Introduction: Mongolian Traditional Medicine development has been rapid in recent years, so we need to pay attention on one part of treatment which is plant parts. It is a drug safety issue to standardize the traditional medicine. It is necessary to investigate biological active substances in plant, animal and mineral origin drug sources for the further evidence based usage of traditional recipe. Purpose: The determination of biological active substances in traditional “Suman mod-4” recipe. Methodology: To determine “Suman mod-4” (consist of Caesalpinia sappan L., Kaempferia galangal L., Daemonorops dracon Bl, Sal-Ammoniac) traditional medicine’s active constituents, by Chinese and Mongolian National Pharmacopeia (2012) were used thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Result: We developed optimal methodology for the identifying of biological active substances in “Suman mod-4” compound widely used in Mongolian traditional medical practice. And were determined biological active substance measurements in four samples of traditional recipe “Suman mod-4’ from different drug factories in Inner Mongolia. Conclusion: We determined amount of biological active substances in traditional “Suman mod-4” recipe: Bracial Caesalpinia sappan L tissue consist of 2.9847- 3.6716 mg/g, and B Caesalpinia sappan L tissue consist of 2.9214-3.4951 mg/g active ingredients respectively.