1.Human optic nerve head astrocytes culture in vitro:Ⅰ. the primary culture and passage
Wei-Jia, DAI ; Culp-Stewwart SHELLEY ; Cheng, ANNA ; Flanagan JOHN ; Ethier Ross C
International Eye Science 2008;8(7):1311-1314
AIM: To culture astrocytes from human donor eyes in order to understand the function of astrocytes in remodelling events in the glaucomatous optic nerve head (ONH).METHODS: Primary cultures were prepared by explantation of human ONH tissue in order to get astrocytes. Laminar criborsa (LC) cells were prepared concurrently for comparison. Astrocyte cultures could be separated from LC cells by selecting medium.Similar procedures were used for LC.RESULTS: Primary ceils grew from human optic nerve head explants 4-8 weeks after explantation. Astrocytes had different morphologies and growth characteristics from LC cells. Type 1B astrocyte cells could grow in medium without FBS. Purified cultures were obtained by second passage and could be harvested by third to fifth passage, which were prepared to use for further study, including being characterized by positive glial fibrillary acidic protein (GFAP) and neural cell adhesion molecule (NCAM) staining.CONCLUSION: Precise dissection of fragment is the most important step to get clear explants for primary culture. Economic and rapid method could be useful to select cells by different mediums, which will help us to get more purified cells for further study.
2. Long-term stability of periodontal root coverage procedures for gingival recession
Anna DAI ; Peihui DING ; Qi TANG ; Mengxin YANG ; Lili CHEN
Chinese Journal of Stomatology 2019;54(2):124-129
Gingival recession could result in root exposure, dental hypersensitivity and poor aesthetics. It has been demonstrated that varieties of root coverage procedures can significantly improve gingival recession in short-term (≤6 months), of which coronally advanced flap combined with connective tissue graft is the gold standard technique for treatment of gingival recession. It could obtain the optimally complete root coverage and maintain long-term stability (≥2 years). However, clinical knowledge about the long-term effectiveness of the other alternative graft materials remain very limited. Based on the existing clinical evidence, this article reviews coronally advanced flap, coronally advanced flap combined with connective tissue graft or alternative graft materials, with particular attention to the long-term stability of them, in order to provide reference for the design of further clinical trials and the plan of clinical treatments.
3. Blocking effects of serum antibodies against HBGA binding to norovirus GII.4 of population in oyster culture area of Guangdong Province
Yali ZHUANG ; Lin QIN ; Yingchun DAI ; Anna WANG ; Xianwu ZHONG ; Rongfeng CHEN ; Qiong HUANG ; Yonghui ZHANG
Chinese Journal of Preventive Medicine 2018;52(3):287-291
Objective:
Evaluate GII.4 norovirus infection and blocking effects of serum antibodies against HBGAs binding to GII.4 norovirus of population in oyster culture area, provide references for screening of fully human monoclonal antibody.
Methods:
Using a random survey method to collect blood and saliva samples in oyster culture area, select serum samples from the inland region of Guangdong as control group. Identification of salivary HBGA receptor phenotype and detection of serum antibody levels between two areas by ELISA. A vitro neutralization model was to determine the efficiency of serum antibodies blocking GII.4 norovirus and HBGA receptors binding.
Results:
The age were (50.68 ± 15.17), (52.52 ± 15.90) and (51.37 ± 13.32) years old of 2015, 2016 in experimental group, and in control group, respectively. Males accounted for 5.9% (70/195), 36.6%(60/164), 40.8% (69/169) (χ2=0.93,
4.Periodontal therapy for stage Ⅳ grade C generalized periodontitis: A case report of 7-year follow-up
STOMATOLOGY 2023;43(2):145-152
Periodontal disease is individual-specific and site-specific. Therefore, the periodontal sequence treatment plan should not only base on the patient's condition and disease progression, but also take local conditions into account. This paper reports the whole periodontal therapy of a young patient with stage Ⅳ grade C generalized periodontitis with longitude observation of 7 years. We analyze the factors between extraction and maintenance of hopeless teeth from individual-specific and site-specific perspectives. We also discuss the importance of keratinized mucosa around implants in order to provide reference for the treatment of periodontitis.
5.Effect of periodontitis on diabetes: a review of cohort studies
Journal of Prevention and Treatment for Stomatological Diseases 2023;31(10):751-755
Periodontitis is an inflammation that occurs in the supporting tissues around teeth with plaque biofilm as the starting factor. Periodontitis is closely related to many systemic diseases, among which the relationship between periodontitis and diabetes is the most widely reported. A cohort study is an essential clinical research method to explore the etiology. Large, well-conducted prospective cohort studies have high power, which can provide important clinical evidence for the impact of periodontitis on blood sugar control, incidence rate and complications of diabetes mellitus. Periodontitis is associated with the deterioration of glycemic control. At present, there is moderate evidence that nonsurgical periodontal treatment can significantly improve the blood sugar level of diabetes patients with periodontitis compared with no periodontal treatment. Studies on the impact of periodontitis on the incidence rate of diabetes lack consistent conclusions because of different population backgrounds. The evidence regarding whether periodontitis affects the incidence rate of diabetes complications is relatively limited. Therefore, well-designed cohort studies are needed to provide high-quality clinical evidence.