To investigate the temporal progression of atherogenesis on the aorta and involvement of the monocyte-macrophage system in the liver and spleen, we fed 74 rabbits with high fat (14 or 7 gm+ACU-) and cholesterol (2 and 1+ACU-) diets for 4 to over 24 weeks. Using both light and electron microscopies, we found that the bro-fatty areas on the luminal surface of aortas was spread over along the eding time dependently. The fat deposits also in the liver and spleen worsened pending on the time of feeding the atherogenic diets. Not only nocyte-derived foam cells, but also parenchymatous cells in the liver and leen involved become fat-laden cells. According to these results, we propose at there are three stages: 1) the primary seeding, 2) the intermediate turing and 3) the advanced periods. These periods may play very important les in designing the management and treatment of atherosclerotic patients.
Animal ; Aorta/pathology ; Aorta/drug effects+ACo- ; Aortic Diseases/pathology+ACo- ; Aortic Diseases/etiology ; Arteriosclerosis/pathology+ACo- ; Arteriosclerosis/etiology ; Cholesterol, Dietary/toxicity ; Diet, Atherogenic+ACo- ; Dietary Fats/toxicity+ACo- ; Dose-Response Relationship, Drug ; Fatty Liver/pathology+ACo- ; Fatty Liver/etiology ; Female ; Liver/pathology ; Liver/drug effects+ACo- ; Male ; Microscopy, Electron ; Rabbits ; Spleen/pathology ; Spleen/drug effects+ACo- ; Splenic Diseases/pathology+ACo- ; Splenic Diseases/etiology ; Time Factors

To investigate the temporal progression of atherogenesis on the aorta and involvement of the monocyte-macrophage system in the liver and spleen, we fed 74 rabbits with high fat (14 or 7 gm+ACU-) and cholesterol (2 and 1+ACU-) diets for 4 to over 24 weeks. Using both light and electron microscopies, we found that the bro-fatty areas on the luminal surface of aortas was spread over along the eding time dependently. The fat deposits also in the liver and spleen worsened pending on the time of feeding the atherogenic diets. Not only nocyte-derived foam cells, but also parenchymatous cells in the liver and leen involved become fat-laden cells. According to these results, we propose at there are three stages: 1) the primary seeding, 2) the intermediate turing and 3) the advanced periods. These periods may play very important les in designing the management and treatment of atherosclerotic patients.
Animal ; Aorta/pathology ; Aorta/drug effects+ACo- ; Aortic Diseases/pathology+ACo- ; Aortic Diseases/etiology ; Arteriosclerosis/pathology+ACo- ; Arteriosclerosis/etiology ; Cholesterol, Dietary/toxicity ; Diet, Atherogenic+ACo- ; Dietary Fats/toxicity+ACo- ; Dose-Response Relationship, Drug ; Fatty Liver/pathology+ACo- ; Fatty Liver/etiology ; Female ; Liver/pathology ; Liver/drug effects+ACo- ; Male ; Microscopy, Electron ; Rabbits ; Spleen/pathology ; Spleen/drug effects+ACo- ; Splenic Diseases/pathology+ACo- ; Splenic Diseases/etiology ; Time Factors

BACKGROUND: Inflammation and activation of immune cells have important roles in the pathogenesis of atherosclerosis. We analyzed the involvement of various immune cells in the pathogenesis of atherosclerosis. METHODS: We investigated the presence of foam cells, lymphocytes and killer cells in 11 atherosclerotic plaque specimens removed from Korean patients who underwent carotid endoarterectomy. Atherosclerotic plaques were analyzed by immunohistochemistry using monoclonal antibody specific to foam cells (anti-CD68), pan-T cells (anti-CD3), helper-T cells (anti-CD4), cytotoxic T cells (anti-CD8), granular component of killer cells (anti-TIA-1) and pan-B cells (anti-CD20). RESULTS: Analysis revealed a general infiltration of immune cells not only in atherosclerotic plaques but also in the vascular wall adjacent to the plaque. Heavy infiltration of CD68 macrophage was observed in all cases. In addition, significant infiltration of CD3 T-lymphocytes was observed in all cases, while CD20 B-cells were observed in only a few cases. Majority of the CD3 cells was found to be CD4 helper-T cells. CD8 cytotoxic T cells and TIA-1 cells were less prominent. CONCLUSION: Analysis of the human atherosclerotic plaques suggested that helper-T cells and foam cells had a major role in the plaque development.
Aged ; Aged, 80 and over ; Antigens, CD/analysis ; Antigens, Differentiation, Myelomonocytic/analysis ; Arteriosclerosis/pathology+ACo- ; Carotid Artery Diseases/pathology+ACo- ; Female ; Foam Cells/pathology+ACo- ; Human ; Male ; Middle Age ; Prevalence ; T-Lymphocytes, Helper-Inducer/pathology+ACo-