No abstract available.
Ascitic Fluid* ; Ferritins*

No abstract available.
Ascitic Fluid ; T-Lymphocytes, Regulatory

No abstract available.
Ascitic Fluid* ; Endometriosis* ; Female ; Humans ; Interleukins*

Ascitic Fluid ; Consensus ; Humans ; Pleural Effusion

BACKGROUND: Inoculation of ascitic fluid into blood culture bottles is known to be more efficient than conventional culture method to diagnose spontaneous bacterial peritonitis. The aim of this study is to evaluate recovery and early detection of peritonitis-causing bacteria from ascitic fluid by using the BACTEC blood culture system with bedside inoculation. The results were compared to those of conventional culture method. METHODS: Ascitic fluid specimens from 345 patients suspicious of spontaneous bacterial peritonitis were prospectively evaluated between January 1999 and March 2002. In all cases, 5 to 10 mL of ascitic fluid were inoculated at the bedside into a pair of BACTEC blood culture bottles (BC method), and simultaneously an aliquot of ascitic fluid was sent to microbiology laboratory for conventional culture. Isolated microorganisms and the time elapsed for final report were compared between the two methods. RESULTS: BC method was positive in 66/345 ascitic fluid specimens (19.1%) and conventional culture method in 48/345 (13.9%) (P=0.065). Time elapsed for final report was 82 20.5 hours for blood culture method and 107 42.4 hours for conventional culture method (P=0.002). CONCLUSION: BC method using BACTEC system provides an earlier microbiologic diagnosis of spontaneous bacterial peritonitis than conventional culture method with higher sensitivity.
Ascitic Fluid* ; Bacteria ; Diagnosis ; Humans ; Peritonitis* ; Prospective Studies*

Ovarian primary malignant melanoma is very uncommon with only 44 reported cases in the literature. A 71-year-old woman with an ovarian mass and multiple nodules in the liver presented to our hospital. She was treated with bilateral salpingo-oophorectomy, and malignant melanoma was found in the mature cystic teratoma of the ovary. Malignant melanoma cells were also found in the ascitic fluid. She died 5 months later. Here we report a very uncommon case of malignant melanoma arising in an ovarian mature cystic teratoma with a review of the literature.
Aged ; Ascitic Fluid ; Female ; Humans ; Liver ; Melanoma ; Ovary ; Teratoma

BACKGROUND: Assessment of body fluid cytology is a useful means of evaluating a metastatic tumor. Liquid-based cytology (LBC) has been developed as a replacement for the conventional Papanicolaou (CP) test. This study was performed to compare CellprepPlus(R) LBC with CP in cytologic diagnosis. METHODS: Body fluid samples (n=188, including 72 peritoneal fluid and 116 pleural fluid samples) were divided equally and analyzed by both CellprepPlus(R) and CP. RESULTS: CellprepPlus(R) revealed distributed thin layers of non-overlapping cells. All CellprepPlus(R) preparations were adequate, while 18 (9.57%) CP preparations were inadequate. The respective diagnostic rates of CellprepPlus(R) and CP were 75.0% and 76.1% negative, 10.6% and 6.38% atypical, 5.85% and 2.66% suspicious, and 8.51% and 5.32% malignant. Of the 58 confirmed cases, the sensitivity of CellprepPlus(R) and CP was 94.4% and 73.3%, respectively, and the negative predictive value was 97.2% and 87.9%, respectively. CONCLUSIONS: CellprepPlus(R) LBC has better sensitivity and negative predictive value, and produces higher quality slide preparations than than CP, making it suitable as in screening of body fluid as a cytologic diagnostic tool.
Ascitic Fluid ; Body Fluids ; Mass Screening ; Neoplasm Metastasis

Meigs' sydrome is a relatively uncommon and the unusual condition of ascites and fluid in the chest associated with benign and solid tumors of the ovary with the gross appearance of a fibroma. The mechanism of this syndrome is not completely understood, but various lymphatics through the diaphragm seem the likely route for ascitic fluid into the chest. We present a case of Meigs' syndrome associated with fibrothecoma and elevate CA 125 with the brief review of literature.
Ascites ; Ascitic Fluid ; Diaphragm ; Female ; Fibroma ; Meigs Syndrome ; Ovary ; Thorax

No abstract available.
Animals ; Ascitic Fluid* ; Intestinal Obstruction* ; Lactic Acid* ; Rats*

Detecting malignant cells in ascitic fluid from tumor patients is important since the existence of malignant cells in ascitic fluids is related to the prognosis of patients. Various laboratory methods are being used to obtain diagnosis in ascitic fluids, but some ascitic fluids can not be diagnosed reliably. Cytogenetic analysis of ascitic fluid is not used routinely as a laboratory tool. In this presentation a cytogenetic study of the ascitic fluids from 9 patients with malignant tumor was performed by a direct or short-term culture method. According to cytogenetic study, 5 cases had positive findings for malignant cells. One case had a inconclusive result. There were no malignant cells in the remaining 4 cases. On blind cytologic data, no informations could be obtained in 4 out of 9 cases and the remaining 5 cases had negative findings for detecting malignant cells. Among the 5 cases, cytogenetic findings were negative in 3 cases but in the remaining 2 cases, one was reported positive and the other inconclusive each other. In present study, even though the ascitic fluids from 5 patients were subjected to the comparison of the cytologic study with cytogenetic analysis, two different findings could be obtained. Therefore if further study of a large series of cancer patients with ascitic fluids is done, the value of cytogenetic analysis will be clearly shown. In addition, the cytogenetic study of cell present in ascitic fluids can be used as useful adjunct to cytologic study, and also it can indicate that more invasive diagnostic procedures are necessary.
Ascitic Fluid* ; Cytogenetic Analysis ; Cytogenetics ; Diagnosis ; Humans ; Prognosis