3.Analysis of polymorphisms of mitochondrial DNA in 3 ethnic groups of Guizhou.
Ling-yan REN ; Yan HE ; Ting ZHANG ; Chan-juan WANG ; Zhi-zhong GUAN ; Keren SHAN
Chinese Journal of Medical Genetics 2013;30(5):626-631
OBJECTIVETo analyze the population genetics characteristics of mitochondrial DNA (mtDNA) in Gelao, Mulao, Maonan ethnic groups from Guizhou.
METHODSMinisequenceing and restriction fragment length polymorphism (RFLP) were used to analyze 12 single nucleotide polymorphism (SNPs) of mitochondrial DNA in the 3 ethnic groups.
RESULTSA total of 30 haplotypes were detected in 156 samples. The distribution of H1, H23 had differed between Mulao, Maonan and Gelao, respectively, and so did M7 among the three groups. The difference was statistically significant (P < 0.05). Mulao, Maonan had respectively differed from Gelao and the difference was also statistically significant (P < 0.05).
CONCLUSIONThere was a great similarity in the distribution of haplotypes of the mtDNA among the three ethnic groups, except for some difference in the distribution of certain haplotypes.
Asian Continental Ancestry Group ; ethnology ; genetics ; China ; ethnology ; DNA, Mitochondrial ; genetics ; Humans ; Male ; Pedigree ; Polymorphism, Genetic
4.Polymorphism of fifteen short tandem repeat loci in Tibetan of Changdu area.
Long-li KANG ; Yue-ya LI ; Xiao-feng ZHANG ; Feng-cang ZHAO ; Jian-min ZHAO ; Li-feng MA ; Xue HE ; Sheng-bin LI
Chinese Journal of Medical Genetics 2009;26(4):469-473
OBJECTIVETo investigate the genetic polymorphism of 15 autosomal short tandem repeat (STR) loci, i.e. D5S818, FGA, D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX and D18S51, in Tibetan population of Changdu area, and to analyze the genetic relationship between this population and other Asian populations.
METHODSThe 15 loci were amplified simultaneously using a multiplex PCR typing system. Amplified STR fragments were analyzed with an ABI PRISM 3100 Avant DNA Analyzer. Arlequin software version 3.1 was used to obtain allele frequencies and polymorphism parameters.
RESULTSThere were 135 STR alleles in the 15 STRs of Changdu Tibetan, with allele frequencies ranging from 0.0065 to 0.5455. The average heterozygosity was 0.7340, the discrimination power was higher than 0.8 except for TPOX and TH01. The accumulative discrimination power was more than 0.9999998, and the accumulative non-parental exclusion probability was 0.99999997.
CONCLUSIONThe 15 STR loci of Changdu Tibetan possess characteristics of high genetic diversity. They can be employed in minority genetics investigation, and individual and paternity test in forensic medicine.
Asian Continental Ancestry Group ; ethnology ; genetics ; Female ; Humans ; Male ; Microsatellite Repeats ; Pedigree ; Polymorphism, Genetic ; Tibet ; ethnology
5.Genetic polymorphisms of fifteen short tandem repeat loci in Guilin Han population.
Chinese Journal of Medical Genetics 2009;26(4):465-468
OBJECTIVETo investigate the genetic polymorphisms of 15 short tandem repeat (STRs) in Guilin Han population.
METHODSDNA was extracted by Chelex method, and 15 STRs were analyzed using AmpFISTR Identifiler kit.
RESULTSFour rare alleles, namely FGA * 10, D2S1338 * 10, D3S1358 * 16.2 and D3S1358 * 17.2, were observed. The combined match probability and exclusion probability for the 15 STRs were 2.89 x 10(-17) and 0.9999993, respectively.
CONCLUSIONThese STRs have good discrimination power and exclusion probability in Guilin Han population.
Asian Continental Ancestry Group ; ethnology ; genetics ; China ; ethnology ; Female ; Humans ; Male ; Microsatellite Repeats ; Polymorphism, Genetic
6.Investigation of the characteristics of Rh blood group of Uygur nationality in Xinjiang.
Jiong-Cai LAN ; Hua-You ZHOU ; Xu-Hua BAI ; Xiao-Ping CHEN ; Yan-Zhao XING
Journal of Experimental Hematology 2007;15(4):885-887
The study was to investigate the characteristics of Rh blood group of Uygur nationality in Xinjiang. 1 230 blood samples of Uygur nationality were studied by Rh serological typing, modified antiglobulin test, chloroform/trichloroethylene absorption elution test, upstream, downstream and hybrid Rhesus boxes, 10 exons of D gene, RHD(psi) pseudogene. The results showed that the frequency of RHD negative was 5.8%, and no Del type was found. The further investigation of 72 samples of RhD (-) found that ccee (57.02%) and Ccee (29.08%) phenotype as well as RHD(-)/RHD(-) genotype (94.44%) and complete deletion type of D gene exon (91.12%) were all in high frequency, no RHD(psi) pseudugene was detected. In conclusion, the Rh blood group of Uygurs nationality in Xinjiang possesses both oriental and caucasian Rh characteristics, which enriches the diversity of blood types in chinesenation.
Asian Continental Ancestry Group
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genetics
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China
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ethnology
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Gene Deletion
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Humans
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Rh-Hr Blood-Group System
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genetics
7.Methods for English translation of TCM terms of diseases and symptoms.
Chinese Journal of Integrated Traditional and Western Medicine 2006;26(9):855-857
TCM terms of diseases and symptoms are important elements of traditional Chinese medical nomenclature. Good English translation of these terms plays an active role in standardizing English translation of TCM terms. Through comparing the names of diseases and symptoms in Chinese and Western medicine and sorting, the author put forward in this paper several methods for translating TCM terms into English.
Asian Continental Ancestry Group
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China
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Disease
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ethnology
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Humans
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Medicine, Chinese Traditional
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Terminology as Topic
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Translations
8.Single nucleotide polymorphisms of growth hormone receptor gene in Chinese Han ethnic population.
Jing ZHOU ; Ying LU ; Yu-xing BAI ; Yan-ping WANG ; Yan SHEN ; Bang-kang WANG
Chinese Journal of Stomatology 2004;39(2):97-99
OBJECTIVETo analyze the distribution of single nucleotide polymorphisms (SNP) of growth hormone receptor (GHR) gene in Chinese Han ethnic population.
METHODSThe sample of 106 unrelated healthy Chinese Hans was studied by sequencing exons of the GHR gene to detect SNP.
RESULTSThere were 6 SNP spots identified in exon 6 and exon 10. Five of them were found in exon 10, and one in exon 6. There were differences between the allele frequencies of the SNP we found and those in the NCBI database. The highest heterozygosity of the SNP was found at 1630 A > C (I526L), which was 47.6%. The SNP 1483 A > C (P477T), 1735 A > C (P561T) and 1319 G > T (C422F) had polarity change. The SNP 536 A > G in exon 6 from the NCBI database was not detected in this population. The allele distribution of SNP was in good unity with the Hardey-Weinberg equilibrium.
CONCLUSIONIt is suggested that the SNP of GHR are unevenly distributed and different in different ethnic populations.
Asian Continental Ancestry Group ; ethnology ; Female ; Gene Frequency ; Humans ; Male ; Polymorphism, Single Nucleotide ; Receptors, Somatotropin ; genetics
9.Genetic polymorphisms of Penta D and E loci in Korean racial Chinese of Yanbian area in China.
Qing-song XU ; Yong-ji ZHANG ; Hong CUI ; Yan CUI
Chinese Journal of Medical Genetics 2006;23(2):234-235
OBJECTIVETo analyze genetic polymorphisms of Penta D and E loci in Korean racial Chinese of Yanbian area.
METHODSOne hundred unrelated individuals of Korean racial Chinese were analyzed by methods of PCR and restriction fragment length polymorphism.
RESULTSEight alleles and 22 genotypes were observed from Penata D locus, and 16 alleles and 35 genotypes were observed from Penta E locus.
CONCLUSIONThe distribution of two locus genotypes in Korean racial Chinese of Yanbian area met Hardy-Weinberg equilibrium. And the obtained data can be used to individual identity, paternity testing and the study of Korean ethnic group of Chinese population.
Asian Continental Ancestry Group ; genetics ; China ; ethnology ; Ethnic Groups ; genetics ; Humans ; Polymorphism, Genetic
10.Vasectomy does not increase the risk of prostate cancer in Chinese men: A meta-analysis.
Wen-qing LIAN ; Fei LUO ; Peng-liang CHEN ; Shen-fan WANG ; Bo-wei ZHOU ; Shan-chao ZHAO
National Journal of Andrology 2015;21(8):742-746
OBJECTIVETo investigate the correlation of vasectomy with the risk of prostate cancer in Chinese men.
METHODSWe systematically searched the databases CNKI, VIP, Wanfang, PubMed, Embase, and Cochrane Library for the literature relating the relationship between vasectomy and the risk of prostate cancer in Chinese males up to December 2014. According to the inclusion and exclusion criteria, two investigators independently selected the eligible publications, evaluated their quality, and extracted relevant information, followed by a meta-analysis with the software STATA 12.0.
RESULTSNine studies were included in the analysis involving 1 202 cases of prostate cancer and 4,496 controls. Random-effect model analysis revealed no statistically significant correlation between vasectomy and the risk of prostate cancer (OR = 1.05; 95% CI 0.62-1.79), with an obvious heterogeneity (P < 0.001, I2 = 85.7%). No significant publication bias was found among the included studies (Egger, P = 0.824; Begg, P = 0.348).
CONCLUSIONThe results of our meta-analysis do not support the association of vasectomy with the increased risk of prostate cancer in Chinese population.
Asian Continental Ancestry Group ; China ; Humans ; Male ; Prostatic Neoplasms ; ethnology ; etiology ; Risk Assessment ; Vasectomy ; adverse effects