1.Cloning and characterization of 5'-upstream region of human phospholipase C-beta2 gene.
Eun Sook YUN ; Seung Jae LEE ; Myung Jong KIM ; Sung Ho RYU ; Pann Ghill SUH
Experimental & Molecular Medicine 2001;33(2):76-82
5'-upstream region of the phospholipase C-beta2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5-upstream region lacks both TATA motif and typical initiator sequence, but retains GC-rich segment. Two putative regulatory regions, a negative region (-636/-588) and a positive region (-98/ -13) were identified in the upstream region of PLC-beta2 gene. We suggest that the transcription of PLC-beta2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the gene.
Aspergillus Nuclease S1/metabolism
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Base Sequence
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Cells, Cultured
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Chloramphenicol O-Acetyltransferase/metabolism
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Cloning, Molecular
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Conserved Sequence
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Gene Deletion
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Isoenzymes/*chemistry/*genetics
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Phospholipase C/*chemistry/*genetics
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Promoter Regions (Genetics)
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Protein Binding
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Support, Non-U.S. Gov't
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Transcription, Genetic
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Transfection