1.A Systematic Investigation of Complement and Coagulation-Related Protein in Autism Spectrum Disorder Using Multiple Reaction Monitoring Technology.
Xueshan CAO ; Xiaoxiao TANG ; Chengyun FENG ; Jing LIN ; Huajie ZHANG ; Qiong LIU ; Qihong ZHENG ; Hongbin ZHUANG ; Xukun LIU ; Haiying LI ; Naseer Ullah KHAN ; Liming SHEN
Neuroscience Bulletin 2023;39(11):1623-1637
Autism spectrum disorder (ASD) is one of the common neurodevelopmental disorders in children. Its etiology and pathogenesis are poorly understood. Previous studies have suggested potential changes in the complement and coagulation pathways in individuals with ASD. In this study, using multiple reactions monitoring proteomic technology, 16 of the 33 proteins involved in this pathway were identified as differentially-expressed proteins in plasma between children with ASD and controls. Among them, CFHR3, C4BPB, C4BPA, CFH, C9, SERPIND1, C8A, F9, and F11 were found to be altered in the plasma of children with ASD for the first time. SERPIND1 expression was positively correlated with the CARS score. Using the machine learning method, we obtained a panel composed of 12 differentially-expressed proteins with diagnostic potential for ASD. We also reviewed the proteins changed in this pathway in the brain and blood of patients with ASD. The complement and coagulation pathways may be activated in the peripheral blood of children with ASD and play a key role in the pathogenesis of ASD.
Child
;
Humans
;
Autism Spectrum Disorder/metabolism*
;
Proteomics
;
Brain/metabolism*
2.The microbiota-gut-brain axis and neurodevelopmental disorders.
Qinwen WANG ; Qianyue YANG ; Xingyin LIU
Protein & Cell 2023;14(10):762-775
The gut microbiota has been found to interact with the brain through the microbiota-gut-brain axis, regulating various physiological processes. In recent years, the impacts of the gut microbiota on neurodevelopment through this axis have been increasingly appreciated. The gut microbiota is commonly considered to regulate neurodevelopment through three pathways, the immune pathway, the neuronal pathway, and the endocrine/systemic pathway, with overlaps and crosstalks in between. Accumulating studies have identified the role of the microbiota-gut-brain axis in neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, and Rett Syndrome. Numerous researchers have examined the physiological and pathophysiological mechanisms influenced by the gut microbiota in neurodevelopmental disorders (NDDs). This review aims to provide a comprehensive overview of advancements in research pertaining to the microbiota-gut-brain axis in NDDs. Furthermore, we analyzed both the current state of research progress and discuss future perspectives in this field.
Humans
;
Brain-Gut Axis
;
Autism Spectrum Disorder/metabolism*
;
Brain/metabolism*
;
Gastrointestinal Microbiome
;
Neurodevelopmental Disorders/metabolism*
3.WDR62-deficiency Causes Autism-like Behaviors Independent of Microcephaly in Mice.
Dan XU ; Yiqiang ZHI ; Xinyi LIU ; Le GUAN ; Jurui YU ; Dan ZHANG ; Weiya ZHANG ; Yaqing WANG ; Wucheng TAO ; Zhiheng XU
Neuroscience Bulletin 2023;39(9):1333-1347
Brain size abnormality is correlated with an increased frequency of autism spectrum disorder (ASD) in offspring. Genetic analysis indicates that heterozygous mutations of the WD repeat domain 62 (WDR62) are associated with ASD. However, biological evidence is still lacking. Our study showed that Wdr62 knockout (KO) led to reduced brain size with impaired learning and memory, as well as ASD-like behaviors in mice. Interestingly, Wdr62 Nex-cKO mice (depletion of WDR62 in differentiated neurons) had a largely normal brain size but with aberrant social interactions and repetitive behaviors. WDR62 regulated dendritic spinogenesis and excitatory synaptic transmission in cortical pyramidal neurons. Finally, we revealed that retinoic acid gavages significantly alleviated ASD-like behaviors in mice with WDR62 haploinsufficiency, probably by complementing the expression of ASD and synapse-related genes. Our findings provide a new perspective on the relationship between the microcephaly gene WDR62 and ASD etiology that will benefit clinical diagnosis and intervention of ASD.
Mice
;
Animals
;
Microcephaly/genetics*
;
Autistic Disorder/metabolism*
;
Autism Spectrum Disorder/metabolism*
;
Nerve Tissue Proteins/metabolism*
;
Brain/metabolism*
;
Mice, Knockout
;
Cell Cycle Proteins/metabolism*
4.Role of Microtubule-Associated Protein in Autism Spectrum Disorder.
Qiaoqiao CHANG ; Hua YANG ; Min WANG ; Hongen WEI ; Fengyun HU
Neuroscience Bulletin 2018;34(6):1119-1126
Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by deficits in social interaction and communication, along with repetitive and restrictive patterns of behaviors or interests. Normal brain development is crucial to behavior and cognition in adulthood. Abnormal brain development, such as synaptic and myelin dysfunction, is involved in the pathogenesis of ASD. Microtubules and microtubule-associated proteins (MAPs) are important in regulating the processes of brain development, including neuron production and synaptic formation, as well as myelination. Increasing evidence suggests that the level of MAPs are changed in autistic patients and mouse models of ASD. Here, we discuss the roles of MAPs.
Animals
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Autism Spectrum Disorder
;
metabolism
;
physiopathology
;
Autistic Disorder
;
metabolism
;
physiopathology
;
Disease Models, Animal
;
Humans
;
Microtubule-Associated Proteins
;
metabolism
;
Neurons
;
metabolism
;
Social Behavior
5.Identification of de novo Mutations in the Chinese Autism Spectrum Disorder Cohort via Whole-Exome Sequencing Unveils Brain Regions Implicated in Autism.
Bo YUAN ; Mengdi WANG ; Xinran WU ; Peipei CHENG ; Ran ZHANG ; Ran ZHANG ; Shunying YU ; Jie ZHANG ; Yasong DU ; Xiaoqun WANG ; Zilong QIU
Neuroscience Bulletin 2023;39(10):1469-1480
Autism spectrum disorder (ASD) is a highly heritable neurodevelopmental disorder characterized by deficits in social interactions and repetitive behaviors. Although hundreds of ASD risk genes, implicated in synaptic formation and transcriptional regulation, have been identified through human genetic studies, the East Asian ASD cohorts are still under-represented in genome-wide genetic studies. Here, we applied whole-exome sequencing to 369 ASD trios including probands and unaffected parents of Chinese origin. Using a joint-calling analytical pipeline based on GATK toolkits, we identified numerous de novo mutations including 55 high-impact variants and 165 moderate-impact variants, as well as de novo copy number variations containing known ASD-related genes. Importantly, combined with single-cell sequencing data from the developing human brain, we found that the expression of genes with de novo mutations was specifically enriched in the pre-, post-central gyrus (PRC, PC) and banks of the superior temporal (BST) regions in the human brain. By further analyzing the brain imaging data with ASD and healthy controls, we found that the gray volume of the right BST in ASD patients was significantly decreased compared to healthy controls, suggesting the potential structural deficits associated with ASD. Finally, we found a decrease in the seed-based functional connectivity between BST/PC/PRC and sensory areas, the insula, as well as the frontal lobes in ASD patients. This work indicated that combinatorial analysis with genome-wide screening, single-cell sequencing, and brain imaging data reveal the brain regions contributing to the etiology of ASD.
Humans
;
Autism Spectrum Disorder/metabolism*
;
Autistic Disorder
;
Exome Sequencing
;
DNA Copy Number Variations
;
East Asian People
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Brain/metabolism*
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Mutation/genetics*
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Genetic Predisposition to Disease/genetics*
6.Family-Based Association Study of Tryptophan-2,3 Dioxygenase (TDO2) Gene and Autism Spectrum Disorder in the Korean Population.
Soon Ae KIM ; Mi Ra PARK ; In Hee CHO ; Hee Jeong YOO
Journal of the Korean Academy of Child and Adolescent Psychiatry 2007;18(2):123-129
OBJECTIVES: Autism is a complex neurodevelopmental spectrum disorder with a strong genetic component. Previous neurochemical and genetic studies have suggested the possible involvement of the serotonin system in autism. Tryptophan 2,3-dioxygenase(TDO2) is the rate-limiting enzyme in the catabolism of tryptophan, which is the precursor of serotonin synthesis. The aim of this study was to investigate the association between the TDO2 gene and autism spectrum disorders(ASD) in a Korean population. METHODS: The patients were diagnosed with ASD on the basis of the DSM-IV diagnostic classification outlined in the Korean version of the Autism Diagnostic Interview-Revised and Autism Diagnostic Observation Schedule. The present study included the detection of four single nucleotide polymorphisms(SNPs) in the TDO2 gene(rs2292536, rs6856558, rs6830072, rs6830800) and the family-based association analysis of the single nucleotide polymorphisms in Korean ASD trios using a transmission disequilibrium test(TDT) and haplotype analysis. The family trios of 136 probands were included in analysis. 87.5% were male and 86.0% were diagnosed with autism. The mean age of the probands was 78.5+/-35.8 months(range: 26-264 months). RESULTS: Two SNPs showed no polymorphism, and there was no significant difference in transmission in the other two SNPs. We also could not find any significant transmission in the haplotype analysis(p>.05). CONCLUSION: We could not find any significant statistical association between the transmission of SNPs in the TDO2 gene and ASD in a Korean population. This result may not support the possible involvement of the TDO2 gene in the development of ASD, and further exploration might be needed to investigate other plausible SNP sites.
Appointments and Schedules
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Autistic Disorder*
;
Child
;
Autism Spectrum Disorder*
;
Classification
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Diagnostic and Statistical Manual of Mental Disorders
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Haplotypes
;
Humans
;
Male
;
Metabolism
;
Polymorphism, Single Nucleotide
;
Serotonin
;
Tryptophan
7.The Levels of Vitamin D, Vitamin D Receptor, Homocysteine and Complex B Vitamin in Children with Autism Spectrum Disorders.
Hatice ALTUN ; Ergül Belge KURUTAŞ ; Nilfer ŞAHIN ; Olcay GÜNGÖR ; Ebru FINDIKLI
Clinical Psychopharmacology and Neuroscience 2018;16(4):383-390
OBJECTIVE: Autism spectrum disorder (ASD) is a complex neurodevelopmental syndrome with an increasingly prevalent etiology, yet not fully understood. It has been thought that vitamin D, complex B vitamin levels and homocysteine are associated with environmental factors and are important in ASD. The aim of this study was to examine serum vitamin D, vitamin D receptor (VDR), homocysteine, vitamin B6, vitamin B12 and folate levels in ASD. METHODS: In this study, serum vitamin D and VDR, homocysteine, vitamins B6, B12 and folate levels were determined in 60 patients with ASD (aged 3 to 12 years) and in 45 age-gender matched healthy controls. In addition, calcium, phosphorus and alkaline phosphatase, which are associated with vitamin D metabolism, were measured from serum in both groups. ASD severity was evaluted by the Childhood Autism Rating Scale (CARS). RESULTS: Serum vitamin D and VDR were substantially reduced in patients with ASD in comparision to control group. However, homocysteine level was significantly higher and vitamin B6, vitamin B12 and folate were also reduced in patients with ASD. Total CARS score showed a positive association with homocysteine and a negative correlation with vitamins D, B6, B12, folate and VDR. CONCLUSION: This comprehensive study, which examines many parameters has shown that low serum levels of vitamins D, B6, B12, folate and VDR as well as high homocysteine are important in the etiopathogenesis of ASD. However, further studies are required to define the precise mechanism(s) of these parameters and their contributions to the etiology and treatment of ASD.
Alkaline Phosphatase
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Autism Spectrum Disorder*
;
Autistic Disorder*
;
Calcium
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Child*
;
Folic Acid
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Homocysteine
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Humans
;
Metabolism
;
Phosphorus
;
Receptors, Calcitriol*
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Vitamin B 12
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Vitamin B 6
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Vitamin D*
;
Vitamins*
8.RhoGEF Trio Regulates Radial Migration of Projection Neurons via Its Distinct Domains.
Chengwen WEI ; Mengwen SUN ; Xiaoxuan SUN ; Hu MENG ; Qiongwei LI ; Kai GAO ; Weihua YUE ; Lifang WANG ; Dai ZHANG ; Jun LI
Neuroscience Bulletin 2022;38(3):249-262
The radial migration of cortical pyramidal neurons (PNs) during corticogenesis is necessary for establishing a multilayered cerebral cortex. Neuronal migration defects are considered a critical etiology of neurodevelopmental disorders, including autism spectrum disorders (ASDs), schizophrenia, epilepsy, and intellectual disability (ID). TRIO is a high-risk candidate gene for ASDs and ID. However, its role in embryonic radial migration and the etiology of ASDs and ID are not fully understood. In this study, we found that the in vivo conditional knockout or in utero knockout of Trio in excitatory precursors in the neocortex caused aberrant polarity and halted the migration of late-born PNs. Further investigation of the underlying mechanism revealed that the interaction of the Trio N-terminal SH3 domain with Myosin X mediated the adherence of migrating neurons to radial glial fibers through regulating the membrane location of neuronal cadherin (N-cadherin). Also, independent or synergistic overexpression of RAC1 and RHOA showed different phenotypic recoveries of the abnormal neuronal migration by affecting the morphological transition and/or the glial fiber-dependent locomotion. Taken together, our findings clarify a novel mechanism of Trio in regulating N-cadherin cell surface expression via the interaction of Myosin X with its N-terminal SH3 domain. These results suggest the vital roles of the guanine nucleotide exchange factor 1 (GEF1) and GEF2 domains in regulating radial migration by activating their Rho GTPase effectors in both distinct and cooperative manners, which might be associated with the abnormal phenotypes in neurodevelopmental disorders.
Autism Spectrum Disorder/metabolism*
;
Cell Movement/genetics*
;
Humans
;
Interneurons/metabolism*
;
Neurodevelopmental Disorders/genetics*
;
Neurons/metabolism*
;
Rho Guanine Nucleotide Exchange Factors/genetics*
9.Analysis of inborn error metabolism in 277 children with autism spectrum disorders from Hainan.
Haijie SHI ; Jie WANG ; Zhendong ZHAO
Chinese Journal of Medical Genetics 2019;36(9):870-873
OBJECTIVE:
To assess the value of dry blood spot tandem mass spectrometry for the diagnosis of autism spectrum disorder (ASD).
METHODS:
Peripheral blood samples of 277 autistic children were collected. Their amino acid and carnitine profiles were detected by liquid chromatography tandem mass spectrometry. Urine samples of suspected patients were collected for verification by gas chromatography mass spectrometry. Blood samples were also taken for genetic testing.
RESULTS:
Of the 277 children with ASD, 19 (6.9%) were suspected to be with inborn error of metabolism (IEM), which included 6 cases with amino acidemia, 9 with organic acidemia and 4 with fatty acidemia. Three cases of phenylketonuria, one case of homocysteinemia, one case of propionemia, one case of methylmalonic acidemia, one case of glutaric acidemia, one case of isovaleric acidemia, one case of argininemia, one case of citrullinemia I and four cases of primary carnitine deficiency were confirmed by genetic testing, which yielded an overall diagnostic rate of 5.1% (14/277).
CONCLUSION
Our result has provided further evidence for the co-occurrence of ASD and IEM. Tandem mass spectrometry has a great value for the diagnosis and treatment of ASD in childhood.
Amino Acid Metabolism, Inborn Errors
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complications
;
diagnosis
;
Autism Spectrum Disorder
;
complications
;
diagnosis
;
Child
;
Dried Blood Spot Testing
;
Gas Chromatography-Mass Spectrometry
;
Humans
;
Metabolism, Inborn Errors
;
complications
;
diagnosis
;
Tandem Mass Spectrometry