1.Expression of dendritic cell markers on cultured neutrophils and its modulation by anti-apoptotic and pro-apoptotic compounds.
Hae Young PARK ; Jun O JIN ; Min Gyu SONG ; Joo In PARK ; Jong Young KWAK
Experimental & Molecular Medicine 2007;39(4):439-449
Neutrophils are also known to acquire the characteristics of dendritic cells (DCs) under the appropriate conditions. In this study, neutrophils were cultivated in vitro in the presence or absence of compounds modulating their survival in an attempt to characterize the expression profile of the DC markers. Higher MHC-II, CD80, CD86, CD83, and CD40 expression levels were detected on the surface of the cultured neutrophils for 24 h than on the freshly isolated cells. The annexin V-positive cells showed a higher expression level of the DC markers than the annexin V-negative cells. The population of neutrophils double stained with annexin V and the DC markers increased after being incubated with agonistic anti-Fas Ab. LPS, the anti-apoptotic compound, decreased the CD86 and MHC-II expression levels but 50-60% of the DC marker-positive cells were detected in the annexin V-positive cells. In contrast, CD80, CD86, CD83, and HLA-DR mRNA levels increased in the GM-CSF-treated neutrophils but not in the anti-Fas Ab-treated neutrophils. T cell proliferation was inhibited by co-culturing them with anti-Fas Ab- or LPS-treated neutrophils at a high neutrophil:T cell ratio. However, the superantigen-mediated T cell proliferation was increased by the LPS-treated neutrophils but decreased by the anti-Fas Ab-treated neutrophils. There was a lower level of interferon-gamma production in the T cells co-cultured with anti-Fas Ab-treated neutrophils than with the LPS-treated neutrophils. This suggests that apoptotic neutrophils express DC markers on their surface and the differential expression of DC markers might have a detrimental effect on the immune reaction.
Antigen Presentation
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Antigens, CD/biosynthesis
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Antigens, CD95/pharmacology
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Antigens, Differentiation/*biosynthesis
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*Apoptosis
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Cells, Cultured
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Dendritic Cells/*metabolism
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Humans
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Lipopolysaccharides/pharmacology
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Lymphocyte Activation
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Neutrophils/*metabolism/physiology
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T-Lymphocytes/immunology
2.Sensitivity of CD95-induced apoptosis in different proliferative status of human retinal pigment epithelial cells.
Jin Hee CHANG ; Se Woong KANG ; Don Il HAM
Korean Journal of Ophthalmology 2001;15(2):74-80
It is known that CD95 (APO-1/Fas) is expressed on the cell surface, and apoptotic cell death can be induced by the CD95 ligation in the cultured, proliferating human retinal pigment epithelial (RPE) cells. However, little is known about CD95 on the non-proliferating RPE cells. In this study, human RPE cells were cultured up to 4 weeks after they reached the confluence, to simulate the non-proliferating RPE cells in situ. There was no significant difference in CD95 expression on the cell surface between the predominantly proliferating, preconfluent cells and predominantly non-proliferating, postconfluent cells in flow cytometric assays. However, unlike proliferating cells, no cellular death occurred in the predominantly non-proliferating cells after the treatment of agonistic anti-CD95 antibody with cycloheximide, pretreated with interferon-gamma. Our results suggest that the CD95/CD95L system probably plays a physiologic role in vivo to remove the abnormal, proliferating RPE cells, and factors other than the surface expression of CD95 may determine the sensitivity to the CD95 signals.
Antigens, CD95/*pharmacology
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Apoptosis/*physiology
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Cells, Cultured
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Human
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Pigment Epithelium of Eye/cytology/*drug effects/*physiology
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Sensitivity and Specificity
3.Spontaneous programmed cell death of peripheral blood mononuclear cells from HIV-infected persons is decreased with interleukin-15.
Kyung Hee CHANG ; June Myung KIM ; Hyo Youl KIM ; Young Goo SONG ; Young Hwa CHOI ; Yoon Soo PARK ; Jung Ho CHO ; Sung Kwan HONG
Yonsei Medical Journal 2000;41(1):112-118
Interleukin 15 (IL-15) is an important regulatory cytokine in cellular immunity. In vitro replacement of IL-15 has been shown to enhance immunity in Human immunodeficiency virus type 1 (HIV-1) infected lymphocytes. We evaluated the effect of IL-15 on the survival of peripheral blood mononuclear cells of HIV patients by examining in vitro lymphocyte apoptosis, and correlated the process with Bcl-2 and Fas gene regulation. Peripheral blood mononuclear cells (PBMC) from 21 HIV-infected adults and 24 HIV-seronegative healthy individuals were isolated and cultured to determine the effect of escalating doses of IL-15 (0, 1, 10, 100, 1000 ng/mL) on apoptosis. Lymphocyte proliferation assay with (3H) TdR was measured and Bcl-2 and Fas gene regulation was observed. The results were as follows: 1) IL-15 reduced culture induced lymphocyte apoptosis in HIV patients in a dose dependent manner, and reached a plateau level at a concentration of 100 ng/ml; 2) IL-15 significantly reduced the level of apoptosis after 3 days (14%) and 5 days (15%) of culture in HIV patients, while no difference was observed in HIV (-) donors; 3) The percentage of viable cells among the total number of lymphocytes was significantly enhanced by 25% in HIV patients with IL-15; 4) Bcl-2 expression was decreased in HIV patients (53.9 +/- 12.3%) compared to HIV (-) donors (93.0 +/- 3.7%), and IL-15 increased Bcl-2 expression by 21.2 +/- 5.2% in HIV patients; 5) Fas expression was increased in HIV patients (70.2 +/- 4.6%) compared to HIV (-) donors (32.4 +/- 4.3%), and IL-15 increased Fas expression by 8.4 +/- 1.2% in HIV (-) donors. Our findings indicate that IL-15 may influence immunologic abnormalities in HIV infection, particularly its ability to prevent apoptosis of lymphocytes by suppressing the down-modulation of Bcl-2. This may provide an experimental basis for IL-15 immunotherapy.
Antigens, CD95/genetics
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Apoptosis/drug effects*
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Cells, Cultured
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Gene Expression Regulation/physiology
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Genes, bcl-2/genetics
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HIV Infections/blood*
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Human
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Interleukin-15/pharmacology*
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Monocytes/drug effects*
4.Changes of phospholipase D activity in TNF-alpha and anti-Fas/Apo1 monoclonal antibody induced apoptosis in HL-60 and A20 cells.
Jin Ho KANG ; In Cheol SHIN ; Joong Soo HAN
Experimental & Molecular Medicine 1998;30(1):21-27
The changes of phospholipase D (PLD) activity were investigated during the courses of apoptotic process induced by tumor necrosis factor (TNF)-alpha or anti-Fas/Apo1 antibody in human premyelocyte HL-60 and murine B cell lymphoma A20 cells. The treatment of recombinant TNF-alpha to HL-60 cells resulted in the increased PLD activity as determined by the phosphatidylethanol formation in the presence of 1% ethanol. The enhancement of PLD activity was also observed in the anti-Fas/Apo1 monoclonal antibody-treated A20 cells. However, the activity of PLD was maximized when HL-60 and A20 cells were treated with either TNF-alpha or anti-Fas/Apo1 monoclonal antibody for 6 h. Both TNF-alpha and anti-Fas/Apo1 monoclonal antibody increased PLD activity in a dose-dependent manner up to 200 U/ml and 200 ng/ml, respectively. When the intracellular activity of protein kinase C (PKC) was interrupted by treatment of calphostin-C, both the PLD activation and the apoptosis induced by TNF-alpha and anti-Fas/Apo1 monoclonal antibody appeared to be inhibited. Since PKC is reported to activate PLD, the results indicate that the intracellular signaling cascade via PLD may play a role in the induction of apoptosis induced by TNF-alpha and anti-Fas/Apo1 monoclonal antibody.
Animal
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Antibodies, Monoclonal/pharmacology
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Antigens, CD95/metabolism*
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Antigens, CD95/immunology
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Apoptosis*
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DNA Fragmentation
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Dose-Response Relationship, Drug
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Enzyme Activation
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HL-60 Cells
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Human
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Leukemia, Promyelocytic, Acute
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Lymphoma, B-Cell
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Mice
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Naphthalenes/pharmacology
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Phospholipase D/metabolism*
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Protein Kinase C/antagonists & inhibitors
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Receptors, Tumor Necrosis Factor/metabolism*
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Signal Transduction
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Tumor Necrosis Factor/pharmacology*
5.Metabolic loading of guanosine induces chondrocyte apoptosis via the Fas pathway.
Dong Jo KIM ; Jun Ho CHUNG ; Eun Kyeong RYU ; Jung Hyo RHIM ; Yoon Sic RYU ; So Hyun PARK ; Kyung Tae KIM ; Heun Soo KANG ; Hong Keun CHUNG ; Sang Chul PARK
Experimental & Molecular Medicine 2006;38(4):401-407
Although the apoptosis of chondrocytes plays an important role in endochondral ossification, its mechanism has not been elucidated. In this study, we show that guanosine induces chondrocyte apoptosis based on the results of acridine orange/ ethidium bromide staining, caspase-3 activation, and sub-G1 fraction analysis. The potent inhibitory effect of dipyridamole, a nucleoside transporter blocker, indicates that extracellular guanosine must enter the chondrocytes to induce apoptosis. We found that guanosine promotes Fas-Fas ligand interaction which, in turn, leads to chondrocyte apoptosis. These findings indicate a novel mechanism for endochondral ossification via metabolic regulation.
Tumor Necrosis Factors/metabolism
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Signal Transduction/drug effects
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Receptors, Tumor Necrosis Factor/*metabolism
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Rats, Sprague-Dawley
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Rats
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Nucleoside Transport Proteins/metabolism
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Membrane Glycoproteins/metabolism
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Guanosine/*pharmacology/physiology
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Fas Ligand Protein
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Chondrocytes/*drug effects/metabolism
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Apoptosis/*drug effects
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Antigens, CD95
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Animals
6.-Melanocyte Stimulating Hormone (MSH) decreases cyclosporine A induced apoptosis in cultured human proximal tubular cells.
Sang Kyung JO ; So Young LEE ; Sang Youp HAN ; Dae Ryong CHA ; Won Yong CHO ; Hyoung Kyu KIM ; Nam Hee WON
Journal of Korean Medical Science 2001;16(5):603-609
The pathogenesis of chronic cyclosporine A (CsA) nephrotoxicity has not been elucidated, but apoptosis is thought to play an important role in CsA induced tubular atrophy. Recently Fas-Fas ligand system mediated apoptosis has been frequently reported in many epithelial cells as well as in T lymphocytes. We investigated the ability of CsA to induce apoptosis in cultured human proximal tubular epithelial cells and also the effect of -MSH on them. Fas, Fas ligand, and an intracellular adaptor protein, Fas-associating protein with death domain (FADD) expression, and poly-ADP ribose polymerase (PARP) cleavage were also studied. CsA induced apoptosis in cultured tubular epithelial cells demonstrated by increased number of TUNEL positive cells and it was accompanied by a significant increase in Fas mRNA and Fas ligand protein expressions. FADD and the cleavage product of PARP also increased, indicating the activation of caspase. In -MSH co-treated cells, apoptosis markedly decreased with downregulation of Fas, Fas ligand and FADD expressions and also the cleavage product of PARP. In conclusion, these data suggest that tubular cell apoptosis mediated by Fas system may play a role in tubular atrophy in chronic CsA nephrotoxicity and pretreatment of -MSH may have a some inhibitory effect on CsA induced tubular cell apoptosis.
Antigens, CD95/genetics
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Apoptosis/*drug effects
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Carrier Proteins/biosynthesis
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Caspases/physiology
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Cells, Cultured
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Cyclosporine/*toxicity
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Human
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Immunosuppressive Agents/*toxicity
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Kidney Tubules, Proximal/cytology/*drug effects/metabolism
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Membrane Glycoproteins/biosynthesis
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NAD+ ADP-Ribosyltransferase/metabolism
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RNA, Messenger/analysis
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alpha-MSH/*pharmacology
7.Effect of retinoic acid on apoptosis and expression of Fas proteins in mouse blastocysts cultured in vitro.
Yan'e, XIONG ; Duanlian, ZHANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2008;28(3):239-42
Mouse blastocysts were exposed to doses of 0, 1 and 10 mumol/L retinoic acid (RA) for 24 h and the cytotoxic effect of RA on the mouse blastocysts in vitro was observed. FITC-labeled terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL-FITC) assay was employed to stain apoptotic cells and immunohistochemical S-P staining method was used to detect the expression of Fas protein in mouse blastocysts in vitro. The results showed that RA could induce apoptosis and increase the expression of Fas proteins of trophectoderm (TE) and inner cell mass (ICM) cells in blastocysts. Compared with the findings for the control blastocysts, exposure to RA (10 mumol/L) resulted in a more significant apoptosis and higher expression level of Fas proteins (P<0.01). It was concluded that RA could induce apoptosis, which may result in a significant reduction in the average number of total cells and the trophectoderm/inner cell mass in blastocysts and an increased expression of Fas protein, suggesting that RA had a cytotoxic effect on the growth and development of early embryos in mice.
Antigens, CD95/*biosynthesis
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Apoptosis/*drug effects
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Blastocyst/cytology
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Blastocyst/*metabolism
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Cell Culture Techniques/methods
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Cells, Cultured
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Gene Expression Regulation/*drug effects
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In Situ Nick-End Labeling
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RNA, Messenger/metabolism
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Tretinoin/*pharmacology
8.D60-sensitive tyrosine phosphorylation is involved in Fas-mediated phospholipase D activation.
Jong Gon KIM ; In Cheol SHIN ; Ki Sung LEE ; Joong Soo HAN
Experimental & Molecular Medicine 2001;33(4):303-309
Both Fas and PMA can activate phospholipase D via activation of protein kinase Cbeta in A20 cells. Phospholipase D activity was increased 4 fold in the presence of Fas and 2.5 fold in the presence of PMA. The possible involvement of tyrosine phosphorylation in Fas-induced activation of phospholipase D was investigated. In five minute after Fas cross-linking, there was a prominent increase in tyrosine phosphorylated proteins, and it was completely inhibited by D609, a specific inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC). A tyrosine kinase inhibitor, genistein, can partially inhibit Fas-induced phospholipase D activation. There were no effects of genistein on Fas-induced activation of PC-PLC and protein kinase C. These results strongly indicate that tyrosine phosphorylation may in part account for the increase in phospholipase D activity by Fas cross-linking and D609 can block not only PC-PLC activity but also tyrosine phosphorylation involved in Fas-induced phospholipase D activation.
Animal
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Antibodies, Monoclonal/immunology/*pharmacology
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Antigens, CD95/immunology/*metabolism
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Bridged Compounds/*pharmacology
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Cell Line
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Cross-Linking Reagents
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Dose-Response Relationship, Immunologic
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Enzyme Activation
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Genistein/pharmacology
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Hydrolysis
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Lymphoma/pathology
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Mice
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Phospholipase C/*antagonists & inhibitors
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Phospholipase D/*metabolism
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Phosphorylation
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Phosphorylcholine/metabolism
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Solubility
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Thiones/*pharmacology
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Tumor Cells, Cultured
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Tyrosine/*metabolism
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Water/chemistry
9.D60-sensitive tyrosine phosphorylation is involved in Fas-mediated phospholipase D activation.
Jong Gon KIM ; In Cheol SHIN ; Ki Sung LEE ; Joong Soo HAN
Experimental & Molecular Medicine 2001;33(4):303-309
Both Fas and PMA can activate phospholipase D via activation of protein kinase Cbeta in A20 cells. Phospholipase D activity was increased 4 fold in the presence of Fas and 2.5 fold in the presence of PMA. The possible involvement of tyrosine phosphorylation in Fas-induced activation of phospholipase D was investigated. In five minute after Fas cross-linking, there was a prominent increase in tyrosine phosphorylated proteins, and it was completely inhibited by D609, a specific inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC). A tyrosine kinase inhibitor, genistein, can partially inhibit Fas-induced phospholipase D activation. There were no effects of genistein on Fas-induced activation of PC-PLC and protein kinase C. These results strongly indicate that tyrosine phosphorylation may in part account for the increase in phospholipase D activity by Fas cross-linking and D609 can block not only PC-PLC activity but also tyrosine phosphorylation involved in Fas-induced phospholipase D activation.
Animal
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Antibodies, Monoclonal/immunology/*pharmacology
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Antigens, CD95/immunology/*metabolism
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Bridged Compounds/*pharmacology
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Cell Line
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Cross-Linking Reagents
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Dose-Response Relationship, Immunologic
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Enzyme Activation
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Genistein/pharmacology
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Hydrolysis
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Lymphoma/pathology
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Mice
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Phospholipase C/*antagonists & inhibitors
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Phospholipase D/*metabolism
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Phosphorylation
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Phosphorylcholine/metabolism
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Solubility
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Thiones/*pharmacology
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Tumor Cells, Cultured
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Tyrosine/*metabolism
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Water/chemistry
10.Fas-mediated apoptosis and expression of related genes in human malignant hematopoietic cells.
Kyung Mi KIM ; Kee Hyun LEE ; Young Sook HONG ; Hae Young PARK
Experimental & Molecular Medicine 2000;32(4):246-254
Fas transduces apoptotic signals upon cross-linking with the Fas ligand (FasL), which is experimentally replaced by agonistic anti-Fas monoclonal antibodies (mAb). Of eight human malignant hematopoietic cell lines (HL-60, KG-1, THP-1, K562, U937, Jurkat, IM-9, RPMI-8226) examined by flow cytometric analysis, all, except K562, were found to be positive for surface Fas antigen. However, despite surface Fas expression, the agonistic anti-Fas mAb (7C11) induced apoptosis in only three of seven Fas-expressing cell lines (KG-1, Jurkat and IM-9). This Fas-resistance did not correlated with high levels of mRNA either for DcR3, a decoy receptor for FasL, or for FAP-1, a Fas-associated phosphatase that can block the apoptotic function of Fas. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis did not show consistent differences in the expression of Bcl-2 and Bax between Fas-sensitive and Fas-resistant cell lines examined. These findings indicated that the presence or absence of mRNA expression of DcR3, FAP-1, Bcl-2 and Bax did not always correlate with relative sensitivity to Fas-mediated apoptosis. Treatment of cells with cycloheximide converted the phenotype of resistant cell lines from Fas-resistant to Fas-sensitive, and enhanced the sensitivity of Fas-sensitive cell lines. These results suggest that the Fas-resistance is dependent on the presence of labile proteins that determine resistance to Fas-mediated apoptosis and the apoptotic machinery is already in place in Fas-resistant cell lines.
Antigens, CD95/*metabolism
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Apoptosis/drug effects/*genetics
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Carrier Proteins/biosynthesis/genetics
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Comparative Study
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Cycloheximide/pharmacology
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Gene Expression Regulation, Neoplastic
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Hematologic Neoplasms/*genetics/metabolism
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Human
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Membrane Glycoproteins/*metabolism
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Protein Synthesis Inhibitors/pharmacology
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Protein-Tyrosine-Phosphatase/biosynthesis/genetics
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Proto-Oncogene Proteins/biosynthesis/genetics
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Proto-Oncogene Proteins c-bcl-2/biosynthesis/genetics
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Receptors, Cell Surface/biosynthesis/genetics
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Signal Transduction
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Support, Non-U.S. Gov't
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Tumor Cells, Cultured