1.Pro-inflammatory Cytokine Expression of Spleen Dendritic Cells in Mouse Toxoplasmosis.
Ho Woo NAM ; Hye Jin AHN ; Hyun Jong YANG
The Korean Journal of Parasitology 2011;49(2):109-114
Dendritic cells have been known as a member of strong innate immune cells against infectious organelles. In this study, we evaluated the cytokine expression of splenic dendritic cells in chronic mouse toxoplasmosis by tissue cyst-forming Me49 strain and demonstrated the distribution of lymphoid dendritic cells by fluorescence-activated cell sorter (FACS). Pro-inflammatory cytokines, such as IL-1alpha, IL-1beta, IL-6, and IL-10 increased rapidly at week 1 post-infection (PI) and peaked at week 3 PI. Serum IL-10 level followed the similar patterns. FACS analysis showed that the number of CD8alpha+/CD11c+ splenic dendritic cells increased at week 1 and peaked at week 3 PI. In conclusion, mouse splenic dendritic cells showed early and rapid cytokine changes and may have important protective roles in early phases of murine toxoplasmosis.
Animals
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Antigens, CD11c/analysis
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Antigens, CD8/analysis
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Cytokines/*blood/*secretion
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Dendritic Cells/chemistry/*immunology
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Disease Models, Animal
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Flow Cytometry
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Mice
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Mice, Inbred BALB C
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Rodent Diseases/immunology
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Spleen/*immunology
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Time Factors
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Toxoplasmosis, Animal/*immunology
2.Surface markers and functions of human dendritic cells exposed to mobile phone 1800 MHz electromagnetic fields.
Zhi-dong ZHOU ; Qun-li ZENG ; Yun ZHENG ; Jian-bin ZHANG ; Hai-yang CHEN ; De-qiang LU ; Chuan-sen SHAO ; Da-jing XIA
Journal of Zhejiang University. Medical sciences 2008;37(1):29-33
OBJECTIVETo investigate the effects of mobile phone 1800 MHz electromagnetic fields (EMF) on the surface markers and the functions of human dendritic cells (DC).
METHODSHuman DCs were exposed to intermittent 5 min on/10 min off EMF with specific absorption rates (SAR) 4 W/kg for 0 h, 1 h, 12 h or 24 h, respectively. FACS analysis was used to detect the positive percentage of DC surface markers including HLA-DR and co-stimulatory molecules such as CD80, CD86, CD40 and CD11c. CCK-8 kit was adopted to examine the function of allo-mixed lymphocyte reaction (allo-MLR) of DC, and enzyme linked immunosorbent assay (ELISA) to identify the levels of IL-12p70 and TNF-alpha secreted by DC.
RESULTCompared with the sham radiation group, after exposure to the electromagnetic fields for 1 h, 12 h, or 24 h, HLA-DR, CD80,CD86 and CD40 were all declined except CD11c. The ability of DC allo-MLR in each exposure group was decreased significantly (P<0.05), especially in the 24 h exposure group. However, the secreted levels of IL-12p70 and TNF-alpha of DC in each exposure group remained no changed.
CONCLUSIONThe study showed that EMF exposure could down-regulate the surface molecules and stimulation ability of human DC.
B7-1 Antigen ; B7-2 Antigen ; immunology ; Biomarkers ; analysis ; CD11c Antigen ; immunology ; Cell Phone ; Cells, Cultured ; Dendrites ; pathology ; Dendritic Cells ; metabolism ; physiology ; radiation effects ; Electromagnetic Fields ; HLA-DR Antigens ; analysis ; Humans ; Interleukin-12 ; immunology