Purpose To study the value of the ultrasonic imaging in the diagnosis and differential diagnosis of benign and malignant breast tumor. Methods Color doppler ultrasound examination were conducted in 262 cases of patients with breast tumor. The mor-phology of the tumor, edge, envelope, internal echo, microcalcification, length and breadth ratio, axillary lymph node and blood-flow signals of color doppler in breast tumors were observed and these results were compared with the pathological observation postoperation. Results There were 314 tumors in all cases, the coincidence rate in the diagnosis of benign breast diseases with ultrasonic imaging and pathological diagnosis was 97. 3%, the misdiagnosis rate was 2. 7%. The coincidence rate in the diagnosis of breast malignant tumors with ultrasound imaging and pathological diagnosis was 92. 9%, the misdiagnosis rate was 7. 1%. There were statistically signif-icant differences between benign tumors and malignant tumors in the tumor shape, edge, echo, microcalcification, length and breadth ratio, axillary lymph node imaging characteristics (P<0. 01). Conclusion Ultrasound imaging can be more accurate diagnosis and i-dentification of benign and malignant breast tumors.

【Objective】 To explore the blood donation status of voluntary donors aged 55 to 60, and to provide references for the publicity and recruitment of elderly blood donors in China. 【Methods】 Based on statistics of voluntary blood donors in Anyang city from 2012 to 2020, 18~54 years old blood donors were taken as the control group, the annual growth rate, gender, deferral rate and the incidence of adverse actions to blood donation of blood donors aged 55~60 were analyzed. 【Results】 Blood donors in Anyang has been increasing year by year, and the annual increase of blood donors aged 55 to 60 was significant(34.64%). Male blood donors were always more than women, however the ratio of men to women has decreased in recent years(269.3 in the 55~60 year old group significantly higher than that in the 18~54 age group). In the same group, the deferral rate of male blood donors was higher than that of female. The deferral rate of blood donors in the 55~60 age group(1.44%) was significantly lower than that in the 18~54 age group(2.62%), with 55~60 aged female blood donors as the lowest(1.33%). Significant difference was observed in the annual growth rate, gender and unqualified rate of blood donors between the two groups, but no difference was noticed in the incidence of adverse reactions. 【Conclusion】 Timely adjusting publicity and recruitment strategies to retain 55~60 year old blood donors as regular blood donors can relieve the pressure of clinical blood supply, which is a helpful complement to develop regular blood donors from low-risk young blood donors.

OBJECTIVETo explore the pattern of lymph node metastasis (LNM) in advanced Siewert type II adenocarcinoma of the gastroesophageal junction (AGEJ) in order to properly guide lymphadenectomy.

METHODSFrom January 2009 to Jun 2011, a total of 86 patients with advanced Siewert type II AGEJ underwent radical esophagogastrectomy by thoracic-abdominal incision and two-field lymphadenectomy. The clinical characteristics, pathologic features, LNM, and influence factor of thoracic metastasis were retrospectively analyzed.

RESULTSLNM was observed in 65 of the 86 cases (75.6%). Simple abdominal lymph node metastasis was observed in 49 of the 86 cases (57.0%), simple thoracic lymph node metastasis was 2.3%, and thoracic-abdominal metastasis was 16.3%, with a significant difference between the abdominal and thoracic metastatic patterns. LNM frequency was found in the lymph node groups No. 3, No. 1, No. 7, No. 110, No. 2 and No. 9 (from the highest to the lowest). The incidences of those lymph node metastases were 46.5%, 41.9%, 17.4%, 14.0%, 10.5%, and 5.8%, respectively. Vascular tumor embolus was the only independent factor for thoracic lymph node metastasis.

CONCLUSIONSAbdominal lymph node metastases of advanced Siewert type II AGEJ mainly occur around the proximal stomach and the coeliac trunk. The metastatic rate of distal stomach and splenic perihilar lymph nodes is low, but metastatic rate of the group No.110 lymph nodes is high. The thoracic lymph node metastasis is only correlated with vascular tumor embolus.

Abdominal Cavity ; Adenocarcinoma ; pathology ; surgery ; Adult ; Aged ; Aged, 80 and over ; Esophageal Neoplasms ; pathology ; surgery ; Esophagogastric Junction ; Female ; Gastrectomy ; Humans ; Lymph Node Excision ; Lymph Nodes ; pathology ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Staging ; Neoplastic Cells, Circulating ; Stomach Neoplasms ; pathology ; surgery ; Thorax

Objective: To explore the effectiveness of art therapy based on painting in ADHD intervene for classroom interfering behaviors. Methods: A total of 60 children with ADHD were randomly selected from three primary schools in Wenfeng District, Anyang City, Henan Province. All the children were randomly divided into two groups. The control group received no intervention. The observation group was intervened with painting art for 8 weeks. The achievement rate of classroom goal behavior and the scores of Conners children s behavior questionnaire were compared before and after the experiment. Results: The achievement rate of all classroom target behaviors (no noise in class, no repetition of teachers speech in class, obeying instructions, no leaving the seat without permission in class, participating in classroom activities) in the observation group was significantly higher after intervention ( χ 2=23.65, 30.54,18.54, 16.98, 20.34, P <0.05), and significantly higher than that in the control group ( χ 2=16.22, 36.02, 24.43 , 38.55, 16.95, P <0.05). The results of Conners children s behavior questionnaire showed that the scores of learning problems, impulse/hyperactivity and hyperactivity index in the observation group were significantly lower after intervention ( Z =7.63, 8.42 , 9.56, P <0.05), and significantly lower than those in the control group ( Z =10.81, 11.25, 10.16, P <0.05). Teacher rated Conners children s behavior questionnaire showed that the scores of behavior problems, hyperactivity, inattention passivity and hyperactivity index in the observation group were significantly lower ( Z =6.32, 7.56, 10.03, 9.18, P ＜0.05), and significantly lower than those in the control group ( Z =11.66, 19.22, 12.24, 9.01, P <0.05). Conclusion Art therapy based on painting in ADHD intervention can effectively reduce classroom interfering behaviors, promote concentration and improve ADHD symptoms.

AIM: To investigate the changes of visual function and multifocal visual evoked potential in children with strabismus after intermittent exotropia operation.

METHODS: Totally 91 children with intermittent exotropia from January 2014 to December 2017 were enrolled in the study. All children were treated with surgery. The changes of visual function before and after operation were observed, and the level of multifocal visual evoked potential before and after operation were detected.

RESULTS: The total effective rate was 88% at one week after operation, 86% at 6mo after operation, the mean preoperative strabismus was-38.12△, and the mean postoperative strabismus was-5.8△. At 6mo after operation, the number of children with simultaneous vision, fusion and distant stereopsis decreased significantly as compared with that before operation(P<0.01). The orthopedic rate was 68% at 6mo after operation, and the majority of the patients with all or only one or two grade visual function before the operation were positive, which was significantly higher than that of the patients who lost it after operation(P<0.05). From before operation to 6mo after operation, the amplitude response density of the characteristic peak and the latency of the nasal and temporal retinas in the fourth and sixth rings of the children were significantly increased, while the latent period of the inferior retina was significantly decreased(P<0.05).

CONCLUSION: Intermittent exotropia operation can effectively improve the visual function of children and promote the recovery of eye position in children, and the level of multifocal visual evoked potential in children after operation is also improved to some extent. The clinical effect is outstanding, and it is worth popularizing and applying.

Aim To investigate the effect of nifedipine on the formation of autophagosomes in hepatoma cell line Huh-7 and its mechanism.Methods Different concentrations of nifedipine were used to interfere with the proliferation of Huh-7 cells in vitro.The effect of nifedipine on the proliferation of Huh-7 cells was detected by cell proliferation experiment and colony formation experiment.The expressions of Beclin1 and LC3B-Ⅱ were detected by Western blot.The effect of nifedipine on the formation of autophagosomes in Huh-7 cells was observed by laser scanning confocal microscopy.Results Nifedipine significantly inhibited the proliferation of Huh-7 cells in a time-and concentration-dependent manner.The IC50 of nifedipine on day 2 was 22.7 mg·L-1.Nifedipine at the concentration of 25 mg·L-1 significantly reduced the colony formation rate of Huh-7 cells compared with the control group, and the inhibition rate of colony formation was(95.46±0.45)%.Western blot analysis showed that nifedipine significantly up-regulated the protein expression levels of Beclin1 and LC3B-Ⅱ.The amount of autophagosomes in nifedipine group cells were more than that of control group, which was observed by laser scanning confocal microscopy.Conclusions Nifedipine significantly inhibits the proliferation of Huh-7 cells and promotes the formation of autophagosomes, which may be related to the up-regulation of Beclin1 protein expression by nifedipine.

Objective    To investigate the value of esophageal activity examination under X-ray fluoroscopy and chest CT in evaluating the resectability of upper thoracic esophageal cancer. Methods    A total of 221 upper thoracic esophageal carcinoma patients underwent radical operation between 2009 and 2015 in our hospital were enrolled, including 141 males and 80 females with a median age of 59 (47-79) years. Preoperative routine esophageal activity examination under X-ray fluoroscopy and chest enhanced CT were performed to determine whether the tumor had external invasion. The results of the two methods were compared with that observed during the operation. Results    The number of patients with esophageal activity score 1-6 was 70, 85, 32, 29, 2 and 3, respectively. The area under the receiver operating characteristic (ROC) curve (AUC) of esophageal activity examination was 0.897 (95%CI 0.85-0.93, P<0.001), and the cut off value was >3. According to the ROC curve activity score, 4-6 points were considered as invasion, and 1-3 points were non-invasion. The sensitivity, specificity, accuracy and misdiagnosis rate of esophageal activity examination was 75.0%, 89.3%, 88.2%, 10.7%, respectively, and those of CT scan were 75.0%, 66.8%, 67.4%, 33.2%, respectively. Compared with CT scan, the specificity of esophageal activity examination was higher and the misdiagnosis rate was lower. Compared with the detection during the operation, 86.7% of patients with actual invasion of trachea and 85.7% of patients with actual invasion of other parts were in accordance with the esophageal activity examination results. Conclusion    Esophageal activity examination under X-ray fluoroscopy can accurately predict the resectability of upper thoracic esophageal cancer, which is a useful supplement to chest CT examination, especially in the aspect of judging the relationship between lesions and the trachea.

Three new labdane diterpenoids, leojaponicone A (1), isoleojaponicone A (2) and methylisoleojaponicone A (3), were isolated from the herb of Leonurus japonicus. The chemical structures of these secondary metabolites were elucidated on the basis of 1D and 2D NMR, including HMQC, and HMBC spectroscopic techniques. All the new compounds were tested in vitro for their acetylcholinesterase and α-glucosidase inhibitory activity. Compounds 1-3 exhibited low inhibitory effects on α-glucosidase with respect to acarbose and exhibited high inhibitory effects on acetylcholinesterase with respect to huperzine A.
Acetylcholinesterase ; metabolism ; Cholinesterase Inhibitors ; chemistry ; isolation & purification ; pharmacology ; Diterpenes ; chemistry ; isolation & purification ; pharmacology ; Glycoside Hydrolase Inhibitors ; chemistry ; isolation & purification ; pharmacology ; Leonurus ; chemistry ; Magnetic Resonance Spectroscopy ; Molecular Structure ; Plant Extracts ; chemistry ; pharmacology

OBJECTIVE: To analyze the clinical phenotype and variant of SLC2A1 gene in a Chinese pedigree affected with glucose transporter type 1 deficiency syndrome (GLUT1-DS). METHODS: Clinical data of a child who was treated due to delayed motor and language development and his family members were collected. DNA was extracted from peripheral blood samples and subjected to high-throughput medical exome sequencing. Candidate variant was verified by Sanger sequencing of his parents and sister. The genotype-phenotype correlation was explored. RESULTS: The child, his mother and sister had common manifestations such as delayed mental and motor development, poor exercise tolerance, easy fatigue and paroxysmal dystonia, but the difference was that the child and his mother had microcephaly and seizures, while his sister did not. A heterozygous missense SLC2A1 c.191T>C (p.L64P) variant was identified in all affected members, which was unreported previously. CONCLUSION The missense SLC2A1 c.191T>C (p.L64P) variant probably underlay the disease in the proband and his mother and sister. Variability of the clinical phenotypes has reflected the genetic and phenotypic diversity of GLUT1-DS. Detection of the novel variant has enriched the spectrum of GLUT1-DS mutations.
Carbohydrate Metabolism, Inborn Errors ; China ; Glucose Transporter Type 1/genetics* ; Humans ; Monosaccharide Transport Proteins/deficiency* ; Mutation ; Pedigree ; Phenotype

OBJECTIVETo explore the efficacy and the security of laparoscopic technique in patients with traumatic colorectal perforation.

METHODSClinical data of 42 patients with traumatic colorectal perforation in our hospital during March of 2005 to March of 2013 were retrospectively analyzed. Twenty-two patients received traditional open operation, and 20 patients received laparoscopic treatment.

RESULTSAs compared to open group, laparoscopic group had shorter operation time [(72 ± 36) min vs. (128 ± 52) min], less blood loss during operation [(21.2 ± 3.6) ml vs. (62.6 ± 8.8) ml], faster postoperative bowel function return[(1.7 ± 0.6) d vs. (3.5 ± 1.2) d], shorter hospital stay after operation [(7.3 ± 1.8) d vs. (13.6 ± 3.9) d], and lower incidence of postoperative complication [35.0% (7/20) vs. 72.7% (16/22)]. The differences between two were statistically significant (all P<0.05).

CONCLUSIONLaparoscopic operation has advantages in the treatment of traumatic colorectal perforation with short operation time, small trauma, rapid recovery and low incidence of complications.

Humans ; Intestinal Perforation ; surgery ; Laparoscopy ; Length of Stay ; Postoperative Complications ; Postoperative Period ; Retrospective Studies