1.Effects of power frequency magnetic field on gap junction intercellular communication of astrocytes.
Xiu-zhen LI ; Suo-jiang ZHANG ; Ke-qing ZHU ; Xin ZHANG ; De-qiang LU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(2):132-134
OBJECTIVEIn order to explore if power frequency magnetic field (PFMF) can act as cancer promoter or be synergistic with phorbol 12-myristate 13-acetate (TPA) in cancer promotion, the effects of 50 Hz MF on gap junction intercellular communication (GJIC) of astrocytes were observed.
METHODSFluorescence redistribution after photobleaching (FRAP) was adopted to observe the recovery of fluorescence intensity in the bleached cells thus to estimate intercellular communication by gap junction. Comparative fluorescence intensity recovery rate (CFIRR) was as evaluation index. The effects of 50 Hz MF alone or with TPA on GJIC of astrocytes were studied.
RESULTSAfter 3 ng/ml TPA treatment for 1 hour, M(d) of CFIRR was 4.53%/min, whereas that in the control group was 9.74%/min (H = 12.084, P < 0.005). After exposure to 0.8 and 1.6 mT magnetic field for 24 hours respectively, M(d) of CFIRR was 8.25%/min and 6.68%/min respectively, no significant difference from that of control (H = 32.617, P > 0.05). After exposure to 0.8 and 1.6 mT magnetic field for 23 hours then combined with 3 ng/ml TPA treatment for 1 hour, M(d) of CFIRR was 3.32%/min and 2.85%/min respectively, also no significant difference from that in the group treated with 3 ng/ml TPA alone (H = 2.589, P > 0.05).
CONCLUSION50 Hz MF (within 0 - 1.6 mT) alone could not inhibit GJIC of astrocytes; with TPA, could not enhance the inhibition of TPA on GJIC of astrocytes. But with MF intensity increasing, the inhibition of MF on GJIC showed elevated tendency.
Animals ; Astrocytes ; radiation effects ; ultrastructure ; Cell Communication ; radiation effects ; Electromagnetic Fields ; adverse effects ; Gap Junctions ; radiation effects ; Ornithine Decarboxylase ; metabolism ; Rats ; Rats, Sprague-Dawley ; Tetradecanoylphorbol Acetate ; pharmacology
2.Gamma-ray irradiation stimulates the expression of caveolin-1 and GFAP in rat spinal cord: a study of immunoblot and immunohistochemistry.
Meejung AHN ; Heechul KIM ; Jeong Tae KIM ; Jeeyoung LEE ; Jin Won HYUN ; Jae Woo PARK ; Taekyun SHIN
Journal of Veterinary Science 2006;7(4):309-314
We studied the expression of caveolin-1 in the spinal cords of rats using 60Co gamma-ray irradiation (single dose of 8 Gray (Gy)) in order to determine the possible involvement of caveolin-1 in the tissues of the central nervous system after irradiation. Spinal cords sampled at days 1, 4, and 9 post-irradiation (PI) (n = 5 per each time point) were analyzed by Western blot and immunohistochemistry. Western blot analysis showed that the expression of caveolin-1 was significantly increased at day 1 PI (p < 0.05), and returned to the level of normal control rats on days 4 and 9 PI. Immunohistochemistry showed that caveolin-1 immunoreactivity was enhanced in some glial cells, vascular endothelial cells, and neurons in the spinal cords. The increased expression of glial fibrillary acidic protein (GFAP), a marker for an astroglial reaction, was consistent with that of caveolin-1. In addition, caveolin-1 was co-localized in hypertrophied GFAP-positive astrocytes. Taking all these facts into consideration, we postulate that irradiation induces the increased expression of caveolin-1 in cells of the central nervous system, and that its increased expression in astrocytes may contribute to hypertrophy of astrocytes in the spinal cord after irradiation. The precise role of caveolin-1 in the spinal cords should be studied further.
Animals
;
Astrocytes/metabolism/radiation effects
;
Blotting, Western
;
Caveolin 1/*biosynthesis
;
Gamma Rays
;
Glial Fibrillary Acidic Protein/*biosynthesis
;
Immunohistochemistry
;
Male
;
Rats
;
Rats, Sprague-Dawley
;
Spinal Cord/cytology/*metabolism/*radiation effects
;
Whole-Body Irradiation
3.Effect of Ketamine on Apoptosis by Energy Deprivation in Astroglioma Cells using Flow Cytometry System.
Soo Joo CHOI ; Myung Hee KIM ; Seung Woon LIM ; Mi Sook GWAK
Journal of Korean Medical Science 2005;20(1):113-120
Apoptosis is a programmed, physiologic mode of cell death that plays an important role in tissue homeostasis. As for the central nervous system, ischemic insults can induce pathophysiologic cascade of apoptosis in neurophils. Impairment of astroctye functions during brain ischemia can critically influence neuron survival by neuronglia interactions. We aimed to elucidate the protective effect of ketamine on apoptosis by energy deprivation in astrocytes. Ischemic insults was induced with iodoacetate/ carbonylcyanide mchlorophenylhydrazone (IAA/CCCP) 1.5 mM/ 20 micrometer or 150 micrometer/2 micrometer for 1 hr in the HTB-15 and CRL-1690 astrocytoma cells. Then these cells were reperfused with normal media or ketamine (0.1 mM) containing media for 1 hr or 24 hr. FITC-annexin-V staining and propidium iodide binding were determined by using flow cytometry. Cell size and granularity were measured by forward and side light scattering properties of flow cytometry system, respectively. An addition of keta-mine during reperfusion increased the proportion of viable cells. Ketamine alleviated cell shrinkage and increased granularity during the early period, and ameliorated cell swelling during the late reperfusion period. Ketamine may have a valuable effect on amelioration of early and late apoptosis in the astrocytoma cells, even though the exact mechanism remains to be verified.
Anesthetics, Dissociative/*pharmacology
;
Annexin A5/pharmacology
;
Apoptosis
;
Astrocytes/metabolism
;
Astrocytoma/*drug therapy/pathology
;
Brain/pathology
;
Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology
;
Cell Line, Tumor
;
Cell Size
;
Cell Survival
;
Central Nervous System/drug effects/pathology
;
Enzyme Inhibitors/pharmacology
;
Flow Cytometry/*methods
;
Humans
;
Indicators and Reagents/pharmacology
;
Iodoacetates/pharmacology
;
Ischemia/pathology
;
Ketamine/metabolism/*pharmacology
;
Light
;
Neurons/metabolism/pathology
;
Neutrophils/metabolism
;
Perfusion
;
Propidium/pharmacology
;
Scattering, Radiation
;
Time Factors
;
Uncoupling Agents/pharmacology