Research by …..method combined with block spectrum. High content of Artemisia ketone is in essential oil of Bungaria, almost no have in essential oil of Viet Nam. In bloom period, essential oil content of Artemisia annual L in Viet Nam only attained 0,1-0,2%, meanwhile Bungari’s attained 0,5-0,8% (reckon follow fresh weight). That showed Artermisia annual essential oil of Viet Nam no has significant meaning in economic and reality.
Artemisia ; Chemistry ; Oils, Volatile

OBJECTIVETo develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae.

METHODTLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (7:2.5:2.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (15:85) as mobile phase. The detection wavelength was 327 nm.

RESULTThe qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods.

CONCLUSIONThe methods and data could be used for quality control.

Artemisia ; chemistry ; Chlorogenic Acid ; analysis ; Plants, Medicinal ; chemistry ; Quality Control

OBJECTIVETo investigate the chemical constituents in flower buds of Artemisia scoparia.

METHODThe constituents were separated and purified by means of various chromatographic techniques, and their structures were determined on the basis of physical and chemical properties and spectral data.

RESULTFour flavones were isolated and their structures were identified as cirsilineol (I), cirsimaritin(II), arcapillin(III) and cirsiliol(IV) respectively.

CONCLUSIONCompounds I, III and IV were isolated from this plant for the first time.

Artemisia ; chemistry ; classification ; Flavones ; Flavonoids ; chemistry ; isolation & purification ; Flowers ; chemistry ; Plant Leaves ; chemistry ; Plants, Medicinal ; chemistry

To clarify the content characteristics of mineral elements in different Artemisia argyi germplasm resources and their relationship with the quality properties of Artemisiae Argyi Folium, this study measured the content of 10 mineral elements including nitrogen(N), phosphorus(P), potassium(K), calcium(Ca), magnesium(Mg), aluminum(Al), manganese(Mn), iron(Fe), copper(Cu), and zinc(Zn) in 100 Artemisia argyi germplasm samples. Besides, their relationship with the quality properties of Artemisiae Argyi Folium was explored by correlation analysis, path analysis, and cluster analysis. The results demonstrated that the variation coefficient of the 10 mineral elements in Artemisiae Argyi Folium ranged from 12.23% to 64.38%, and the genetic diversity index from 0.97 to 3.09. The genetic diversities of N, P, and Zn were obvious. As revealed by the correlation analysis, N, P, and K showed strong positive correlations with each other. Except that Mg and Al were negatively correlated, Ca, Mg, Al, Mn, Fe, Cu, and Zn were positively correlated. The correlation analysis of mineral elements with the quality properties of Artemisiae Argyi Folium proved the significant correlations of 17 pairs of characters. According to the path analysis, P, K, Ca, and Mn greatly affected the yield of Artemisiae Argyi Folium, P, K, and Mg the output rate of moxa, N, P, and K the content of total volatile oil, P and K the content of eucalyptol, and P, K, and Ca the content of eupatilin. The 100 germplasm samples were clustered into three groups. Specifically, in cluster Ⅰ, the enrichment capacity of P, K, and Mg elements was strong, and the comprehensive properties of mineral elements were better, implying good development potential. Ca, Mn, Fe, and Zn elements in cluster Ⅱ and N and Al in cluster Ⅲ displayed strong enrichment capacities. This study has provided new ideas for resource evaluation and variety breeding of A. argyi and also reference for fertilizer application.
Artemisia/genetics* ; Iron ; Minerals/analysis* ; Plant Breeding ; Plant Leaves/chemistry*

OBJECTIVETo study the chemical constituents of Artemisia rupestris.

METHODThe chemical constituents were isolated by column chromatography on silical gel and sephadex LH - 20. Their structures were elucidated on the basis of spectral analysis.

RESULT8 compounds have isolated from this plant, and the structures of them have identified as rupestonic acid (1), chrysosplenetin B (2), artemetin (3), herniarin (4), isokaempferide (5), vanillic acid (6), kaempferol 3, 3', 4'-trimethyl ether (7) and ermanine (8).

CONCLUSIONCompounds 2-8 have been isolated from this plant for the first time.

Artemisia ; chemistry ; Flavonoids ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Umbelliferones ; chemistry ; isolation & purification

OBJECTIVETo develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae.

METHODThe sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution (2.5:21.5:76) and detection wavelength was 347 nm.

RESULTThe arcapillin, cirsiliol, cirsimaritin and cirsilineol in bud extract were well separated by this method. Linearities of arcapillin, cirsiliol, cirsimaritin and cirsilineol were good (r = 0.9992, 0.9999, 0.9997, 0.9997) in ranges of 0.035-0.56, 0.043-0.69, 0.040-0.64 and 0.040-0.64 microg, respectively. The average recoveries were 96.8%, 97.3%, 96.8%, 98.5% and RSD the values of repeatability were 2.5%, 3.0%, 1.5%, 3.2%, respectively.

CONCLUSIONThe method validation data indicated that the method is reliable and it can be used for quality control of Herba Artemisiae Scopariae and its preparations.

Artemisia ; chemistry ; Chromatography, High Pressure Liquid ; Flavones ; analysis ; Flowers ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control

AIM: Artemisia amygdalina Decne. (Asteraceae) is a critically endangered and endemic herb of Kashmir Himalayan sub-alpine region and Pakistan. Scientific research throughout the world has evidence to support the tremendous medicinal utility of the genus Artemisia. The natural resources of medicinal plants are being reduced day by day. This study provides the alternative way for medicinal resource utilization and conservation of A. amygdalina. METHODS: In vitro-raised plants and greenhouse acclimatized plants were obtained by culturing wild explants on Murashige and Skoog's medium. Plant extracts were obtained and subjected to different antioxidant assays: DPPH assay, riboflavin photo-oxidation assay, deoxy ribose assay, ferric thiocyanate assay, thiobarbituric acid assay, post mitochondrial supernatant assay and DNA damage on agarose gel. RESULTS: In vitro grown plants, as well as those acclimatized in the greenhouse reveals antioxidant activity against hydroxyl, superoxide, and lipid peroxyl radicals. CONCLUSION This preliminary study revealed the free radical scavenging potential of tissue culture-raised plant extracts of A. amydalina.
Acclimatization ; Artemisia ; chemistry ; growth & development ; physiology ; Free Radical Scavengers ; chemistry ; Plant Extracts ; chemistry ; Tissue Culture Techniques

OBJECTIVETo study the chemical constituents of Artemisia ordosica.

METHODThe chemical constituents were isolated and repeatedly purified on silica gel column and the structures were elucidated by the NMR spectra and physico-chemical properties.

RESULTEight flavonoids were obtained and identified as 5-hydroxy-7, 4'-dimethoxyflavanone, 5-hydroxy-7, 4'-dimethoxyflavone, 5, 4'-dihydroxy-7-methoxyflavone, 5, 7-dihydroxy-6, 4'-dimethoxyflavone, 5, 4'-dihydroxy-7-methoxyflavanone, 5, 3', 4'-trihydroxy-7-methoxyflavanone, 5, 7-dihydroxy-3', 4'-dimethoxyflavone and 5, 3', 4'-trihydroxy-7-methoxyflavone.

CONCLUSIONAll the compounds were obtained from A. ordosica for the first time.

Apigenin ; chemistry ; isolation & purification ; Artemisia ; chemistry ; Flavones ; chemistry ; isolation & purification ; Flavonoids ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry

OBJECTIVETo investigate the chemical constituents in flower bud of Artemisia scoparia.

METHODThe constituents were isolated and purified by means of various chromatographic methods, and spectroscopic methods were used to identify their structures.

RESULTFour flavones were isolated and their structures were identified as hyperin (V), eupafolin (VI), pedalitin (VII), 5,7,2',4'-tetrahydroxy-6,5'-dimethoxyflavone (VIII).

CONCLUSIONCompounds V, VI, VII, VIII were obtained from this plant for the first time.

Artemisia ; chemistry ; classification ; Flavones ; chemistry ; isolation & purification ; Flowers ; chemistry ; Plants, Medicinal ; chemistry ; Quercetin ; analogs & derivatives ; chemistry ; isolation & purification

OBJECTIVETo study the chemical constituents of Artemisia rupestris.

METHODThe chemical constituents were isolated by column chromatography on silica gel, polyamide and Sephadex LH-20. Their structures were elucidated on the basis of spectral analysis.

RESULT11 compounds have isolated from this plant, and the structure of them have identified as robinin (1), quercetin (2), linearin (3), luteolin (4), sucrose (5), tilianin (6), luteolin-7-glucoside (7), beta-D-3'-methoxy-4'-O-P-coumaroylglucoside (8), hirsutine (9), 3-(4-methoxy phenyl)-(E)-2-propenoic acid ethyl ester (10), rutin (11).

CONCLUSIONAll this compounds have been isolated from this plant for the first time.

Artemisia ; chemistry ; Chromatography, Gel ; Flavonoids ; chemistry ; isolation & purification ; Luteolin ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Quercetin ; chemistry ; isolation & purification