This paper summarized the recent 30 years research progress of the chemical constituents from Notopterygii Rhizoma et Radix. The chemical constituents from Notopterygii Rhizoma et Radix mainly consist of coumarins, polyene-polyacetylenes, sesquiterpenes, phenolic acids, while steroids and flavonoids were less reported. All constituents were confirmed and corrected through SciFinder. We also checked the Chinese name and English name and listed the CAS number of each compound. It can provide some guidelines for the research, development and utilization of Notopterygii Rhizoma et Radix in the future. Whether there is columbianin in the Notopterygii Rhizoma et Radix need to be further researched.
Apiaceae ; chemistry ; Drugs, Chinese Herbal ; analysis ; Rhizome ; chemistry

OBJECTIVETo study the chemical constituents in the dried roots of Saposhnikovia divaricata.

METHODThe chemical constituents were isolated by various column chromatographic methods and structurally elucidated by IR, UV, MS and NMR evidences.

RESULTEighteen compounds were obtained and identified as 3'-O-angeloylhamaudol (1), isobergapten (2), imperatorin (3), pentacosane acid (4), anomalin (5), decursin (6), 5-methoxy-7-(3,3-dimethylallyl- oxy)coumarin (7), decursinol angelate (8), xanthotoxin (9), bergapten (10), tectochrysin (11), scopoletin (12), hamaudol (13), ledebouriellol (14), cimifugin (15), sec-O-glucosylhamaudol (16), 4'-O-beta-D-glucosyl-5-O-methylvisamminol (17), and prim-O-glucosylcimifugin (18).

CONCLUSIONCompounds 2, 6-8, and 11 were isolated from the roots of S. divaricata for the first time. Compounds 1 and 13-18 were chromones, 2, 3, 5-10 and 12 were coumarins, 4 was fatty acid, and 11 was flavonoid.

OBJECTIVETo compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata.

METHODThe separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).

RESULTAll the obtained correlation coefficient of the six components was over 0.9995 and the average recoveries were not less than 97% (RSD <5%), respectively.

CONCLUSIONThe method is simple, accurate with good reproducibility. Results showed the better preparation procedure is water-decoction and ethanol-precipitation with resin separation and silica gel purification.

Saposhnikoviae divaricata (Turcz.) Schischk (SD) is a traditional Chinese herb commonly used to treat clinical conditions such as rheumatism and allergic rhinitis. This review article evaluates a collection of works on in vitro and biochemical studies of SD. The discourse on the diverse class of chromones and coumarins in SD offers an insight to the pharmacological effects of these bioactive constituents as anti-inflammatory, analgesic, immunoregulatory, antioxidative, and anti-proliferative agents. It is highlighted that there is a structural relationship between the constituents and bioactive activities, which in effect provides a valid reasoning and reaffirm the use of SD in the treatment of the pathologies in Chinese medicine.
Animals ; Apiaceae ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacokinetics ; pharmacology ; Humans

OBJECTIVETo explore the best pretreatment condition of inorganic salting on volatile oil-bearing water body of Notopterygii Rhizoma et Radix before membrane separation.

METHODThe simulative system of volatile oil-bearing water body of Notopterygii Rhizoma et Radix was pre-treated before membrane separation by salting. The best conditions of salting were determined by selecting types and amounts of inorganic salt as investigate factors and comparing membrane flux and oil retention rate.

RESULTThe best pretreatment condition of inorganic salting on volatile oil-bearing water body of Notopterygii Rhizoma et Radix before membrane separation was to add 2% sodium chloride (NaCl) according to the amount of oil-bearing water body. Gas chromatographic fingerprint showed that inorganic salting did not affect the active ingredient of volatile oil.

CONCLUSIONAdding NaCl to volatile oil-bearing water body of Notopterygii Rhizoma et Radix before membrane separation can optimize membrane processes by improving membrane flux.

Common and variation peak rates were calculated as dual-index for drug samples based on the infrared spectrogram of Angelicae Pubescentis Radix samples from different places. The results indicated that the common peak rates of 4 samples from Sichuan province, 2 samples from Gansu province, and 2 samples from Hubei province were higher than 73% , 79. 5% and 76. 3%, respectively. Also, the variation peak rates of 2 samples from Gansu province were lower than 12. 9%. Therefore, there is similar germplasm resources and similar chemical composition in the samples originated from the same place. However, for the samples originated from different places, their germplasm resources and chemical composition maybe have been changed. Fourier transform infrared spectrometry could be used to evaluate the rule of the fingerprint and overview of Angelicae Pubescentis Radix originated from different places by its changes of germplasm resources and chemical composition.
Apiaceae ; chemistry ; Medicine, Chinese Traditional ; Spectroscopy, Fourier Transform Infrared ; methods

Saposhnikovia divaricata (Turcz.) Schischk (S. divaricata, Fangfeng) is a herb in the Apiaceae family, and its root has been used since the Western Han Dynasty (202 B.C.). Chromones and coumarins are the pharmacologically active substances in S. divaricata. Modern phytochemical and pharmacological studies have demonstrated their antipyretic, analgesic, anti-inflammatory, antioxidant, anti-tumor, and anticoagulant activities. Technological and analytical strategy theory advancements have yielded novel results; however, most investigations have been limited to the main active substances-chromones and coumarins. Hence, we reviewed studies related to the chemical composition and pharmacological activity of S. divaricata, analyzed the developing trends and challenges, and proposed that research should focus on components' synergistic effects. We also suggested that, the structure-effect relationship should be prioritized in advanced research.
Drugs, Chinese Herbal/pharmacology* ; Coumarins/pharmacology* ; Apiaceae/chemistry* ; Chromones

Sixteen compounds were isolated from Conioselinum vaginatum by silica column chromatography over silica gel and Sephadex LH-20, as well as recrystallization. On the basis of their physical and chemical properties and spectral data, their structures were identified as ligustilide (1), 1,3-dilinolein (2), coniferaldehyde (3), myristicin (4), stigmasterol (5), beta-sitosterol (6), vanillin (7), pregnenolone (8), bergapten (9), xanthotoxin (10), methyl indole-3-carboxylate (11), ferulic acid (12), (E)-3-methoxy-4,5-methylenedioxy-cinnamic alcohol (13), p-hydroxybenzaldehyde (14), 3-methoxy-4,5-methylenedioxy-acetophenone (15), and alpha-(ethoxymethyl)-4-hydroxy- benzenemethanol (16). Among them, compound 15 was a new natural product, and compounds 2, 3, 10, 11, 14, and 16 were obtained from this genus for the first time.
Apiaceae ; chemistry ; Benzaldehydes ; chemistry ; Dextrans ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Mass Spectrometry ; Molecular Structure ; Sitosterols ; chemistry

The root of Peucedanum harry-smithii var. subglabrum was extracted with methanol, then separated with solvents at different polarity into four fractions: aqueous (H2O), ethyl acetate (AcOEt), chloroform (CHCl3) and petroleum ether (DAB-6). From AcOEt psoralen, bargapten, xanthotoxin, marmesin, umbelliferone, scopoletin, (+/-) peuformosin, Pd-I b, (+/-) selinidin, praeruptorin D were isolated by column chromatography on silica gel, using petroleum ether/ethyl acetate as eluent. The structures of the coumarins were identified by 1H-NMR and 13C-NMR.
Apiaceae ; chemistry ; Coumarins ; chemistry ; isolation & purification ; Magnetic Resonance Spectroscopy ; methods ; Plant Roots ; chemistry ; Umbelliferones ; chemistry

OBJECTIVETo study the chemical constituents in the roots and rhizomes of Notopterygium incisum.

METHODThe chemical constituents were isolated by various column chromatographic methods and structurally elucidated by NMR and MS evidences.

RESULTTwenty-four compounds were obtained and identified as isocalamenediol (1), pregnenolone (2), beta-sitosterol (3), bergapten (4), bergaptol (5), cnidilin (6), isoimperatorin (7), bergamottin (8), demethylfuropinnarin (9), nodakenetin (10), 7'-O-methylnotoptol (11), notopterol (12), notoptol (13), pabulenol (14), phenethylferulate (15), p-hydroxyphenethyl anisate (16), trans-ferulic acid (17), nodakenin (18), sucrose (19), 2-methoxy-4-(3-methoxy-1-propenyl)-phenol (20), 3, 4, 5 trimethoxy-trans-cinnamic acid (21), p-methoxycinnamic acid (22), 4-acetoxy-3-methoxy-trans-cinnamic acid (23), and p-hydroxy-trans-cinnamic acid (24).

CONCLUSIONCompounds 1, 14, 20-24 were isolated from the roots and rhizomes of N. incisum for the first time. Compounds 1 and 14 are sesquiterpenoid and coumarin, respectively, and 20-24 were phenylpropanoid compounds.