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Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
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chemistry
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genetics
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metabolism
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MAP Kinase Signaling System
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Microtubule-Associated Proteins
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antagonists & inhibitors
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genetics
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metabolism
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Mitochondrial Degradation
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genetics
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physiology
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Mitochondrial Proteins
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antagonists & inhibitors
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genetics
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metabolism
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Mutant Proteins
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chemistry
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genetics
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metabolism
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Mutation
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Parkinson Disease
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genetics
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metabolism
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pathology
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Protein Interaction Domains and Motifs
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Recombinant Proteins
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chemistry
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genetics
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metabolism