Objective To investigate the effects of morin on chronic obstructive pulmonary disease(COPD)animal and cell model through the regulation of matrix metalloproteinase-9(MMP9).Methods SwissTargetPrediction da-tabase was used to predict the genetargets of morin,GeneCards,OMIMand DisGenet databases were used to predict the risk genes of COPD,the intersection of COPD-related gene targets and morin gene targets was obtained by using Venny online website;Protein-protein interaction(PPI)analysis was performed using the String database,and to-pological analysis was performed by Cytoscape 3.8.2 software.Molecular docking was used to validate the interac-tion between the drug components and gene targets;COPD rat models were constructed to verify the therapeutic effects of morin om COPD rat models.The COPD cell model was established to interfere with the expression of MMP9 in the cells.COPD cell models were established and the expression of MMP9 in cells was intervened;flow cytometry was used to detect cell apoptosis rate;ELISA was used to measure the concentration of inflammatory fac-tors.Results The Swiss Target Prediction database predicted 96 gene targets of morin.GeneCards,OMIM,and DisGenet databases predicted 7 122 gene targets of COPD,intersection analysis identified 67 common gene targets between morin and COPD.Topological analysis revealed that the top 5 gene targets with strongest interactions a-mong the intersected targets were SRC,MMP9,MMP2,PTGS2,and FURIN;Molecular docking results showed the best binding activity between MMP9 and morin;animal experiments showed that morin improved respiratory function parameters and lung tissue pathological damage,and inhibited MMP9 expression in COPD rats(P＜0.05);Cell experiments showed that morin increased cell viability in COPD cell models and inhibited MMP9 ex-pression(P＜0.05).In addition,morin inhibited cell apoptosis and expression of inflammatory factors in the COPD cell model,but this effect was reversed by overexpression of MMP9(P＜0.05).Conclusion Morin can improve apoptosis and and expression of inflammatory cytokines in COPD cell model,reduce lung tissue pathologi-cal changes by inhibiting MMP9 expression.

Objective:To investigate the standardization construction of nursing information terminologies in Guizhou Province and provide reference for constructing a standard nursing terminology system in Guizhou Province.Methods:Using the convenient sampling method, a total of 153 nursing managers from hospitals at all levels in Guizhou Province were selected as the research objects in June, 2021. The research objects were surveyed using a self-developed survey questionnaire on the current status of standardized terminology construction for hospital nursing information. The questionnaire consisted of two parts. The first part was the basic information of the hospital and the general information of the person in charge of nursing information. The second part was the construction of standardized terminology for nursing information, including three dimensions, such as the basic dataset of nursing electronic medical records, the shared document of nursing electronic medical records and the construction of nursing information terminologies interconnection. Multiple linear regression analysis was used to analyze the influencing factors of standardized construction of nursing information terminologies. A total of 153 questionnaires were distributed in this study and 148 valid questionnaires were collected, with an effective response rate of 96.73% (148/153) .Results:The total score of the survey questionnaire on the standardization of nursing information terminologies construction in 148 hospitals in Guizhou Province was (102.22±24.42) . The basic dataset dimension score of nursing electronic medical records was (60.35±13.32) , the shared document dimension score of nursing electronic medical records was (27.78±19.08) and the dimension score of nursing terminology interconnection construction was (14.08±2.71) . The results of multiple linear regression analysis showed that hospital level and whether nursing managers had received terminology training were the influencing factors for the score of hospital nursing information standardization terminology construction ( P<0.05) . Conclusions:The standardization construction of nursing information terminologies in medical institutions in Guizhou Province is still at the primary level of information construction. The insufficient attention paid by secondary hospital managers to the standardization construction of nursing information terminologies and the lack of understanding of standardized nursing terminology by nursing information managers are important influencing factors.