1.Local recurrence of a parosteal osteosarcoma 21 years after incomplete resection.
Andrés COMBALIA ; Ernesto MUÑOZ-MAHAMUD ; Antonio PALACÍN ; Jaume POMÉS ; Vicente LÓPEZ
Chinese Journal of Cancer 2011;30(12):861-866
Parosteal osteosarcoma (POS) is the most common form of surface osteosarcoma. Its symptoms are insidious and its duration prior to diagnosis is considerably longer than that of other types of osteosarcoma. We report a case of POS with a growing mass but no evidence of metastasis. This tumor, which was diagnosed as calcified hematoma with benign characteristics, was incompletely resected in our hospital 21 years before the diagnosis of recurrence. The patient underwent a wide en bloc resection in our hospital and was free of symptoms, with no signs of tumor recurrence or metastasis during a 53-month follow-up.
Adult
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Bone Neoplasms
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diagnosis
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pathology
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surgery
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Diagnostic Errors
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Follow-Up Studies
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Humans
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Humerus
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Magnetic Resonance Imaging
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Male
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Neoplasm Recurrence, Local
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Osteosarcoma, Juxtacortical
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diagnosis
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pathology
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surgery
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Radiopharmaceuticals
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Technetium Tc 99m Medronate
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Tomography, Emission-Computed
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Tomography, X-Ray Computed
2.Comparative study of acute in vitro and short-term in vivo triiodothyronine treatments on the contractile activity of isolated rat thoracic aortas
Ruth Mery LÓPEZ ; Jorge Skiold LÓPEZ ; Jair LOZANO ; Héctor FLORES ; Rosa Angelica CARRANZA ; Antonio FRANCO ; Enrique Fernando CASTILLO
The Korean Journal of Physiology and Pharmacology 2020;24(4):339-348
We aimed to characterize the participation of rapid non-genomic and delayed non-genomic/genomic or genomic mechanisms in vasoactive effects to triiodothyronine (T3), emphasizing functional analysis of the involvement of these mechanisms in the genesis of nitric oxide (NO) of endothelial or muscular origin. Influences of in vitro and in vivo T3 treatments on contractile and relaxant responsiveness of isolated rat aortas were studied. In vivo T3-treatment was 500 μg·kg–1·d–1, subcutaneous injection, for 1 (T31d) and 3 (T33d) days. In experiments with endothelium- intact aortic rings contracted with phenylephrine, increasing concentrations of T3 did not alter contractility. Likewise, in vitro T3 did not modify relaxant responses induced by acetylcholine or sodium nitroprusside (SNP) nor contractile responses elicited by phenylephrine or angiotensin II in endothelium-intact aortas. Concentration- response curves (CRCs) to acetylcholine and SNP in endothelium-intact aortic rings from T31d and T33d rats were unmodified. T33d, but not T31d, treatment diminished CRCs to phenylephrine in endothelium-intact aortic rings. CRCs to phenylephrine remained significantly depressed in both endothelium-denuded and endothelium- intact, nitric oxide synthase inhibitor-treated, aortas of T33d rats. In endotheliumdenuded aortas of T33d rats, CRCs to angiotensin II, and high K+ contractures, were decreased. Thus, in vitro T3 neither modified phenylephrine-induced active tonus nor CRCs to relaxant and contractile agonists in endothelium-intact aortas, discarding rapid non-genomic actions of this hormone in smooth muscle and endothelial cells. Otherwise, T33d-treatment inhibited aortic smooth muscle capacity to contract, but not to relax, in an endothelium- and NO-independent manner. This effect may be mediated by delayed non-genomic/genomic or genomic mechanisms.
3.Comparative study of acute in vitro and short-term in vivo triiodothyronine treatments on the contractile activity of isolated rat thoracic aortas
Ruth Mery LÓPEZ ; Jorge Skiold LÓPEZ ; Jair LOZANO ; Héctor FLORES ; Rosa Angelica CARRANZA ; Antonio FRANCO ; Enrique Fernando CASTILLO
The Korean Journal of Physiology and Pharmacology 2020;24(4):339-348
We aimed to characterize the participation of rapid non-genomic and delayed non-genomic/genomic or genomic mechanisms in vasoactive effects to triiodothyronine (T3), emphasizing functional analysis of the involvement of these mechanisms in the genesis of nitric oxide (NO) of endothelial or muscular origin. Influences of in vitro and in vivo T3 treatments on contractile and relaxant responsiveness of isolated rat aortas were studied. In vivo T3-treatment was 500 μg·kg–1·d–1, subcutaneous injection, for 1 (T31d) and 3 (T33d) days. In experiments with endothelium- intact aortic rings contracted with phenylephrine, increasing concentrations of T3 did not alter contractility. Likewise, in vitro T3 did not modify relaxant responses induced by acetylcholine or sodium nitroprusside (SNP) nor contractile responses elicited by phenylephrine or angiotensin II in endothelium-intact aortas. Concentration- response curves (CRCs) to acetylcholine and SNP in endothelium-intact aortic rings from T31d and T33d rats were unmodified. T33d, but not T31d, treatment diminished CRCs to phenylephrine in endothelium-intact aortic rings. CRCs to phenylephrine remained significantly depressed in both endothelium-denuded and endothelium- intact, nitric oxide synthase inhibitor-treated, aortas of T33d rats. In endotheliumdenuded aortas of T33d rats, CRCs to angiotensin II, and high K+ contractures, were decreased. Thus, in vitro T3 neither modified phenylephrine-induced active tonus nor CRCs to relaxant and contractile agonists in endothelium-intact aortas, discarding rapid non-genomic actions of this hormone in smooth muscle and endothelial cells. Otherwise, T33d-treatment inhibited aortic smooth muscle capacity to contract, but not to relax, in an endothelium- and NO-independent manner. This effect may be mediated by delayed non-genomic/genomic or genomic mechanisms.
4.Trabectedin therapy as an emerging treatment strategy for recurrent platinum-sensitive ovarian cancer.
José Antonio LÓPEZ-GUERRERO ; Ignacio ROMERO ; Andrés POVEDA
Chinese Journal of Cancer 2015;34(1):41-49
Epithelial ovarian cancer (OC) is a common gynecologic malignancy in women. The standard treatment for OC is maximal cytoreductive surgical debulking followed by platinum-based chemotherapy. Despite the high response rate to primary therapy, approximately 85% of patients will develop recurrent ovarian cancer (ROC). This review identifies the clinical use of trabectedin in the treatment algorithm for ROC, with specific emphasis on platinum-sensitive ROC, for which trabectedin in combination with pegylated liposomal doxorubicin has been approved as a treatment protocol. The main mechanisms of action of trabectedin at the cellular level and in the tumor microenvironment is also discussed as bases for identifying biomarkers for selecting patients who may largely benefit from trabectedin-based therapies.
Antineoplastic Agents, Alkylating
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therapeutic use
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Clinical Trials as Topic
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DNA Damage
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Dioxoles
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administration & dosage
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pharmacology
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therapeutic use
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Doxorubicin
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administration & dosage
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analogs & derivatives
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Female
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Humans
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Neoplasm Recurrence, Local
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drug therapy
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Neoplasms, Glandular and Epithelial
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drug therapy
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Ovarian Neoplasms
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drug therapy
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Polyethylene Glycols
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administration & dosage
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Tetrahydroisoquinolines
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administration & dosage
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pharmacology
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therapeutic use
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Tumor Microenvironment
5.Molecular epidemiology of Aleutian mink disease virus causing outbreaks in mink farms from Southwestern Europe: a retrospective study from 2012 to 2019
Alberto PRIETO ; Ricardo FERNÁNDEZ-ANTONIO ; Gonzalo LÓPEZ-LORENZO ; José Manuel DÍAZ-CAO ; Cynthia LÓPEZ-NOVO ; Susana REMESAR ; Rosario PANADERO ; Pablo DÍAZ ; Patrocinio MORRONDO ; Pablo DÍEZ-BAÑOS ; Gonzalo FERNÁNDEZ
Journal of Veterinary Science 2020;21(4):e65-
Background:
Aleutian mink disease virus (AMDV) causes major economic losses in fur-bearing animal production. The control of most AMDV outbreaks is complex due to the difficulties of establishing the source of infection based only on the available on-farm epidemiological data. In this sense, phylogenetic analysis of the strains present in a farm may help elucidate the origin of the infection and improve the control and biosecurity measures.
Objectives:
This study had the following aims: characterize the AMDV strains from most outbreaks produced at Spanish farms between 2012–2019 at the molecular level, and assess the utility of the combined use of molecular and epidemiological data to track the possible routes of infection.
Methods:
Thirty-seven strains from 17 farms were partially sequenced for the NS1 and VP2 genes and analyzed phylogenetically with other strains described worldwide.
Results:
Spanish AMDV strains are clustered in four major clades that generally show a good geographical correlation, confirming that most had been established in Spain a long time ago. The combined study of phylogenetic results and epidemiological information of each farm suggests that most of the AMDV outbreaks since 2012 had been produced by within-farm reservoirs, while a few of them may have been due to the introduction of the virus through international trade.
Conclusions
The combination of phylogenetic inference, together with epidemiological data, helps assess the possible origin of AMDV infections in mink farms and improving the control and prevention of this disease.
6.Analysis of Workplace Accidents in Automotive Repair Workshops in Spain.
Antonio LÓPEZ-ARQUILLOS ; Juan Carlos RUBIO-ROMERO
Safety and Health at Work 2016;7(3):231-236
BACKGROUND: To analyze the effects of the factors associated with different types of injury (superficial wounds, dislocations and sprains, bone fractures, concussion and internal injuries, burns scalding and freezing) caused by occupational accidents in automotive repair workshops. METHODS: Study of a sample consisting of 89,954 industry accidents reported from 2003 to 2008. Odds ratios were calculated with a 95% confidence interval. RESULTS: Belonging to a small company is a risk factor for suffering three of the five types of injury studied. Women are less likely to suffer burns and superficial wounds, and more likely to suffer dislocations or sprains. Foreign workers are more likely to suffer concussion and internal injuries. CONCLUSION: Health and safety strategies and accident prevention measures should be individualized and adapted to the type of worker most likely to be injured in each type of accident. Occupational health and safety training courses designed according to worker profile, and improving the participation of the workers in small firms creating regional or roving safety representatives would improve working conditions.
Accident Prevention
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Accidents, Occupational
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Burns
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Dislocations
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Education*
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Female
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Fractures, Bone
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Humans
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Occupational Health
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Odds Ratio
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Risk Factors
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Spain*
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Sprains and Strains
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Wounds and Injuries
7.Intestinal ultrasonography and fecal calprotectin for monitoring inflammation of ileal Crohn’s disease: two complementary tests
José María PAREDES ; Tomás RIPOLLÉS ; Ángela ALGARRA ; Rafael DIAZ ; Nadia MORENO ; Patricia LATORRE ; María Jesús MARTÍNEZ ; Pilar LLOPIS ; Antonio LÓPEZ ; Eduardo MORENO-OSSET
Intestinal Research 2022;20(3):361-369
Background/Aims:
Tight control of inflammation and adjustment of treatment if activity persists is the current strategy for the management of Crohn’s disease (CD). The usefulness of fecal calprotectin (FC) in isolated involvement of the small intestine in CD is controversial. To assess the usefulness of FC to determine the inflammatory activity detected by intestinal ultrasonography (IUS) in ileal CD.
Methods:
Patients with exclusively ileal involvement CD who underwent IUS and an FC were prospectively included. Simple ultrasound index was used to determine inflammatory activity. The usual statistical tests for comparison of diagnostic techniques were used.
Results:
One hundred and five patients were included, IUS showed inflammatory activity in 59% of patients and complications in 18.1%. FC showed a significant correlation with IUS in the weak range (Spearman coefficient r=0.502; P<0.001); the area under the receiver operating characteristic curve was 0.79 (95% confidence interval, 0.70–0.88; P<0.001). The FC value that best reflected the activity in IUS was 100 μg/g with sensitivity, specificity, and positive and negative predictive values of 73.0%, 71.4%, 79.3% and 63.8%, respectively. There were no differences in FC concentration between patients with or without transmural complications. The addition of serum C-reactive protein to FC did not improve the ability to assess IUS activity.
Conclusions
FC has a significant correlation with IUS to monitor ileal CD activity. This correlation is weak and it does not allow assessing the presence of CD complications. Both tests should be used in conjunction for tight control of ileal CD. More studies on noninvasive tests in this location are needed.
8.Characterization of hypotensive and vasorelaxant effects of PHAR-DBH-Me a new cannabinoid receptor agonist
Oscar Alberto LÓPEZ-CANALES ; Natalia PAVÓN ; Laura Matilde UBALDO-REYES ; Marco Antonio JUÁREZ-OROPEZA ; Patricia Victoria TORRES-DURÁN ; Ignacio REGLA ; María Cristina PAREDES-CARBAJAL
The Korean Journal of Physiology and Pharmacology 2022;26(2):77-86
The effect of PHAR-DBH-Me, a cannabinoid receptor agonist, on different cardiovascular responses in adult male rats was analyzed. The blood pressure was measured directly and indirectly. The coronary flow was measured by Langendorff preparation, and vasomotor responses induced by PHAR-DBH-Me in aortic rings precontracted with phenylephrine (PHEN) were analyzed. The intravenous injection of the compound PHAR-DBH-Me (0.018–185 µg/kg) resulted in decreased blood pressure; maximum effect was observed at the dose of 1,850 µg/kg. A concentrationdependent increase in the coronary flow was observed in a Langendorff preparation. In the aortic rings, with and without endothelium, pre-contracted with PHEN (10–6 M), the addition of PHAR-DBH-Me to the superfusion solution (10–12 –10 –5 M), produced a vasodilator response, which depends on the concentration and presence of the endothelium. L-NAME inhibited these effects. Addition of CB 1 receptor antagonist (AM 251) did not modify the response, while CB2 receptor antagonist (AM630) decreased the potency of relaxation elicited by PHAR-DBH-Me. Indomethacin shifted the curve concentration-response to the left and produced an increase in the magnitude of the maximum endothelium dependent response to this compound. The maximum effect of PHAR-DBH-Me was observed with the concentration of 10 –5 M. These results show that PHAR-DBH-Me has a concentration-dependent and endothelium-dependent vasodilator effect through CB2 receptor. This vasodilation is probably mediated by the synthesis/release of NO. On the other hand, it is suggested that PHAR-DBH-Me also induces the release of a vasoconstrictor prostanoid.
9.Mediterranean Diet and Physical Activity Protect from Silent Brain Infarcts in a Cohort of Patients with Atrial Fibrillation
Irene ESCUDERO-MARTÍNEZ ; Fernando MANCHA ; Angela VEGA-SALVATIERRA ; María Irene AYUSO ; Rafael F OCETE ; Pilar ALGABA ; Antonio LÓPEZ-RUEDA ; Pilar PIÑERO ; Elena FAJARDO ; José Román FERNÁNDEZ-ENGO ; Eva María MARTÍN-SÁNCHEZ ; Alejandro GALVAO-CARMONA ; Elena ZAPATA-ARRIAZA ; Lucía LEBRATO ; Blanca PARDO-GALIANA ; Juan Antonio CABEZAS ; Alejandro GONZÁLEZ ; Francisco MONICHE ; Joan MONTANER
Journal of Stroke 2019;21(3):353-355
No abstract available.
Atrial Fibrillation
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Brain
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Cohort Studies
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Diet, Mediterranean
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Humans
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Motor Activity
10. Polyphenolic content, in vitro antioxidant activity and chemical composition of extract from Nephelium lappaceum L. (Mexican rambutan) husk
Cristian HERNÁNDEZ ; Juan ASCACIO-VALDÉS ; Heliodoro DE LA GARZA ; Antonio AGUILERA-CARBÓ ; Jorge WONG-PAZ ; Cristóbal Noé AGUILAR ; Guillermo Cristian MARTÍNEZ-ÁVILA ; Cecilia CASTRO-LÓPEZ
Asian Pacific Journal of Tropical Medicine 2017;10(12):1201-1205
Objective To determinate the recovery of total polyphenolic compounds content, in vitro antioxidant activity and HPLC/ESI/MS characterization of extract from Nephelium lappaceum L. (Mexican rambutan). Methods The rambutan husk extract was obtained by aqueous extraction and a polyphenolic fraction was recovered using Amberlite XAD-16. The total polyphenolic compounds content was determined by the Folin Ciocalteu and butanol-HCI methods. In vitro antioxidant activity was performed using ABTS and ferric reducing antioxidant power methods. Results Mexican rambutan husk showed a total polyphenolic content of 582 mg/g and an evident antioxidant activity by ABTS and ferric reducing antioxidant power analysis. The HPLC/ESI/MS assay allowed the identification of 13 compounds, most of which belong to ellagitannins. Geraniin, corilagin and ellagic acid were present in the sample; the mineral composition was also evaluated. Conclusions Rambutan husk cultivated in Mexico is a promising source for the recovery of added value bioactive compounds with antioxidant activity, which have potential applications as bioactive antioxidant agents for the treatment of diseases.