1.Effects of heme oxygenase-1 upregulation on isoproterenol-induced myocardial infarction
Somaia A G ELTOBSHY ; Abdelaziz M HUSSEIN ; Asaad A ELMILEEGY ; Mona H ASKAR ; Yomna KHATER ; Emile F METIAS ; Ghada M HELAL
The Korean Journal of Physiology and Pharmacology 2019;23(3):203-217
The present study was designed to examine the effect of heme oxygenase-1 (HO-1) induction by cobalt protoporphyrin (CoPP) on the cardiac functions and morphology, electrocardiogram (ECG) changes, myocardial antioxidants (superoxide dismutase [SOD] and glutathione [GSH]), and expression of heat shock protein (Hsp) 70 and connexin 43 (Cx-43) in myocardial muscles in isoproterenol (ISO) induced myocardial infarction (MI). Thirty two adult male Sprague Dawely rats were divided into 4 groups (each 8 rats): normal control (NC) group, ISO group: received ISO at dose of 150 mg/kg body weight intraperitoneally (i.p.) for 2 successive days; ISO + Trizma group: received (ISO) and Trizma (solvent of CoPP) at dose of 5 mg/kg i.p. injection 2 days before injection of ISO, with ISO at day 0 and at day 2 after ISO injections; and ISO + CoPP group: received ISO and CoPP at a dose of 5 mg/kg dissolved in Trizma i.p. injection as Trizma. We found that, administration of ISO caused significant increase in heart rate, corrected QT interval, ST segment, cardiac enzymes (lactate dehydrogenase, creatine kinase-muscle/brain), cardiac HO-1, Hsp70 with significant attenuation in myocardial GSH, SOD, and Cx-43. On the other hand, administration of CoPP caused significant improvement in ECG parameters, cardiac enzymes, cardiac morphology; antioxidants induced by ISO with significant increase in HO-1, Cx-43, and Hsp70 expression in myocardium. In conclusions, we concluded that induction of HO-1 by CoPP ameliorates ISO-induced myocardial injury, which might be due to up-regulation of Hsp70 and gap junction protein (Cx-43).
Adult
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Animals
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Antioxidants
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Body Weight
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Cobalt
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Connexin 43
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Connexins
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Creatine
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Electrocardiography
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Glutathione
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Hand
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Heart Rate
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Heat-Shock Proteins
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Heme Oxygenase-1
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Heme
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HSP70 Heat-Shock Proteins
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Humans
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Isoproterenol
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Male
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Muscles
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Myocardial Infarction
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Myocardium
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Oxidoreductases
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Rats
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Tromethamine
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Up-Regulation