Objective To determine the content of anthraquinone in Rumex Patientia L.Methods Through colorimetric technique,used 1,8-Dihydroxyanthraquinone for comparison,0.8% magnesium acetatemethanol solution for coloration,and determined the content of anthraquinone in Rumex Patientia L extract under 510 nm wavelength with colorimetry.Results The content of anthraquinone in Rumex Patientia L extract was 31.07 mg/g,the most of which was bound anthraquinone.Conclusion Colorimetry can be used for the determination of the content of anthraquinone in Rumex Patientia L extract.