1.Frequency of Js/a, Js/b, Kp/a, Kp/b, M/g and Xga/ blood group antigens among Koreans.
Seok Lae CHAE ; Kyou Sup HAN ; Han Il CHO ; Sang In KIM
Korean Journal of Blood Transfusion 1991;2(1):69-72
No abstract available.
Blood Group Antigens*
4.Identification and Molecular Biology of Variant D Blood Group of RHD*95A Genotype.
Xin LIU ; Lian-Hui WANG ; Zi-Heng XU ; Jin SHU ; Meng-Yuan DONG ; Xiao-Yan TONG ; Xiu-Yun XU
Journal of Experimental Hematology 2022;30(6):1839-1844
OBJECTIVE:
To explore the molecular biology of D variant blood group with RHD*95A genotype and the genetic mechanism of its generation.
METHODS:
A total of 6 samples from 3 generations of a family were analyzed. RHD blood group was identified by saline test tube and microcolumn gel card method. 10 exons of RHD gene were amplified by Polymerase Chain Reaction-Sequence Specific Primer (PCR-SSP) and analyzed by direct sequencing. Homology modeling was used to compare the structural differences between mutant RHD protein and wild-type RHD protein.
RESULTS:
The proband was identified as D variant by serological identification, RHD gene sequencing directly detected a c. 95 c > A mutation in exon 1 that leads to encoding the 32-bit amino acids by threonine Thr (T) into aspartic acid Asn (N), the rest of the exon sequences were normal compared with the normal RHD*01 gene. In the family, the proband's father, grandmather and uncle were all carried the same RHD*95A allele. Protein modeling results suggested that the hydrogen chain connected to the 32nd amino acid residue was changed after p.T32N mutation, which affected the structural stability of RHD protein.
CONCLUSION
The first genetic lineage of the RHD*95A gene was identified in a Chinese population. The c.95C>A mutation in RHD gene was found in the family, which resulted in reduced expression of RHD antigen and showed D variant, the mutation could be stably inheritable. Gene identification and protein structure analysis of D variant population is helpful to explore the molecular mechanism of its formation and ensure the safety of blood transfusion.
Humans
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Blood Group Antigens
5.The distribution of red cell blood group antigens and alloantibodies in surgical transfusion
Journal of Medical and Pharmaceutical Information 2004;0(2):24-26
Objective: Study some red cell blood group antigens and allo-antibodies of blood donors and surgical patients at Viet Duc Hospital order to contribute to safe blood transfusion. Subjects: 122 blood donors were tested antigens of red blood cell: Rh, Kell, Duffy, Lutheran, MNS, Lewis, P... with anti-serum developed by Gamma Company, 393 blood donors were undergone Mill test with anti-Mia, 938 blood donors and 1036 surgical patients were tested by Polybrene method for allo-antibody. Results: frequencies of red blood cell antigens out of ABO system of 122 blood donors were listed in Table 1. Prevalence of Mill antigen in 393 blood donors was 9.9%. Frequencies of allo-antibody were 0% and <1% in 938 blood donors group and surgical patients group, respectively. Among 93 surgical patients had multi-transfusion, frequency of allo-antibody was 4.3%
Antigens
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Isoantibodies
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Blood Transfusion
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surgery
6.Genotyping of the Platelet Alloantigens by Reverse Dot Blot Hybridization.
Journal of the Korean Medical Association 1997;40(4):507-512
No abstract available.
Antigens, Human Platelet*
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Blood Platelets*
7.Influence of "dosage effect" on unexpected antibody identification.
Xiao-Lin SUN ; Yang YU ; Xiao-Zhen GUAN ; Lin-Feng CHEN ; De-Qing WANG
Journal of Experimental Hematology 2015;23(1):222-227
OBJECTIVEThis study was to investigate the influence of "dosage effect" on unexpected antibody identification and explore its condition, scope and regularity.
METHODSA total of 40 blood recipient samples containing definite unexpected antibodies were selected by column agglutination technology, then AB fresh plasma was used to dilute the samples to obtain different concentrate liquid. After selecting panel cells which show positive with corresponding unexpected antibody in the serum, "single dosage" antigens were distinguished from "double dosage" ones, and then the antigen-antibody reactions were observed between "single dosage" panel cells and respective diluted recipient samples (by column agglutination technology). It's believable that the highest concentration which retains a negative result was choose to evaluate the agglutination strength between "double dosage" panel cells and diluted unexpected antibody, and to observe the difference happened at different "dosage" antigens with unexpressed antibody.
RESULTSAmong 40 diluted recipient samples detected by column agglutination technology, the "dosage effect" appeared in 31 diluted samples. There were 30 samples in which the unexpected antibody agglutinated "double dosage" antigens ≤ 2+, while "single dosage" antigens negative. It appeared in another 1 diluted sample, in which the unexpected antibody agglutinated "double dosage" antigens 3+. There were 9 diluted samples in which the unexpected antibody agglutinated panel cells showing negative results (strength was between 1+-3+ before dilution).
CONCLUSIONSWhen the unexpected antibodies in Rh, MNS, Kidd, Duffy agglutinated "double dosage" antigens ≤ 2+ (by column agglutination technology) , "single dosage" antibody reaction maybe weaken, even be negative, and it may cause the "dosage effect" to interfere the unexpected antibody identification. The "dosage effect" appears in Rh, MNS, Kidd, Duffy blood system usually.
Antibodies ; Antigens ; Blood Transfusion ; Humans
8.Phenotyping human platelet antigens of the Kinh population in the centre, Vietnam
Journal of Medical Research 2004;27(1):1-5
Located on the platelet membrane surface, human platelet specific antigens (HPAs) are related to alloimmune thrombocytopenic syndromes. The frequencies of HPA genes vary between different populations. In this study, the author initially identified HPA gene frequencies of Kinh population which are living in the Centre of Vietnam by polymerase chain reaction with specific sequence primers (PCR-SSP). The frequencies observed are following: 0.977, 0.023, 0.000 for HPA-1a/a, HPA-1b/b; 0.827, 0.161, 0.011 for HPA-2a/a, HPA-2a/b, HPA-2b/b; 0.276, 0.552, 0.173 for HPA-3a/a, HPA-3a/b, HPA-3b/b; 0.920, 0.080, 0.000 for HPA-5a/a, HPA-5a/b, HPA-5b/b. respectively. For HPA-4, all 87 donor samples presented HPA-4a/a
Antigens, Human Platelet
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Blood Platelets
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blood
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Vietnam
9.Evaluation of renal transplantation results at Hue Central Hospital
Journal of Practical Medicine 2005;510(4):51-54
Study on 12 transplanted couples at Hue Central Hospital from 2001 to now. Transplanted selections were based on the compatibility of blood groups and HLA (more than 3/8). Anti-interleukin 2 was used in cases of HLA compatibility of 3/6 to help expanding the selections of donor-receiver. Transplanted patient who had positive HbsAg but no symptoms, normal level of serum liver enzymes, can be transplanted and carefully followed up post-operative serum liver enzymes. Gradually decrease doses of corticoid therapy could help to limit side-effects of corticoid.
Kidney Transplantation
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Blood Group Antigens
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Blood
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Epidemiology
10.The Cell Surface Antigen A,B,O(H) as An Indicator of Malignant Potential in Bladder Carcinoma: A Preliminary Report.
Young Won CHUNG ; Tai Chin KIM
Korean Journal of Urology 1982;23(7):881-887
Currently, the cell surface antigen A,B,O(H) is thought to be an important indicator of malignant potential in bladder carcinoma. Herein, we performed SRCA test in 54 bladder carcinoma for detection of such an isoantigen, comparing the SRCA result to its tumor grade and stage. Also, various significances including the clinical application of SRCA test for the management of the bladder carcinoma were discussed. The results were as follows: 1. Of 54 patients, 34 patients were low stage(0-A) and low grade(1-2). 2. There is a significant correlation between tumor grade and SRCA test: Of 38 patients with low grade. 19 patients were SRCA positive, but of 16 patients with high grade. all were SRCA negative. 3. There is a significant correlation between tumor stage and SRCA test: Of 36 patients with low stage, 18 patients were SRCA positive, but of 18 patients with high stage(above B1), only one patient was SRCA positive. 4. There is a high possibility of false-negative results in detecting O(H) isoantigen: Of 36 patients with low stage, 6 patients were blood group 0 who were all SRCA negative. but 30 patients with other blood groups showed variable SRCA results. 5. There is a considerable correlation between tumor recurrence and SRCA result: Of 20 patients who were followed more than one year after initial TUR, 8 patients were SRCA positive, of these 4 patients were recurred, but 9 patients of 12 patients with SRCA negative were recurred.
Antigens, Surface*
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Blood Group Antigens
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Humans
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Isoantigens
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Recurrence
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Urinary Bladder*