1.Frequency of different subtypes of spinocerebellar ataxia in the Han nationality of Hunan province in China.
Xing-wang SONG ; Bei-sha TANG ; Hong JIANG ; Lu SHEN ; Qian YANG ; Shu-sheng LIAO ; Qing-hua LI ; Jian-guang TANG
Journal of Central South University(Medical Sciences) 2006;31(5):702-705
OBJECTIVE:
To determine the frequency of different subtypes of spinocerebellar ataxias (SCAs) in the Han nationality of Hunan province in China.
METHODS:
The mutations of SCA1, SCA2, SCA3, SCA6, SCA7, SCA17, and dentatorulral-pallidoluysian (DRPLA) were detected with the polymerase chain reaction (PCR), denaturing polyacrylamide gel and DNA sequencing techniques in 139 autosomal dominant SCA families and 61 sporadic SCA patients.
RESULTS:
Of the 139 families, 11 (7.9%) were positive for SCA1, 9(6.5%) were positive for SCA2, 71 (51.1%) were positive for SCA3, 4 (2.9%) were positive for SCA6, 2 (1.4%) were positive for SCA7, and none was positive for SCA17 and DRPLA. There was 1 SCA2 patient, 3 SCA3 patients, 1 SCA6 patient in the 61 sporadic SCA patients.
CONCLUSION
The frequency of SCA3 is substantially higher than that of SCA1 and SCA2 in the autosomal dominant SCA patients in the Han nationality of Hunan province. SCA6 and SCA7 are rare subtypes.
Adolescent
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Adult
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Ataxin-1
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Ataxin-3
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Ataxin-7
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Ataxins
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Child
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China
;
ethnology
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DNA Mutational Analysis
;
Female
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Humans
;
Male
;
Middle Aged
;
Nerve Tissue Proteins
;
genetics
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Nuclear Proteins
;
genetics
;
Repressor Proteins
;
genetics
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Spinocerebellar Ataxias
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classification
;
diagnosis
;
genetics
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Trinucleotide Repeats
;
genetics
3.Expressions of Sca-1, CD24 and Muc1 in quiescent mammary glands of female rats.
Jin-Quan CHEN ; Jun LIU ; Jing AN
Journal of Southern Medical University 2007;27(4):488-491
OBJECTIVETo investigate the expressions of the stem cell antigen-1 (Sca-1), Mucin1 (Muc1) and CD24 in quiescent mammary glands of female rats.
METHODSThe expressions of CD24 and Sca-1 were detected in 6- and 9-week-old female rat mammary gland by Western blotting. Sections (4 microm) of 6- and 9-week-old female SD rat mammary gland were prepared to observe the expressions of Sca-1, Muc1 and CD24 by immunohistochemical labeling and immunofluorescence labeling.
RESULTSCD24 and Sca-1 in the mammary glands were expressed at lower level in 6-week-old female rats than in 9-week-old female rats. Sca-1 expression was detected in the mammary gland ductus, branching ductus, and areas surrounding the gland alveolus; CD24 was expressed in the mammary gland branching ductus and fat pads, and also the regions surrounding the gland alveolus. Muc1 expression was localized in the mammary gland ductus and branching ductus.
CONCLUSIONSSca-1-, CD24- and Muc1-positive cells may represent mammary gland progenitor cells, mammary gland stem cells, and mammary gland mature epithelium cells, respectively. This study provides some morphological evidences for identifying these cells, but they still need further verifications in cellular transplantation experiments.
Animals ; Ataxin-1 ; Ataxins ; CD24 Antigen ; metabolism ; Female ; Gene Expression Profiling ; Mammary Glands, Animal ; metabolism ; Mucin-1 ; metabolism ; Nerve Tissue Proteins ; metabolism ; Nuclear Proteins ; metabolism ; Rats
4.Murine Sca1+Lin- bone marrow contains an endodermal precursor population that differentiates into hepatocytes.
Keunhee OH ; Suh Youn SHON ; Myung Won SEO ; Hak Mo LEE ; Ju Eun OH ; Eun Young CHOI ; Dong Sup LEE ; Kyong Soo PARK
Experimental & Molecular Medicine 2015;47(10):e187-
The direct differentiation of hepatocytes from bone marrow cells remains controversial. Several mechanisms, including transdifferentiation and cell fusion, have been proposed for this phenomenon, although direct visualization of the process and the underlying mechanisms have not been reported. In this study, we established an efficient in vitro culture method for differentiation of functioning hepatocytes from murine lineage-negative bone marrow cells. These cells reduced liver damage and incorporated into hepatic parenchyma in two independent hepatic injury models. Our simple and efficient in vitro protocol for endodermal precursor cell survival and expansion enabled us to identify these cells as existing in Sca1+ subpopulations of lineage-negative bone marrow cells. The endodermal precursor cells followed a sequential developmental pathway that included endodermal cells and hepatocyte precursor cells, which indicates that lineage-negative bone marrow cells contain more diverse multipotent stem cells than considered previously. The presence of equivalent endodermal precursor populations in human bone marrow would facilitate the development of these cells into an effective treatment modality for chronic liver diseases.
Animals
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Ataxin-1/*analysis
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Bone Marrow Cells/*cytology
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Cell Differentiation
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Cell Proliferation
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Cells, Cultured
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Female
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Hepatocytes/*cytology
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Mice
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Mice, Inbred BALB C
6.Analysis and application of SCA1 and SCA3/MJD gene CAG repeats in Han population in Northeastern China.
Miao JIANG ; Chun-lian JIN ; Chang-kun LIN ; Guang-rong QIU ; Zong-lan LIU ; Chao-xiang WANG ; Kai-lai SUN
Chinese Journal of Medical Genetics 2004;21(1):83-85
OBJECTIVETo investigate the normal range of (CAG)n in spinocerebellar ataxia type 1 (SCA1) gene and spinocerebellar ataxia type 3 (SCA3/MJD) gene in 110 normal subjects of Han population in Northeastern China, to assess the genotypes for clinically diagnosed spinocerebellar ataxia(SCA) individuals including 25 patients from 8 families and 6 sporadic patients, and to make presymptomatic and prenatal diagnosis.
METHODSDNA fragments from the normal subjects and the patients were detected by fluorescence-PCR. Homozygosities were selected for DNA sequencing.
RESULTSThe normal ranges of (CAG)n of SCA1 and SCA3/MJD were 20-39 and 14-38 repeats respectively, SCA1 was found mostly to be 26 and 27 repeats, allele frequency 34.09% and 20.91%; heterozygosity was 84.55%, SCA3/MJD was found mostly to be 14 repeats, allele frequency 39.55%, heterozygosity was 78.18%.(CAG)(68) of SCA3/MJD gene of one affected individual had been found in a family but no CAG mutative expansion in related members was observed.
CONCLUSIONThe normal ranges of CAG repeats vary with areas and races. SCAs genotyping is the first choice in presymptomatic and prenatal diagnosis.
Ataxin-1 ; Ataxin-3 ; Ataxins ; China ; DNA ; chemistry ; genetics ; Family Health ; Female ; Gene Frequency ; Genotype ; Humans ; Machado-Joseph Disease ; diagnosis ; genetics ; Male ; Nerve Tissue Proteins ; genetics ; Nuclear Proteins ; genetics ; Pedigree ; Repressor Proteins ; Sequence Analysis, DNA ; Spinocerebellar Ataxias ; diagnosis ; genetics ; Trinucleotide Repeat Expansion ; genetics ; Trinucleotide Repeats ; genetics
7.Construction of the eukaryotic expression vector of MJD1 and its expression in SH-SY5Y cells.
Jian-guang TANG ; Bei-sha TANG ; Lu SHEN ; Hong JIANG ; Zhi-ping HU ; Li CAO ; Kun XIA ; Fang CAI
Journal of Central South University(Medical Sciences) 2005;30(6):640-644
OBJECTIVE:
To construct the eukaryotic expression vector of MJD1 with normal copies of CAG trinucleotide repetition and MJD1 with CAG trinucleotide repetition expansion mutation respectively, and to determine whether the polyglutamine expansion in ataxin-3 could lead to the formation of intranuclear aggregation.
METHODS:
The coding sequence of wild-type MJD1 and mutant MJD1 was amplified by PCR from pAS2-1-MJD20Q and pAS2-1-MJD68Q respectively. After being digested with BamH I and Hind III, the PCR products were inserted into pcDNA3. 1-Myc-His(-) B. The recombinant plasmids pcDNA3.1-Myc-His(-) B-MJD20Q and pcDNA3.1-Myc-His(-) B-MJD68Q were identified by enzyme digestion analysis and DNA sequencing. The recombinant plasmid was transfected into SH-SYSY cells and the expression of MJD1 in the transfected cells was analyzed by Western blot. The immunofluorescence of the transfected cells was examined using a confocal microscope to observe the formation of intranuclear aggregation.
RESULTS:
Enzyme digestion analysis and DNA sequencing showed that the target gene was cloned into pcDNA3. 1-Myc-His(-) B. The expression of MJD1 in the transfected cells was confirmed by Western blot; The SH-SY5Y cells transfected with pcDNA3. 1-Myc-His(-) B-MJD68Q showed the formation of intranuclear aggregation, but the cells transfected with pcDNA3.1-Myc-His(-) B-MJD20Q did not show such phenomenon.
CONCLUSION
The eukaryotic expression vectors of MJD1 has been successfully constructed; The polyglutamine expansion in ataxin-3 could lead to the formation of intranuclear aggregation.
Ataxin-1
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Ataxin-3
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Ataxins
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Base Sequence
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Eukaryotic Cells
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metabolism
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Genetic Vectors
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Humans
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Mediator Complex
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Molecular Sequence Data
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Nerve Tissue Proteins
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biosynthesis
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genetics
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Neuroblastoma
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metabolism
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pathology
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Nuclear Proteins
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biosynthesis
;
genetics
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Plasmids
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genetics
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Receptors, Thyroid Hormone
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genetics
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Recombinant Proteins
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biosynthesis
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genetics
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Repressor Proteins
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biosynthesis
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genetics
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Transfection
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Tumor Cells, Cultured
8.Research on screening and identification of proteins interacting with ataxin-3.
Lu SHEN ; Jian-guang TANG ; Bei-sha TANG ; Hong JIANG ; Guo-hua ZHAO ; Kun XIA ; Yu-hu ZHANG ; Fang CAI ; Li-ming TAN ; Qian PAN
Chinese Journal of Medical Genetics 2005;22(3):242-247
OBJECTIVEThis study sought to isolate and identify the proteins that interact with ataxin-3, to confirm the interacted domain, and to provide new clues for exploring the function of ataxin-3 and the pathogenesis of spinocerebellar ataxia type 3 and Machado-Joseph disease (SCA3/MJD).
METHODSYeast two-hybrid screen (MATCHMAKER GAL4 Two-Hybrid System 3) and regular molecular biologic techniques were undertaken to screen human brain cDNA library with mutant ataxin-3 bait. Two baits from both normal and mutant C-terminus of ataxin-3 were created by subcloned methods to determine which domain of ataxin-3 interacts with the putative associated proteins and to find out optimal candidate proteins that interact with C-terminus of ataxin-3. Confocal microscope was used to observe whether ataxin-3 co-localized with the obtained interacting proteins in mammalian cells.
RESULTSFive novel ataxin-3 interacting proteins were obtained, among which were three known proteins, namely human rhodopsin guanosine diphosphate dissociation inhibitor alpha, small ubiquitin-like modifier 1, and human neuronal amiloride-sensitive cation channel 2; the other two were unknown. Interacting domain analysis revealed that an unknown protein interacted with the C-terminus near the polyglutamine tract of ataxin-3, the other four all interacted with the N-terminus. In the nucleus of SH-SY5Y cell, small ubiquitin-like modifier 1 co-localized with the wild-type ataxin-3 and with the intranuclear aggregates formed by the mutant ataxin-3.
CONCLUSIONAn unknown protein probably interacting with C-terminus of ataxin-3 is firstly discovered, and the initiative findings suggest first that the interaction of small ubiquitin-like modifier 1 with N-terminus of ataxin-3 and the relevant sumoylation probably participate in the post-translation modifying of ataxin-3 and in the pathogenesis of SCA3/MJD.
Acid Sensing Ion Channels ; Ataxin-3 ; Cell Line, Tumor ; Green Fluorescent Proteins ; genetics ; metabolism ; Humans ; Microscopy, Confocal ; Mutation ; Nerve Tissue Proteins ; genetics ; metabolism ; Nuclear Proteins ; genetics ; metabolism ; Plasmids ; genetics ; Protein Binding ; Recombinant Fusion Proteins ; genetics ; metabolism ; Repressor Proteins ; genetics ; metabolism ; SUMO-1 Protein ; genetics ; metabolism ; Sodium Channels ; genetics ; metabolism ; Transfection ; Two-Hybrid System Techniques
9.Expansive effects of aorta-gonad-mesonephros-derived stromal cells on hematopoietic stem cells from embryonic stem cells.
Jin-rong FU ; Wen-li LIU ; Yu-feng ZHOU ; Jian-feng ZHOU ; Han-ying SUN ; Li LUO ; Heng ZHANG ; Hui-zhen XU
Chinese Medical Journal 2005;118(23):1979-1986
BACKGROUNDHematopoietic stem cells (HSCs) give rise to all blood and immune cells and are used in clinical transplantation protocols to treat a wide variety of refractory diseases, but the amplification of HSCs has been difficult to achieve in vitro. In the present study, the expansive effects of aorta-gonad-mesonephros (AGM) region derived stromal cells on HSCs were explored, attempting to improve the efficiency of HSC transplantation in clinical practice.
METHODSThe murine stromal cells were isolated from the AGM region of 12 days postcoitum (dpc) murine embryos and bone marrow (BM) of 6 weeks old mice, respectively. After identification with flow cytometry and immunocytochemistry, the stromal cells were co-cultured with ESCs-derived, cytokines-induced HSCs. The maintenance and expansion of ESCs-derived HSCs were evaluated by detecting the population of CD34+ and CD34+Sca-1+ cells with flow cytometry and the blast colony-forming cells (BL-CFCs), high proliferative potential colony-forming cells (HPP-CFCs) by using semi-solid medium colonial culture. Finally, the homing and hematopoietic reconstruction abilities of HSCs were evaluated using a murine model of HSC transplantation in vivo.
RESULTSAGM and BM-derived stromal cells were morphologically and phenotypically similar, and had the features of stromal cells. When co-cultured with AGM or BM stromal cells, more primitive progenitor cells (HPP-CFCs) could be detected in ESCs derived hematopoietic precursor cells, but BL-CFC's expansion could be detected only when co-cultured with AGM-derived stromal cells. The population of CD34+ hematopoietic stem/progenitor cells were expanded 3 times, but no significant expansion in the population of CD34+Sca-1+ cells was noted when co-cultured with BM stromal cells. While both CD34+ hematopoietic stem/progenitor cells and CD34+Sca-1+ cells were expanded 4 to 5 times respectively when co-cultured with AGM stromal cells. AGM region-derived stromal cells, like BM-derived stromal cells, could promote hematopoietic reconstruction and HSCs' homing to BM in vivo.
CONCLUSIONSAGM-derived stromal cells in comparison with the BM-derived stromal cells could not only support the expansion of HSCs but also maintain the self-renewal and multi-lineage differentiation more effectively. They are promising in HSC transplantation.
Animals ; Antigens, CD34 ; analysis ; Aorta ; cytology ; Ataxin-1 ; Ataxins ; Bone Marrow Cells ; cytology ; physiology ; Cell Differentiation ; Cell Line ; Cell Lineage ; Embryo, Mammalian ; cytology ; Gonads ; cytology ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells ; cytology ; Male ; Mesonephros ; cytology ; Mice ; Mice, Inbred BALB C ; Nerve Tissue Proteins ; analysis ; Nuclear Proteins ; analysis ; Stromal Cells ; physiology