CTK is one kind of traditional medicine drug for RTIs treatment. The toxicity of CTK was studied and the result of clinical trial has proved its effectiveness in RTI treatment. Objectives: To investigate the antibiotic effects of C.T.K. on some kinds of bacteria caused reproductive tract infections (RTIs). Methods: This experimental study was performed on the group of standardized bacteria and the bacteria isolated from patients with RTIs. The results showed that: C.T.K inhibited absolutely the growth of the standardized S. aureus ATCC 25923 and standardized E.coli ATCC 25922 at the level of CTK diluted strength 1/150 and with at the level of 1/100. With the bacteria isolated from RTI patients: At the level of C.T.K diluted strength 1/50, CTK could inhibit 100% the growth of S. aureus and 90% the growth of E.coli. C.T.K could not inhibit the growth of both bacteria at the level of C.T.K diluted strength 1/150. Conclusion: The results proved that C.T.K has antibiotic effect on S. aureus and E.coli at different levels of drug diluted strength.
Anti-Bacterial Agents, Bacteria, Medicine, Traditional

Althaea rosea is a type of mallow plant. Its dry flowers are one of common herb in Uyghur medicines and recorded to have several efficacies such as external application for detumescence, moistening lung and arresting cough, sweating and relieving asthma, diminishing swelling and promoting eruption, soothing the nerves and strengthening heart. However, there are only fewer studies on effective components of A. rosea and no literature about its volatile oil and pharmacological activity. In this paper, the volatile oil of A. rosea was obtained by using the chemical distillation and extraction method. The individual chemical components were separated from the volatile oil and identified by the Gas Chromatograph-Mass Spectrometer technique (GC-MS). The antioxidant activity against free radicals was detected by the'ultraviolet and visible spectrophotometer method. The antibiotic activity was detected by the filter paper diffusion method. The experimental results showed nearly 70 compounds in the volatile oil, with complex chemical components. With a low content, most of the compounds were aromatic and aliphatic compounds and their derivatives. A. rosea had a better antibiotic activity for common microorganisms, with a wide antibacterial spectrum. According to the results, the volatile oil of A. rosea will have a good application value in medicine, food and cosmetic industries, which provided a scientific basis for the development of natural A. rosea resources.
Althaea ; chemistry ; Anti-Bacterial Agents ; chemistry ; pharmacology ; Antioxidants ; chemistry ; pharmacology ; Bacteria ; drug effects ; China ; ethnology ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Gas Chromatography-Mass Spectrometry ; Medicine, Chinese Traditional ; Oils, Volatile ; chemistry ; pharmacology ; Plant Oils ; chemistry ; pharmacology

OBJECTIVETo evaluate the in vitro antibacterial properties and the ability to potentiate some common antibiotics effects of the methanol extracts of 11 Cameroonian food plants on 29 Gram-negative bacteria expressing multidrug resistant (MDR) phenotypes.

METHODSThe antimicrobial activity of the extracts was performed using the broth microdilution method. The phytochemical screening of these extracts was also performed using standard methods.

RESULTSOcimum basilicum, Gnetum africanum and Eucalyptus robusta extracts possessed an antibacterial activity against all the 29 studied bacteria. The extracts from G. africanum and E. robusta were the most active with the lowest minimal inhibitory concentration of 64 μg/mL on Escherichia coli AG100A for both extracts and also against Klebsiella pneumoniae K24 for G. africanum. When tested in the presence of phenylalanine-arginine β-Naphtylamide (PAβN), an efflux pump inhibitor, the extract of Thymus vulgaris and E. Robusta showed the best activities on most tested strains. E. Robusta extract showed good synergistic effects, improving the activity of commonly used antibiotics in about 85% of cases.

CONCLUSIONThe overall results obtained provide the baseline information for the use of the tested plants in the treatment of bacterial infections.

Anti-Bacterial Agents ; pharmacology ; Cameroon ; Drug Resistance, Multiple ; drug effects ; Eucalyptus ; chemistry ; Gnetum ; chemistry ; Gram-Negative Bacteria ; drug effects ; Gram-Negative Bacterial Infections ; drug therapy ; microbiology ; Humans ; Medicine, African Traditional ; methods ; Microbial Sensitivity Tests ; Ocimum basilicum ; chemistry ; Phytotherapy ; methods ; Plant Preparations ; pharmacology ; Plants, Edible ; chemistry

OBJECTIVEMyanmar has a long history of using medicinal plants for treatment of various diseases. To the best of our knowledge there are no previous reports on antiglycation activities of medicinal plants from Myanmar. Therefore, this study was aimed to evaluate the antioxidant, antiglycation and antimicrobial properties of 20 ethanolic extracts from 17 medicinal plants indigenous to Myanmar.

METHODSIn vitro scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), superoxide (SO) radicals were used to determine the antioxidant activities. Folin-Ciocalteu's method was performed to determine the total phenolic content. Antiglycation and antimicrobial activities were detected by bovine serum albumin-fluorescent assay and agar well diffusion method.

RESULTSTerminalia chebula Retz. (Fruit), containing the highest total phenolic content, showed high antioxidant activities with inhibition of 77.98% ± 0.92%, 88.95% ± 2.42%, 88.56% ± 1.87% and 70.74%± 2.57% for DPPH, NO, SO assays and antiglycation activity respectively. It also showed the antimicrobial activities against Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans with inhibition zone of 19, 18, 17, 25 and 15 mm, respectively. Garcinia mangostana Linn. showed the strongest activities for SO and antiglycation assays with inhibition of 93.68% ± 2.63% and 82.37% ± 1.78%. Bark of Melia sp. was the best NO radical scavenger with inhibition rate of 89.39%± 0.60%.

CONCLUSIONThe results suggest that these plants are potential sources of antioxidants with free radical-scavenging and antiglycation activities and could be useful for decreasing the oxidative stress and glycation end-product formation in glycation-related diseases.

Anti-Bacterial Agents ; analysis ; pharmacology ; Anti-Infective Agents ; analysis ; pharmacology ; Antioxidants ; analysis ; pharmacology ; Bacteria ; drug effects ; growth & development ; Biphenyl Compounds ; metabolism ; Candida albicans ; drug effects ; growth & development ; Fruit ; Garcinia ; chemistry ; Glycation End Products, Advanced ; metabolism ; Humans ; Magnoliopsida ; chemistry ; Medicine, Traditional ; Melia ; chemistry ; Myanmar ; Nitric Oxide ; metabolism ; Oxidative Stress ; drug effects ; Phenols ; analysis ; pharmacology ; Phytotherapy ; Picrates ; metabolism ; Plant Bark ; Plant Extracts ; chemistry ; pharmacology ; Plants, Medicinal ; Superoxides ; Terminalia ; chemistry