1.The effect of Astragalus membranaceus (Fisch.) Bge. on intestinal function and gut microbiota homeostasis in aging mice
Jia-ting YIN ; Shu-hui YANG ; Cheng-xi LI ; Yun LIU ; Yu-meng WANG ; Jin-ao DUAN ; Jian-ming GUO
Acta Pharmaceutica Sinica 2022;57(12):3535-3545
Aging can cause degenerative changes in the function of multiple tissues and organs in the body. Gastrointestinal diseases and intestinal dysfunction are very common in the elderly people. The purpose of this study is to explore the effect of the total extract of
2.Analysis of the research status and intervention strategies for the treatment of hepatic encephalopathy based on gut microbiota regulation
Jia-ting YIN ; Yin PENG ; Wen-hao XU ; Meng-fei MAO ; Jin-ao DUAN ; Jian-ming GUO
Acta Pharmaceutica Sinica 2021;56(4):895-905
Hepatic encephalopathy is a common metabolic neuropsychiatric syndrome in the development of end-stage liver disease. Since the concept of intestinal-liver-brain axis was proposed, the relationship between the pathogenesis of hepatic encephalopathy and the gut microbiota has been a hot research topic. In recent years, studies have confirmed that gut microbiota is involved in and affects various pathological processes of hepatic encephalopathy. This article combines the latest research progress at home and abroad to elaborate on the research status of regulating gut microbiota and thus interfering with the pathological process of hepatic encephalopathy, hoping to provide new ideas and methods for the intervention of hepatic encephalopathy based on the regulation of gut microbiota.
3.Piceatannol alleviates host inflammation in chronic kidney disease model mice through regulating gut microbiota
Cheng-xi LI ; Ying-yi WANG ; Yu-meng WANG ; Jia-ting YIN ; Shu-hui YANG ; Yun LIU ; Jin-ao DUAN ; Jian-ming GUO
Acta Pharmaceutica Sinica 2022;57(2):364-374
The purpose of this research is to study the effect of small molecule compound piceatannol (PIC) on host inflammation in adenine induced chronic kidney disease (CKD) mice, and then to explore its mechanism based on the regulation of gut microbiota. All procedures were approved by the Institutional Animal Care and Use Committee of the Nanjing University of Chinese Medicine. The level of interleukin-6 (IL-6) and tumor necrosis factor-
4.Gene expression profiles in early stage of BALB/c 3T3 cells' transformation promoted with 12-O-tetradecanoylphorbol-13-acetate.
Lin AO ; Jia CAO ; Ming-hui HUANG ; Ya-zhou WU ; Jin-yi LIU ; Yan-qi ZHANG ; Zhi-xiong ZENG ; Meng-su YANG
Chinese Journal of Preventive Medicine 2005;39(2):99-102
OBJECTIVETo elucidate the potential molecular mechanism responsible for the early time of tumor promotion, gene expression profile was studied in the transformed BALB/c 3T3 cells induced by 12-O-tetradecanoylphorbol-13-acetate (TPA).
METHODSThe two-stage cell transformation model was established by using the initiator of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and promoter of TPA. Cell proliferation was measured by trypan blue staining and cell cycle analysis was carried out by flow cytometry assay. A cDNA microarray representing 1 152 genes was used to investigate the gene expression profiles of BALB/c 3T3 cells exposed to TPA at 4 h and 24 h respectively.
RESULTSTPA could effectively inhibit cell proliferation and induce the G1 and S cell cycle arrested in the early time. Moreover 19 genes were found differentially expressed at least twofold in the TPA treated cells as compared with the control cells, 9 of them were upregulated and 10 downregulated. Most of the differentially expressed genes were involved in cell proliferation, differentiation or apoptosis, and related to ras or p53 signal transduction pathway.
CONCLUSIONTPA could influence the transcriptional expression of some genes related to cell cycle modulation and ultimately result in the cell growth arrest.
Animals ; Apoptosis ; drug effects ; genetics ; BALB 3T3 Cells ; Cell Cycle ; drug effects ; genetics ; Cell Differentiation ; drug effects ; genetics ; Cell Proliferation ; drug effects ; Cell Transformation, Neoplastic ; drug effects ; genetics ; Flow Cytometry ; Gene Expression ; drug effects ; Gene Expression Profiling ; Methylnitronitrosoguanidine ; pharmacology ; Mice ; Oligonucleotide Array Sequence Analysis ; methods ; Tetradecanoylphorbol Acetate ; analogs & derivatives ; pharmacology
5.Advance in Model Establishment for Denervated Skeletal Muscle Atrophy (review)
Dan Dan- ZHAO ; Lin Cheng- TANG ; Qin Si- HUANG ; Ao LUO ; Ning An- ZHANG ; Yu Hui- AN ; Jia Meng- WU ; Cheng-Fang TAN
Chinese Journal of Rehabilitation Theory and Practice 2018;24(1):80-83
The skeletal muscle atrophy could be induced by the injury of nerve. According to the source of denervated skeletal mus-cle atrophy, it could be divided into exogenous muscle atrophy and endogenous muscle atrophy. In recent years, the ex-ogenous muscle atrophy models are mainly established by operating, physically injuring or chemically injuring, while the endogenous muscle atrophy models are mainly established by the transgenic animals of amyotrophic lateral sclero-sis. The selection and optimazation of animal models are crucial for the basic studies of denervated skeletal muscle atro-phy.
6.Effects of Electroacupuncture on Autophagy-related Gene Expression in Skeletal Muscle in Denervated Rats
Meng-Jia WU ; Cheng-Lin TANG ; Dan-Dan ZHAO ; Ao LUO ; Hui-Yu AN ; Cheng-Fang TAN ; Li QIU
Chinese Journal of Rehabilitation Theory and Practice 2018;24(3):260-265
Objective To explore the effects and mechanism of electroacupuncture(EA)on denervation-induced atrophy. Methods A total of 21 male Sprague-Dawley rats were divided into sham group(n=7),model group(n=7)and EA group (n=7).The latter two groups were cut off their right sciatic nerve.Since one day after modeling,EA group accept-ed electroacupuncture at right Zusanli(ST36)and Huantiao(GB30)for eight weeks.Then,the gastrocnemius of all the rats were obtained,and measured the wet mass ratio.Cross-sectional area(CSA)and fiber diameter were measured after HE staining. The expression of autophagy-related gene ULK1, Atg13, Beclin1, Atg14, Atg7, Atg12,Atg5 and Atg16L1 were tested with reverse transcription real-time quantitative polymerase chain reaction. Results Compared with the sham group,the wet mass ratio,CSA and fiber diameter of gastrocnemius were lower signifi-cantly in the model group and EA group(P<0.001),and they were more in EA group than in the model group(P<0.05).Compared with the sham group,the mRNA expression of ULK1,Atg13,Beclin1,Atg14,Atg7,Atg12,Atg5 and Atg16L1 was more significantly in the model group (P<0.001), and they decreased in EA group compared with those of the model group(P<0.05). Conclusion Electroacupuncture can inhibit the overexpression of autophagy-related gene in denervated rats,which may steady skeletal muscle cells to delay atrophy.
7.Effect of Tuina on Autophagy-related Factor Beclin-1, Vacuolar Protein Sorting and Microtubule-associated Protein Light Chain 3 in Rats with Denervated Skeletal Muscle Atrophy
Hui-yu AN ; Cheng-lin TANG ; Si-qin HUANG ; Dan-dan ZHAO ; Ao LUO ; Meng-jia WU ; Cheng-fang TAN ; Li QIU ; Xiao-feng WAN ; Xiang MA
Chinese Journal of Rehabilitation Theory and Practice 2019;25(2):184-191
Objective:To explore the effect and mechanism of Tuina on denervated skeletal muscle atrophy. Methods:A total of 77 male Sprague-Dawley rats were randomly divided into sham group (
8.Rapid identification and differential markers of Curcumae Radix decoction pieces of different sources based on Heracles Neo ultra-fast gas phase electronic nose.
Ming-Xuan LI ; Yu-Wen QIN ; Yu LI ; Jiu-Ba ZHANG ; De JI ; Ling-Yun QU ; Jing-Wen GONG ; Ao-Meng JIA ; Chun-Qin MAO ; Tu-Lin LU
China Journal of Chinese Materia Medica 2023;48(6):1518-1525
Since Curcumae Radix decoction pieces have multiple sources, it is difficult to distinguish depending on traditional cha-racters, and the mixed use of multi-source Curcumae Radix will affect its clinical efficacy. Heracles Neo ultra-fast gas phase electronic nose was used in this study to quickly identify and analyze the odor components of 40 batches of Curcumae Radix samples from Sichuan, Zhejiang, and Guangxi. Based on the odor fingerprints established for Curcumae Radix decoction pieces of multiple sources, the odor components was identified and analyzed, and the chromatographic peaks were processed and analyzed to establish a rapid identification method. Principal component analysis(PCA), discriminant factor analysis(DFA), and soft independent modeling cluster analysis(SIMCA) were constructed for verification. At the same time, one-way analysis of variance(ANOVA) combined with variable importance in projection(VIP) was employed to screen out the odor components with P<0.05 and VIP>1, and 13 odor components such as β-caryophyllene and limonene were hypothesized as the odor differential markers of Curcumae Radix decoction pieces of diffe-rent sources. The results showed that Heracles Neo ultra-fast gas phase electronic nose can well analyze the odor characteristics and rapidly and accurately discriminate Curcumae Radix decoction pieces of different sources. It can be applied to the quality control(e.g., online detection) in the production of Curcumae Radix decoction pieces. This study provides a new method and idea for the rapid identification and quality control of Curcumae Radix decoction pieces.
Drugs, Chinese Herbal/analysis*
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Electronic Nose
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China
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Plant Roots/chemistry*
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Limonene/analysis*
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Chromatography, High Pressure Liquid
9.The expression mechanism of programmed cell death 1 ligand 1 and its role in immunomodulatory ability of mesenchymal stem cells.
Zhuo CHEN ; Meng-Wei YAO ; Xiang AO ; Qing-Jia GONG ; Yi YANG ; Jin-Xia LIU ; Qi-Zhou LIAN ; Xiang XU ; Ling-Jing ZUO
Chinese Journal of Traumatology 2024;27(1):1-10
Programmed cell death 1 ligand 1 (PD-L1) is an important immunosuppressive molecule, which inhibits the function of T cells and other immune cells by binding to the receptor programmed cell death-1. The PD-L1 expression disorder plays an important role in the occurrence, development, and treatment of sepsis or other inflammatory diseases, and has become an important target for the treatment of these diseases. Mesenchymal stem cells (MSCs) are a kind of pluripotent stem cells with multiple differentiation potential. In recent years, MSCs have been found to have a strong immunosuppressive ability and are used to treat various inflammatory insults caused by hyperimmune diseases. Moreover, PD-L1 is deeply involved in the immunosuppressive events of MSCs and plays an important role in the treatment of various diseases. In this review, we will summarize the main regulatory mechanism of PD-L1 expression, and discuss various biological functions of PD-L1 in the immune regulation of MSCs.
Humans
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B7-H1 Antigen/metabolism*
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Mesenchymal Stem Cells/immunology*
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T-Lymphocytes/metabolism*
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Immunomodulation
10.Textual and information research of classical prescription Mijiao Pills.
Xue-Er MENG ; Yue ZHU ; Yu-Hua DING ; Jia-de BAI ; Hua-Jun WEN ; Hai-Rong WU ; Bin YANG ; Zhen-Yu ZHONG ; Jin-Ao DUAN ; Ming ZHAO
China Journal of Chinese Materia Medica 2021;46(14):3478-3486
Elaphurus davidianus( Milu),a rare animal unique to China,has been used as medicine for more than a thousand years,but the extinction of Milu in modern times resulted in the unavailability of related medical products. Today,the reintroduction of Milu population makes it possible to restore its medicinal usage. The resource reserves of Cervi Cornu,the natural shedding product from Milu,are increasing with the expansion of the population,allowing it to be fully utilized in the medical field. Mijiao Pills,first recorded in Important Prescriptions Worth a Thousand Gold for Emergency( Bei Ji Qian Jin Yao Fang) by Sun Simiao in the Tang Dynasty,is the first Chinese medicinal prescription with Cervi Cornu as the sovereign medicinal,which is effective in tonifying. Its composition,preparation,efficacy and indications,and administration are described in detail in the Important Prescriptions Worth a Thousand Gold for Emergency,which however,have changed significantly over the thousands of years,seriously affecting the clinical application of this classical prescription and related product development. Therefore,the key information of this prescription should be systematically collated and summarized. According to the principles of textual research on key information in ancient classical prescriptions promulgated by relevant authorities,this paper reviewed ancient Chinese medical books of the past dynasties,modern literature,as well as the Pharmacopoeia of the People's Republic of China( 2020 Version) to figure out such key information as the source,historical evolution,original plants and animals and their processing,dosage,preparation,and usage of Mijiao Pills. This paper aimed to provide a basis for the clinical application of Mijiao Pills and subsequent product development,thus facilitating the development and utilization of this precious medicinal animal resource.
Books
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China
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Cornus
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Humans
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Medicine, Chinese Traditional
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Prescriptions