1.Identification of Major and Minor Allergens of Mud Crab (Scylla Serrata)
Nurul Izzah Ar ; Rosmilah M ; Zailatul Hani My ; Noormalin A ; Faizal B ; Shahnaz M
Medicine and Health 2015;10(2):90-97
Crab meat is a valuable source of proteins and functional lipids and it is widely
consumed worldwide. However, the prevalence of crab allergy has increased
over the past few years. In order to understand crab allergy better, it is necessary
to identify crab allergens. The aim of the present study was to compare the IgEbinding
proteins of raw and cooked extracts of mud crab (Scylla serrata). Raw
and cooked extracts of the mud crab were prepared. Protein profiles and IgE
reactivity patterns were identified by sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE) followed by immunoblotting using sera from 21 skin
prick test (SPT) positive patients. In SDS-PAGE, 20 protein bands (12 to 250 kDa)
were observed in the raw extract while the cooked extract demonstrated fewer
bands. Protein bands between 40 to 250 kDa were sensitive to heat denaturation
and no longer observed in the cooked extract. In immunoblotting experiments,
raw and cooked extracts demonstrated 11 and 4 IgE-binding proteins, respectively,
with molecular weights of between 23 and 250 kDa. A heat-resistant 36 kDa
protein, corresponding to crab tropomyosin was identified as the major allergen
of both extracts. In addition, a 41 kDa heat-sensitive protein believed to be
arginine kinase was shown to be a major allergen of the raw extract. Other minor
allergens were also observed at various molecular weights.
Arginine Kinase
4.Arginine supplementation in patients diagnosed with drug-sensitive pulmonary tuberculosis.
Paula Victoria Catherine Y. CHENG ; Paolo Nikolai H. SO ; Rogelio N. VELASCO ; Norman L. MAGHUYOP
Acta Medica Philippina 2018;52(1):69-80
OBJECTIVE: To determine the effects of arginine in the rates of sputum conversion in patients with drug-sensitive pulmonary tuberculosis.
METHODS: Studies from PubMed, Medline, EMBASE, and Cochrane were reviewed and appropriate studies were included. Randomized controlled trials comparing arginine with placebo in adult patients with drug-sensitive pulmonary tuberculosis were included. The risk of bias was assessed using the Cochrane Risk of Bias tool. A meta-analysis of the rate of sputum conversion at 8 weeks, was conducted. Post hoc analyses of sputum conversion at 4 weeks and cough reduction at 4 and 8 weeks were done.
RESULTS: Three articles included in this study had a pooled population of 452 participants. This meta-analysis showed no significant difference in the sputum conversion at 4 and 8 weeks, with a relative risk of 0.96 (95% CI 0.77-1.20) and 1.07 (95% CI 0.96-1.19), respectively. However, the cough was significantly reduced at 4 and 8 weeks, with subtotal relative risks of 0.91 (95% CI 0.82-1.00) and 0.43 (95% CI 0.22-0.81), and total relative risk for cough reduction of 0.83 (95% CI 0.73-0.93).
CONCLUSION: While arginine may not significantly reduce sputum conversion rates, it may be used as an adjunct to decrease cough in patients with tuberculosis.
Human ; Arginine ; Tuberculosis, Pulmonary
5.Altered secretion of arginine vasopressin in children with CNS diseases.
Kun Whe KIM ; Eun Sook KIM ; Cheol Woo KO ; Ja Hoon KOO ; Doo Hong AHN ; Won Jung LEE
Journal of the Korean Pediatric Society 1991;34(3):323-333
No abstract available.
Arginine Vasopressin*
;
Arginine*
;
Central Nervous System Diseases*
;
Child*
;
Humans
6.Altered secretion of arginine vasopressin in children with CNS diseases.
Kun Whe KIM ; Eun Sook KIM ; Cheol Woo KO ; Ja Hoon KOO ; Doo Hong AHN ; Won Jung LEE
Journal of the Korean Pediatric Society 1991;34(3):323-333
No abstract available.
Arginine Vasopressin*
;
Arginine*
;
Central Nervous System Diseases*
;
Child*
;
Humans
7.Development of Simple Identification Method of Enterococci.
Young UH ; In Ho JANG ; Kap Jun YOON
Korean Journal of Clinical Pathology 1999;19(1):57-61
BACKGROUND: The selection of identification (ID) system of enterococci depends mainly on the accuracy of ID system, cost of operation and convenience of testing. Commercial ID kits are easy to use but too expensive. The aim of the study was to develop a simple system for the identification of species of enterococci which are frequently isolated from clinical specimens. METHODS: Eight conventional biochemical tests selected for the simple ID method were hemolysis pattern, NaCl-esculin hydrolysis, tellurite tolerance, arginine dihydrolase, acid from arabinose, raffinose and methyl-alpha-D-glucopyranoside, and pigment production. Ninety one consecutive strains of enterococci from clinical specimens isolated during the period of April 1988 were tested by the simple ID method, and API rapid ID 32 STREP. RESULTS: Simple ID method with 15 ID codes was established to identify 13 species of enterococci. Among the 91 isolates tested, 88 (96.7%) were identified to the species level of enterococci by simple ID method. CONCLUSIONS: The simplified conventional ID method is simple, reliable and economical. Further modification may improve the accuracy of the enterococcal species identification system.
Arabinose
;
Arginine
;
Hemolysis
;
Hydrolysis
;
Raffinose
9.The Role of Urine Osmolality as a Predictor of the Effectiveness of Desmopressin Treatment in Enuretic Children.
Korean Journal of Urology 2000;41(9):1112-1116
No abstract available.
Child*
;
Deamino Arginine Vasopressin*
;
Humans
;
Osmolar Concentration*
10.Identification of a New Selective Chemical Inhibitor of Mutant Isocitrate Dehydrogenase-1.
Hyo Joon KIM ; Bu Young CHOI ; Young Sam KEUM
Journal of Cancer Prevention 2015;20(1):78-83
BACKGROUND: Recent genome-wide sequencing studies have identified unexpected genetic alterations in cancer. In particular, missense mutations in isocitrate dehydrogenase-1 (IDH1) at arginine 132, mostly substituted into histidine (IDH1-R132H) were observed to frequently occur in glioma patients. METHODS: We have purified recombinant IDH1 and IDH1-R132H proteins and monitored their catalytic activities. In parallel experiments, we have attempted to find new selective IDH1-R132H chemical inhibitor(s) from a fragment-based chemical library. RESULTS: We have found that IDH1, but not IDH1-R132H, can catalyze the conversion of isocitrate into alpha-ketoglutarate (alpha-KG). In addition, we have observed that IDH1-R132H was more efficient than IDH1 in converting alpha-KG into (R)-2-hydroxyglutarate (R-2HG). Moreover, we have identified a new hit molecule, e.g., 2-(3-trifluoromethylphenyl)isothioazol-3(2H)-one as a new selective IDH1-R132H inhibitor. CONCLUSIONS: We have observed an underlying biochemical mechanism explaining how a heterozygous IDH1 mutation contributes to the generation of R-2HG and increases cellular histone H3 trimethylation levels. We have also identified a novel selective IDH1-R132H chemical hit molecule, e.g., 2-(3-trifluoromethylphenyl)isothioazol-3(2H)-one, which could be used for a future lead development against IDH1-R132H.
Arginine
;
Glioma
;
Histidine
;
Histones
;
Humans
;
Mutation, Missense