Objective To investigate the effect of chronic infection of Toxoplasma gondii on the spatial learning and memory capability in mice.Methods Toxoplasma tachyzoites(RH strain)were reanimated at 37 ℃ after 15 days' storage at-20 ℃,and injected intraperitoneally to mice of the experimental group each with 7.7?105.Normal saline was given to the control group,0.5 ml per mouse.Two months later,all mice were tested in the Morris Water Maze.Smears of the mice brain homogenate and pathological sections were examined.Results ① The density of cysts in the brain homogenate was 15/HP,and there was no evident pathological change in the hippocampus and adjacent areas of mice in the brain in the experimental mice.② Latency to platform,cumulative distance to the platform,total distance traveled in both experimental and control groups decreased significantly with the increase of training days(P

Objective The Female Sexual Function Index ( FSFI) was used to investigate the sexual life quality of patients who got laparoscopic peritoneal vaginoplasty of LuohuⅡ, so as to take measures to improve patients postoperative quality of life. Methods A total of 106 cases undergoing laparoscopic peritoneal vaginoplasty of LuohuⅡ were selected from January 2012 to March 2013. All patients got the questionnaire survey by FSFI and the FSDS-R questionnaire to evaluate the sexual function and psychological states. According to the existing problems, the intervention measures and health guidance were given to patients. And one year after surgery, their sexual function was evaluated again. Results The length of vagina was (12. 29 ± 1. 67) cm, which was significantly longer than before (t =2. 825, P <0. 05). The sexual desire, sexual arousal, sexual orgasm, sexual satisfaction, sexual pain and total score of FSFI were (4. 0 ± 0. 4), (4. 2 ± 0. 4), (4. 5 ± 0. 5), (4. 1 ± 0. 7), (5. 0 ± 0. 5), (4. 6 ± 0. 5) and (29. 7 ± 1. 2), which were significantly better than those before (t=7. 64, 3. 77, 6. 27, 8. 14, 8. 78, 7. 92, 36. 13, respectively;P <0. 01). Conclusions The sexual satisfaction of patients is increased after intervention. The patients with laparoscopic peritoneal vaginoplasty generally have sex problems and lack of the confidence, and effective intervention can improve the sexual life quality and satisfaction, as well as self-confidence of patients.

Objective To analyze the effect of clinical application of ultrasound in microsurgical treatment of intramedullary tumors in the superior cervical spinal cord.Methods Retrospective study the clinical data of 15 patients with intramedullary tumors in the superior cervical spinal cord,which were underwent a laminectomy for microsurgical tumor resection during January,2014 and January,2018.Intraoperative ultrasound and neuromonitoring was accompanied by the whole surgical procedure for each case.The follow-up data was collected by outpatient department visits and telephone interviews.Results All the described patients were performed with microscopic tumor resection by using intraoperative neurophysiological monitoring and ultrasound.The pathological diagnosis was ependymocytoma (n=8) and astrocytoma (n=7).Gross total resections comprised 86.7％ of cases (n=13),and subtotal resections 13.3％ (n=2).The neurological outcome was as follows:Mc-Cormick scale grade Ⅰ,10 patients;grade Ⅱ,3 patients;grade Ⅲ,1 patient;and grade Ⅳ 1 patient;Follow-up was applied for (19.2±7.6) months in 13 cases and 12.0 months in 2 cases.Compared to the preoperative period,66.6％ of patients recovered postoperatively,20.0％ improved,6.7％ remained without deficit and deterioration persisted in 6.7％.Conclusion The microscopic resection of tumors is the effective way to cure this disease.By using intraoperative neurophysiological monitoring and ultrasound,the complete tumor resection and the minimal spinal cord injury were certainly achieved.

Objective To analyze the application value and clinical experience of intraoperative ultrasound in microsurgical resection of supratentorial cavernous malformations.Methods From March 2018 to March 2019,31 patients with supratentorial cavernous malformations were operated with assistance of intraoperative ultrasound in our hospital.Intraoperative ultrasound could clearly show the locations and sizes of the lesions,and relations of lesions with surrounding structures to choose appropriate surgical routes.The degrees of resection were judged and complications were recorded.The patients were followed up for 3-6 months,and modified Rankin scale (mRS) was used to evaluate the prognoses.Results In these 31 patients,35 cavernous hemangiomas were diagnosed by MR imaging before surgery;33 lesions were removed surgically,and the other two lesions located in the contralateral side of the operative field were not removed.The accuracy of intraoperative ultrasound guiding the lesions was up to 100％.There were no postoperative infections or deaths.During follow-up,mRS score of 28 patients was 0;the other 3 patients with postoperative neurological impairment recovered partially during follow-up.Conclusion With the help of real-time monitoring of intraoperative ultrasound and location technique of small pieces of hemostatic yam,patients with supratentorial cavernous malformations could acquire satisfactory prognosis through meticulous microsurgery.