1.Expression of CD55 and CD59 in chronic rhinosinusitis and its significance.
Lei XIA ; Yinfeng WANG ; Wei GAO ; Hong GAO
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2014;49(12):1021-1023
OBJECTIVETo understand the expression of complement regulatory proteins CD55 and CD59, which secreted by epithelial cells of normal and chronic sinusitis patients.
METHODSCell culture and flow cytometry were used to detect the expression of complement regulatory proteins CD55 and CD59.SPSS 18.0 software was used to analyze the data.
RESULTSCD55 was expressed both in normal nasal mucosa and mucosa of chronic sinusitis. The expression in normal group was 0.001 ± 0.001, significantly lower than that in CRS group which was 0.067 ± 0.028 (t = -10.535, P < 0.01). CD59 was also expressed in the two groups . In normal group, the expression of CD59 was 0.879 ± 0.005, significantly higher than that in CRS group which was 0.238 ± 0.034 (t = 83.416, P < 0.01).
CONCLUSIONSThe nasal mucosa in CRS patients showed low expression of CD55 and high expression of CD59. This mechanism may be involved in the occurrence of CRS.
CD55 Antigens ; metabolism ; CD59 Antigens ; metabolism ; Female ; Flow Cytometry ; Humans ; Rhinitis ; metabolism ; Sinusitis ; metabolism
2.Effects of exposure to simulate unpressurised and dry state of disable submarine on the distributions of CD55 and CD59 on blood cellular membrane.
Rui-yong CHEN ; Miao MENG ; Jun ZHANG
Chinese Journal of Applied Physiology 2007;23(2):226-228
Biomimetics
;
Blood Cells
;
metabolism
;
CD55 Antigens
;
blood
;
CD59 Antigens
;
blood
;
Cell Membrane
;
Humans
;
Male
;
Submarine Medicine
4.Beyond the Role of CD55 as a Complement Component
So Hee DHO ; Jae Cheong LIM ; Lark Kyun KIM
Immune Network 2018;18(1):e11-
The complement is a part of the immune system that plays several roles in removing pathogens. Despite the importance of the complement system, the exact role of each component has been overlooked because the complement system was thought to be a nonspecific humoral immune mechanism that worked against pathogens. Decay-accelerating factor (DAF or CD55) is a known inhibitor of the complement system and has recently attracted substantial attention due to its role in various diseases, such as cancer, protein-losing enteropathy, and malaria. Some protein-losing enteropathy cases are caused by CD55 deficiency, which leads to complement hyperactivation, malabsorption, and angiopathic thrombosis. In addition, CD55 has been reported to be an essential host receptor for infection by the malaria parasite. Moreover, CD55 is a ligand of the seven-span transmembrane receptor CD97. Since CD55 is present in various cells, the functional role of CD55 has been expanded by showing that CD55 is associated with a variety of diseases, including cancer, malaria, protein-losing enteropathy, paroxysmal nocturnal hemoglobinuria, and autoimmune diseases. This review summarizes the current understanding of CD55 and the role of CD55 in these diseases. It also provides insight into the development of novel drugs for the diagnosis and treatment of diseases associated with CD55.
Antigens, CD55
;
Autoimmune Diseases
;
Complement System Proteins
;
Diagnosis
;
Hemoglobinuria, Paroxysmal
;
Immune System
;
Immunotherapy
;
Malaria
;
Parasites
;
Protein-Losing Enteropathies
;
Thrombosis
5.A Novel Marker for Screening Paroxysmal Nocturnal Hemoglobinuria Using Routine Complete Blood Count and Cell Population Data.
Jimin KAHNG ; Yonggoo KIM ; Jung Ok KIM ; Kwangsang KOH ; Jong Wook LEE ; Kyungja HAN
Annals of Laboratory Medicine 2015;35(1):35-40
BACKGROUND: Final diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) may take years demanding a quick diagnosis measure. We used the facts that PNH cells are damaged in acid, and reagents for measuring reticulocytes in Coulter DxH800 (Beckman Coulter, USA) are weakly acidic and hypotonic, to create a new PNH screening marker. METHODS: We analyzed 979 complete blood counts (CBC) data from 963 patients including 57 data from 44 PNH patients. Standard criteria for PNH assay for population selection were followed: flow cytometry for CD55 and CD59 on red blood cells (RBCs) to a detection level of 1%; and fluorescent aerolysin, CD24 and CD15 in granulocytes to 0.1%. Twenty-four PNH minor clone-positive samples (minor-PNH+) were taken, in which the clone population was <5% of RBCs and/or granulocytes. Excluding PNH and minor-PNH+ patients, the population was divided into anemia, malignancy, infection, and normal groups. Parameters exhibiting a distinct demarcation between PNH and non-PNH groups were identified, and each parameter cutoff value was sought that includes the maximum [minimum] number of PNH [non-PNH] patients. RESULTS: Cutoff values for 5 selected CBC parameters (MRV, RDWR, MSCV, MN-AL2-NRET, and IRF) were determined. Positive rates were: PNH (86.0%), minor-PNH+ (33.3%), others (5.0%), anemia (13.4%), malignancy (5.3%), infection (3.7%), normal (0.0%); within anemia group, aplastic anemia (40.0%), immune hemolytic anemia (11.1%), iron deficiency anemia (1.6%). Sensitivity (86.0%), specificity (95.0%), PPV (52.1%), and NPV (99.1%) were achieved in PNH screening. CONCLUSION: A new PNH screening marker is proposed with 95% specificity and 86% sensitivity. The flag identifies PNH patients, reducing time to final diagnosis by flow cytometry.
Antigens, CD15/metabolism
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Antigens, CD24/metabolism
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Antigens, CD55/metabolism
;
Antigens, CD59/metabolism
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Biomarkers/metabolism
;
Blood Cell Count
;
Erythrocytes/cytology/metabolism
;
Flow Cytometry
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Granulocytes/cytology/metabolism
;
Hemoglobinuria, Paroxysmal/*diagnosis/metabolism
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Humans
;
Sensitivity and Specificity
6.The Stromal Overexpression of Decay Accelerating Factor (DAF/CD55) Correlates with Poor Clinical Outcome in Colorectal Cancer Patients.
Tae Hwa BAEK ; Joo Heon KIM ; Mee Ja PARK ; Hye Kyung LEE ; Hyun Jin SON ; Hyun Ki SOON ; Chang Nam KIM ; Che Myong KO ; Dong Wook KANG
Korean Journal of Pathology 2011;45(5):445-454
BACKGROUND: Decay accelerating factor (DAF/CD55), regulates the complement system by accelerating decay of the C3 convertase, has been described in several malignancies, however, the clinicopathologic significance of CD55 and its receptor CD97 has not been fully investigated. We examined the expression patterns of both CD55 and CD97 and their association with clinicopathologic parameters in colorectal cancers (CRCs). METHODS: Expression patterns of CD55 and CD97 in the stroma and tumor cells at tumor center and invasive front were examined in 130 CRCs, and their significance was statistically evaluated. RESULTS: CD55-high stroma was correlated with tumor border (p=0.006) and invasion depth (p=0.013). CD55-high tumor cells at tumor center and invasive front were correlated with histologic grade, and CD55-high tumor cells at invasive front with tumor, node and metastasis (TNM) stage (p<0.05). CD97-high stroma was correlated with lymph node metastasis (p=0.016) and TNM stage (p=0.030). CD97-high tumor cells at tumor center and invasive front were correlated with tumor size and CD97-high tumor cells at tumor center with tumor border (p<0.05). Patients with CD55-high stroma showed poor overall and recurrence-free survival (p<0.05) in univariate analysis, and were independently associated with short recurrence-free survival (p=0.025) in multivariate analysis. CONCLUSIONS: Stromal CD55 overexpression would be an indicator of adverse clinical outcome and a useful prognostic factor.
Antigens, CD55
;
Calcium Hydroxide
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Colorectal Neoplasms
;
Complement C3-C5 Convertases
;
Complement System Proteins
;
Humans
;
Immunohistochemistry
;
Lymph Nodes
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Neoplasm Metastasis
;
Zinc Oxide
7.Expression of Complement Regulator Genes in Abeta1-42 Stimulated Human Neuroblastoma Cell.
Young Sook CHOI ; Kwang Soo LEE ; Sang Ho KIM
Journal of the Korean Neurological Association 2003;21(5):513-520
BACKGROUND: Endogenous complement inhibitors in the brain may protect against the neuroinflammation in Alzheimer's disease. Human neuroblastoma cells were stimulated by Abeta1 - 4 2 to investigate whether the expression of various complement regulator genes is upregulated. METHODS: SK-N-SH cells were incubated overnight with a single dose of 20 microM of Abeta1-42 or 0.5 ng/ml - 5 ng/ml of TNFalpha or both. Actinomycin D (2.5 microM) or cycloheximide (2.5 microM) was also added to the cell suspension. Messenger RNA expression of decay accelerating factor (DAF), membrane cofactor protein (MCP), CD59, complement-receptor 1(CR1), C1 inhibitor (C1-INH), C4-binding protein, factor H, factor I, clusterin and S-protein was measured by RT-PCR. RESULTS: Abeta1-42 and TNFalpha upregulated the expression of C1- INH significantly but increased expression of mRNA for factor H was not statistically significant. The expression of mRNAs for DAF and MCP was at low a level after stimulation. Factor I, CD59 and clusterin were not changed in their mRNA level. The mRNAs for S-protein, C4-binding protein and CR1 were not detected. Actinomycin D suppressed mRNA levels of C1-INH and CD59 significantly. Cycloheximide also inhibited the expression of both C1-INH and CD59. CONCLUSIONS: Early upregulated expression of C1-INH in Abeta1-42 stimulated neuroblastoma cell may contribute to a host defense mechanism against complement-mediated neuronal cell damage.
Alzheimer Disease
;
Amyloid beta-Peptides
;
Antigens, CD46
;
Antigens, CD55
;
Antigens, CD59
;
Brain
;
Clusterin
;
Complement Factor H
;
Complement System Proteins*
;
Cycloheximide
;
Dactinomycin
;
Fibrinogen
;
Genes, Regulator*
;
Humans*
;
Neuroblastoma*
;
Neurons
;
RNA, Messenger
;
Tumor Necrosis Factor-alpha
8.Expression of Complement Regulator Genes in Abeta1-42 Stimulated Human Neuroblastoma Cell.
Young Sook CHOI ; Kwang Soo LEE ; Sang Ho KIM
Journal of the Korean Neurological Association 2003;21(5):513-520
BACKGROUND: Endogenous complement inhibitors in the brain may protect against the neuroinflammation in Alzheimer's disease. Human neuroblastoma cells were stimulated by Abeta1 - 4 2 to investigate whether the expression of various complement regulator genes is upregulated. METHODS: SK-N-SH cells were incubated overnight with a single dose of 20 microM of Abeta1-42 or 0.5 ng/ml - 5 ng/ml of TNFalpha or both. Actinomycin D (2.5 microM) or cycloheximide (2.5 microM) was also added to the cell suspension. Messenger RNA expression of decay accelerating factor (DAF), membrane cofactor protein (MCP), CD59, complement-receptor 1(CR1), C1 inhibitor (C1-INH), C4-binding protein, factor H, factor I, clusterin and S-protein was measured by RT-PCR. RESULTS: Abeta1-42 and TNFalpha upregulated the expression of C1- INH significantly but increased expression of mRNA for factor H was not statistically significant. The expression of mRNAs for DAF and MCP was at low a level after stimulation. Factor I, CD59 and clusterin were not changed in their mRNA level. The mRNAs for S-protein, C4-binding protein and CR1 were not detected. Actinomycin D suppressed mRNA levels of C1-INH and CD59 significantly. Cycloheximide also inhibited the expression of both C1-INH and CD59. CONCLUSIONS: Early upregulated expression of C1-INH in Abeta1-42 stimulated neuroblastoma cell may contribute to a host defense mechanism against complement-mediated neuronal cell damage.
Alzheimer Disease
;
Amyloid beta-Peptides
;
Antigens, CD46
;
Antigens, CD55
;
Antigens, CD59
;
Brain
;
Clusterin
;
Complement Factor H
;
Complement System Proteins*
;
Cycloheximide
;
Dactinomycin
;
Fibrinogen
;
Genes, Regulator*
;
Humans*
;
Neuroblastoma*
;
Neurons
;
RNA, Messenger
;
Tumor Necrosis Factor-alpha
9.Evaluation of allogenic complement-regulating proteins in the peripheral blood cells of chimeric rat models harboring human cord blood stem cells.
Guo-an LIN ; Yi-ping ZHOU ; Xiao-dong YANG ; Jin WEN
Journal of Southern Medical University 2008;28(9):1586-1590
OBJECTIVETo analyze the mechanisms of surrogate tolerogenesis induced by chimeric donors.
METHODSHematopoietic stem cells (HSCs) from human cord blood were transplanted into fetal rats via intrauterine injection and infused into the liver of the neonatal rats to establish chimeric rat models with human HSCs. Four weeks after birth, flow cytometry was performed to analyze the percentages of human CD45 (hCD45), CD55 (hCD55) and CD59 (hCD59)-positive cells in the peripheral blood cells of the chimeric rats. The distributions of hCD55- and hCD59-positive cells in different hCD45/SSC gating regions were observed. The resistance of the peripheral blood lymphocytes to complements-mediated cytolysis was assessed by complement-dependent cytotoxicity (CDC) test in the chimeric rats and compared with that in control rats. The correlation between CDC and the human complement-regulating proteins in the chimeric rats were analyzed statistically.
RESULTSOn hCD45/SSC gating, the percentages of hCD55- and hCD59-positive cells in hCD45-positives region were (53.69-/+18.23)% and (31.8-/+27.5)%, accounting for (2.0-/+1.32)% and (0.76-/+0.56)% of the total cell population, respectively, which were significantly lower than the cell percentages in the extensive region (t=2.71, P=0.043 and t=3.64, P=0.015). The cytolytic rate of PBLs incubated with normal human serum was (22.32-/+15.10)% in the chimeric rats, significantly lower than that in the non-chimeric rats [(60.7-/+22.65)%, t=4.16, P<0.001). In the chimeric rats, hCD55-positive cell percentage was inversely correlated in the peripheral blood karyocytes the cytolysis rate in CDC (r=-0.679, P=0.031), and positively correlated to hCD45-positive cell percentage (r=0.658, P=0.038).
CONCLUSIONThe hCD45-positives region is the cluster of chimeric human cells expressing human complement-regulating proteins. The peripheral blood lymphocytes from chimeric donor can resist the cytolysis mediated by human complement. The presence of allogenic CD55 and CD59 antigens in chimeric donors may be the basis of surrogate tolerogenesis for xenotransplantation.
Animals ; CD55 Antigens ; blood ; CD59 Antigens ; blood ; Complement System Proteins ; analysis ; Cord Blood Stem Cell Transplantation ; methods ; Female ; Humans ; Leukocyte Common Antigens ; blood ; Male ; Models, Animal ; Pregnancy ; Rats ; Rats, Sprague-Dawley ; Transplantation Chimera ; blood ; genetics ; immunology ; Transplantation Tolerance ; Transplantation, Heterologous
10.Expression of three kinds of GPI-anchor proteins in paroxysmal nocturnal hemoglobinuria, aplastic anemia and myelodysplastic syndromes patients and their clinical implications.
Xiao-yuan DONG ; Cong-gao XU ; Guo-rui SUN ; Ti ZHANG ; Jun PENG
Chinese Journal of Hematology 2004;25(4):198-201
OBJECTIVETo investigate the expressions of three kinds of glycosyl-phosphatidylinositol anchor proteins (GPI-AP), the CD(55), CD(59) and CD(87) on the peripheral granulocytes and the soluble u-PAR (su-PAR) level in serum from patients with paroxysmal nocturnal hemoglobinuria (PNH), aplastic anemia (AA), and myelodysplastic syndromes (MDS), and to analyse their clinical implications.
METHODSTwenty-two PNH patients, including 4 complicated with thrombotic diseases and 5 with AA-PNH syndrome, 30 AA patients, including 9 being severe AA (SAA) and 11 chronic AA (CAA), 27 MDS-RA patients, and 20 healthy individuals were tested. The expressions of CD(55), CD(59) and CD(87) on peripheral granulocytes were analyzed with flow cytometry. Serum su-PAR was assayed by ELISA.
RESULTSThe CD(55)(+), CD(59)(+) and CD(87)(+) granulocytes in peripheral blood of 20 normal controls were all more than 90%. The expressions of three kinds of GPI-APs in 22 PNH patients were significantly decreased as compared with that in normal controls, AA patients and MDS-RA patients. The serum level of su-PAR in PNH group was higher than that of the other three groups. The expression of CD(87) was significantly decreased in thrombotic PNH patients as compared with that in non-thrombotic PNH patients. The expression of CD(87) was significantly decreased in AA patients than in normal controls. The expressions of three kinds of GPI-APs in 5 AA-PNH syndrome patients were remarkably reduced as compared with AA patients, but no significant difference was found for these indexes between AA-PNH syndrome and PNH patients and between 27 MDS-RA patients and 20 normal controls.
CONCLUSIONMeasurement of CD(55), CD(59) and CD(87) expressions levels on the peripheral granulocytes and su-PAR in serum would be alternative approaches for diagnosis and differential diagnosis of PNH. Serum level of su-PAR may be helpful to monitor thrombosis in PNH patients.
Adult ; Aged ; Anemia, Aplastic ; blood ; CD55 Antigens ; blood ; CD59 Antigens ; blood ; Female ; Glycosylphosphatidylinositols ; blood ; Hemoglobinuria, Paroxysmal ; blood ; Humans ; Male ; Middle Aged ; Myelodysplastic Syndromes ; blood ; Receptors, Cell Surface ; blood ; Receptors, Urokinase Plasminogen Activator