In order to control the quality of Vigna radiata, the quality control method and standard were established in this study. The tests of water content, ash and ethanol-soluble extractives of V. radiata were carried out according to the methods recoded in appendix of Chinese Pharmacopeia (2010 edition, volume 1). The TLC method was established by using vitexin and isovitexin as references, and a mixture of acetate-method-water (10: 1.7 : 1.3) as the developing solvent system on GF254 thin layer plate. The contents of vitexin and isovitexin were determined by HPLC on a Prevail C18 (4.6 mm x 250 mm, 5 microm) column, using acetonitrile: water (23 : 77) as mobile phase at a flow rate of 1.0 mL x min(-1). The column temperature is 30 degrees C and the detection wavelength is 337 nm. As a result, vitexin, isovitexin and the other constituents were well separated on TLC detected under the UV light (254 nm). The methodology validation for the assay of vitexin and isovitexin presented that they were in good linear correlation in the ranges of 6.12-98 mg x L(-1) and 6.85-109.6 mg x L(-1), with the regression equations of Y = 46.213X - 7.100 (r = 1.000) and Y = 54.515X + 6.829 (r = 1.000), and the average recoveries were 98.2% (RSD 1.9%) and 97.2% (RSD 0.79%), respectively. The content ranges of vitexin and isovitexin from 25 different batches of V. radiata were 1.076-2.062 mg x g(1) and 1.127-2.303 mg x g(-1), respectively. suggesting that the qualities of V. radiata are relatively stable. The ethanol-soluble extractives, water content and total ash of 25 samples varied in the ranges of 13.27% - 18.46%, 9.59% - 12.43% and 2.63% - 3.53%, respectively. All of the above data proved that the established quality of control method V. radiata is specific and accurate, which can be used for the quality control of this drug.
Apigenin ; chemistry ; Fabaceae ; chemistry ; Quality Control

OBJECTIVETo study the chemical constituents of Gentiana veitchiorum.

METHODThe chemical constituents were isolated by chromatography and identified by spectral data.

RESULTFive glycosides, loganic acid (1), gentiopicroside (2), isoorientin 3'-methyl ether (3), isovitexin (4), isoorientin (5) were isolated and identified.

CONCLUSIONCompounds 1-5 were isolated from this plant for the first time.

Apigenin ; chemistry ; Chromatography, High Pressure Liquid ; Gentiana ; chemistry ; Glucosides ; chemistry ; Glycosides ; chemistry ; Iridoid Glucosides ; Iridoids ; chemistry

Scutellarin is the major effective ingredient of breviscapine, which is widely used in the treatment of cardiovascular diseases and has a remarkable achievement. In recent years, more and more reseaches had focused on the pharmacokinetics of scutellarin and scutellarein. The article summarized the studies on pharmacokinetics of scutellarin and scutellarein have been performed by domestic and oversea scholars.
Animals ; Apigenin ; pharmacokinetics ; Flavonoids ; chemistry ; Glucuronates ; pharmacokinetics ; Humans

OBJECTIVETo ascertain the optimal harvest time of Erigeron breviscapus.

METHODThe dry matter weight accumulation of different organs in growth process and contents of scutellarin and coffeic acid ester in whole plant of E. breviscapus were determined.

RESULTThe number of leaves per plant, the dried weight of single leaf and dry matter weight of whole plant and different organs reached the highest after seedling 130-140 d. The content of scutellarin gradually decreased with growth period, and sharply decreased after seedling 140 d. The content of coffeic acid ester varied irregularly with growth period.

CONCLUSIONThe optimal harvest time of E. breviscapus is in early bloom period after seedling 130 d.

Apigenin ; analysis ; Biomass ; Erigeron ; chemistry ; growth & development ; Gardening ; Glucuronates ; analysis

OBJECTIVETo investigate the chemical constituents of whole herbs of Seriphidium terrae-albae.

METHODVarious chromatographic techniques were employed for the isolation and purification of the chemical constituents. The structures were elucidated by chemical and spectral analysis.

RESULTSeven flavonoid compounds were isolated and identified as apigenin, eupatilin, chrysoeriol, quercetin-3, 3'-dimethyl ether, quercetagetin-3, 6-dimethyl ether, quercetagetin-3, 6-dimethyl ether-7-O-beta-D-pyranoglucoside and quercetagetin-3, 6-dimethyl ether-4'-O-beta-D-pyranoglucoside, respectively.

CONCLUSIONAll of these flavonoid compounds were isolated from the plant for the first time.

Apigenin ; chemistry ; isolation & purification ; Asteraceae ; chemistry ; Flavones ; Flavonoids ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry

AIMTo study the chemical constituents of Lagopsis supina.

METHODSCompounds were isolated by column chromatography of silica gel and Sephadex LH-20, and the structures were determined by spectral analysis.

RESULTSTwo compounds were isolated and elucidated as apigenin-7-O-(6"-(E)-p-coumaroyl)-beta-D-galactopyranoside (I) and apigenin-7-O-(3",6"-di-(E)-p-coumaroyl)-beta-D-galactopyranoside (II).

CONCLUSIONI and II are new compounds.

Apigenin ; Flavonoids ; chemistry ; isolation & purification ; Galactosides ; chemistry ; isolation & purification ; Lamiaceae ; chemistry ; Molecular Structure ; Plants, Medicinal ; chemistry

OBJECTIVETo study the chemical constituents of Exochorda racemosa.

METHODCompounds were isolated and purified by silica gel, Sephadex LH-20, MCI gel and RP-18 column chromatography, and their structures were determined by spectroscopic analysis.

RESULTTwenty compounds were isolated and identified as N-p-coumaroyl-N'-caffeoylputrescine (1), sutherlandin trans-p-coumarate (2), apigenin 7-O-methylglucuronide (3), astragalin (4), nicotiflorin (5), kaempferol 3-neohesperidoside (6), rutin (7), apigenin (8), luteolin (9), linalool-1-oic acid (10), betulalbuside A (11), ursolic acid (12) , corosolic acid (13), gynuramide II (14), beta-sitosterol (15), daucosterol (16), uridine (17), adenosine (18), syringin (19), and trans4-hydroxycinnamic acid (20), respectively.

CONCLUSIONAll compounds were obtained from this plant for the first time, moreover, 1 was reported as a new natural product, and 2 is a naturally rare cyanogenic glycoside.

Apigenin ; chemistry ; Flavonoids ; chemistry ; Glucosides ; chemistry ; Glucuronides ; chemistry ; Kaempferols ; chemistry ; Luteolin ; chemistry ; Magnetic Resonance Spectroscopy ; Phenols ; chemistry ; Phenylpropionates ; chemistry ; Rosaceae ; chemistry ; Sitosterols ; chemistry ; Triterpenes ; chemistry

OBJECTIVETo investigate the chemical constituents from flowers of Sesamum indicum.

METHODColumn chromatography with silica gel, C18 and Sephadex LH -20 as packing materials was used to separate the chemical constituents, and the structures were determined by chemical and spectroscopic methods.

RESULTSix flavones were isolated and elucidated as apigenin (1), ladanetin (2), ladanetin-6-O-beta-D-glucoside (3), apigenin-7-O-glucuronic acid (4), pedalitin (5), and pedalitin-6-O-glucoside (6).

CONCLUSIONAll of the compounds were isolated from this plant for the first time.

Apigenin ; chemistry ; isolation & purification ; Flavones ; chemistry ; isolation & purification ; Flowers ; chemistry ; Molecular Structure ; Plants, Medicinal ; chemistry ; Sesamum ; chemistry

OBJECTIVETo isolate and identify compounds from Picria fel-tarrae in order to utilize it better.

METHODConstituents from Picria fel-tarrae were isolated by several column chromatography and their structures were elucidated on the basis of chemical and spectral analysis.

RESULTSix compounds, N-benzoylphenylalanyl-L-phenylalaninol acetate (1), 1-hydroxy-7-hydroxymethyl-9,10-anthraquinone (2), 9, 16-dioxo-10,12,14-octadeca-trienoic acid (3), 5,7,4'-trihydroxy-flavone (4), beta-sitosterol (5), and daucosterol (6) were obtained from the fraction with relatively low polarity of EtOH extract of Picria fel-tarrae.

CONCLUSIONCompounds 1-6 were isolated from picria fel-tarrae Lour for the first time, and the 13C-NMR data of compounds 1-3 are provided firstly in the literature.

Apigenin ; chemistry ; isolation & purification ; Dipeptides ; chemistry ; isolation & purification ; Molecular Structure ; Plants, Medicinal ; chemistry ; Scrophulariaceae ; chemistry ; Sitosterols ; chemistry ; isolation & purification

OBJECTIVETo study the chemical constituents from Eremosparton songoricum.

METHODThe compounds were isolated with silica gel column chromatography and the structures of these compounds were elucidated by means of spectral analysis.

RESULTThe seven compounds were identified as: 5,7,4'-trihydroxyflavone (apigenin) (I), 5-hydroxy-7,4'-dimethoxyflavone (II), 5,7-dihydroxy-3',4'-dimethoxyflavone (III), 5,7-dihydroxy-4'-methoxyflavone (acacetin) (IV), 5,7,4'-trihydroxy-3'-methoxyflavone (chrysoeriol) (V), 5,6,3',4'-tetrahydroxy-7-methoxyflavone (pedalitin) (VI) and 5,4'-dihydroxy-7,3'-dimethoxyflavone-4'-O-D-glucoside (flavogadorinin) (VII).

CONCLUSIONThese constituents were obtained from E. songoricum for the first time.

Apigenin ; chemistry ; isolation & purification ; Fabaceae ; chemistry ; Flavones ; chemistry ; isolation & purification ; Flavonoids ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry