OBJECTIVEThe purpose of this study was to investigate the regulatory role of tyrosine kinase 2 with immunoglobulin-like and epidermal growth factor homology domains (Tie2) on apoptosis and proliferation in the endothelial cells.

METHODSRNA interference (RNAi) technique was used to silence Tie2 gene expression by transfecting an expression vector containing short hairpin RNA(shRNA) for Tie2 into human umbilical vein endothelial cells (HUVECs). Real time quantitation reverse transcriptase polymerase chain reaction (QRT-PCR) and Western blot were used to monitor Tie2 mRNA, as well as protein expression. The proliferation of HUVECs was examined by methyl thiazolyl tetrazolium (MTT), and the apoptosis was detected under microscope. HUVECs transfected with pGenesil-hk was negative control, and HUVECs transfected with nothing was empty control.

RESULTSTie2 mRNA expression was down-regulated 24 h and 48 h after transfection, and Tie2 protein expression was significantly down-regulated at 24 h and 48 h (P< 0.05), especially 48 h after transfection. The apoptosis rate was conspicuously higher in experimental group than in negative control and empty control group after 48 h (P<0.05). The growth monitoring showed that proliferation was also markedly inhibited in experimental group (P<0.05) compared with two control groups.

CONCLUSIONDown-regulated expression of Tie2 by RNAi can promotes apoptosis of HUVECs and has an anti-proliferation activity effect on them.

Apoptosis ; Cell Line, Tumor ; Cell Proliferation ; Down-Regulation ; EGF Family of Proteins ; Human Umbilical Vein Endothelial Cells ; Humans ; Immunoglobulins ; RNA Interference ; RNA, Messenger ; RNA, Small Interfering ; TYK2 Kinase ; Transfection

Objective: To explore the application and effect of the PDCA cycle nursing management model in the treatment of peri-implant mucositis. Methods : Thirty patients with peri-implant mucositis were treated nonsurgically. Before treatment, the 30 patients had no history of systemic diseases, drug allergies, or bad habits. According to the principle of single-blind randomized control, the patients were divided into two groups: 15 patients were assigned to the control group and received routine clinical nursing and oral hygiene education according to the doctor′s prescription; and 15 patients were assigned to the intervention group, in which the PDCA cycle nursing management model was adopted. The four steps of “plan, do, check and act” were carried out. The plaque index (PL), gingival index (GI) and probe depth (PD) in the two groups were recorded before treatment and 3 and 6 months after treatment. Results: There was no significant difference in the PL, GI or PD between the intervention group and the control group before treatment (P > 0.05). Three months after treatment, the PL in the intervention group was 1.25 ± 0.44, while the PL in the control group was 1.49 ± 0.39, with a significant difference (t=2.56, P=0.008); the GI in the intervention group was 1.21 ± 0.43, while the GI in the control group was 1.56 ± 0.37, with significant difference (t=2.94, P=0.006); and the PD in the intervention group was 4.39 ± 0.41 while the PD in the control group was 4.47 ± 0.52 mm, with no significant difference (t=2.24, P=0.062). Six months after treatment, the PL in the intervention group was 1.26 ± 0.48, while the PL in the control group was 1.51 ± 0.42, with a significant difference (t=2.66, P=0.007); the GI in the intervention group was 1.34 ± 0.28, while the GI in the control group was 1.74 ± 0.48 (t=2.98, P=0.008); and the PD in the intervention group was 4.46 ± 0.52 mm, while the PD in the control group was 4.54 ± 0.66, with no significant difference (t=2.28, P=0.077). Conclusion The PDCA cycle nursing management model can enhance patients′ awareness of oral health maintenance, reduce gingival plaque accumulation, and effectively improve the health status of peri-implant tissues.