Soft liner materials for denture are applied on the surface of denture base, and present elasticity after solidification. These materials ameliorate the impacted biting force, and relieve or eliminate the tenderness. Moreover, they can gather denture base with alveolar crest closely, and improve dentural retention. Due to the specific physico-chemical properties, soft liner materials play an amortization role between denture base and alveolar crest, and are applicable to the complete denture restoration in patients with serious depauperation of alveolar crest. The properties of soft liner materials vary with different factors, thus affecting the exertion of function. The aim of this paper is to analyze the bonding strength, hydroscopicity and dissolubility, micro-organism attachment, together with aging resistance of soft liner materials, besides, to investigate the influential factors on the physico-chemical properties of soft liner materials for denture, further modify their properties and exert better functions.

This paper reported the history of avian flu outbreaks in China from 1996 to 2006, directed the characteristics of avian influenza outbreaks in recent years. China is one of the biggest poultry producers in the world and many migratory birds go through China, China faces high risk of pandemic bird flu. The continuing occurrence of sporadic human cases indicates that the virus is continuing to circulate in birds in some parts of the country. In the fighting again avian flu virus, China has developed some possible practice on precautionary and control measure. China enhances international cooperation and scientific involvement in the prevention and control of the avian flu. The State Council and Ministry of Agriculture issued the laws and regulations on avian flu. Wild birds may have played a role in getting domestic fowl and human beings infected with bird flu. Poultry manure is considered to be another key source of the spread routes of H5N1 virus, but the bio - security processing in free range poultry farming and waste products is very poor disposition in China. Epidemic control at grassroots level still needs to be strengthened.

Objective To discuss the opportunity of operation and selective in the early stage of treatment method. Methods To analyze the clinical data of 31 cases of patient traumatic floating knee since June 1999 to Au-gust 2006 at my hospital. The results of operative treatment and clinical condition were reviewed. Results In this group there were wound infection 5 cases,amputation 1 case,two stage plant the bone technique and inner fiction 2 cases. After following up patients 1 to 3 years(average 25 months). All of the patients were recovered. According to kerstin at working out standard the limbs function of floating knee damage was evaluated so as to obtain the good percentage of 87 % of the knee joint function. Conclusion A.s usually, the complication of multiple organs injury in floating knee damage was most likely to occur if there had been position priority disposed of important internal organs damage and shock strives in 24 hours properly. The fixed thighbone and shinbone fracture with the emergency dis-posal of important blood vessel damage can reduce the amputation rate.

Objective To investigate the differences in clinical manifestation,laboratory test and imaging between patients with symptomatic and non-symptomatic basilar dolichoectasia (BD).Methods We retrospectively analyzed the patients admitted in our hospital from January 2012 to January 2013,who receiving intracranial computed tomography angiography or magnet resonance angiography.We grouped the patients to symptomatic and non-symptomatic,and further divided the symptoms into vascular events and compressive symptoms.We compared the baseline characteristics of the symptoms in these groups,risk factors of cerebrovascular events,and kidney function between patients with symptomatic and nonsymptomatic BD.Results Comparing with non-symptomatic BD (n--24),the diameter of basilar artery in symptomatic BD patients(n =52) was larger ((4.7 ± 0.9) mm vs (4.2 ± 1.0) mm,t =-2.289,P =0.025),and the level of diastolic blood pressure and uric acid were higher ((94.0 ± 16.7) mmHg vs (86.9--±12.0) mmHg,1 mmHg =0.133 kPa,t =-2.113,P =0.038;(333.3 ± 120.7) μmol/L vs (267.6 ± 84.6) μmol/L,t =-2.724,P =0.008).The group with vascular symptoms (n =18) had greater basilar artery diameter ((4.9 ±0.8) mm vs (4.2 ± 1.0) mm,t =-2.612,P ＜0.05) compared to those without vascular symptoms(n =58).BD patients with compressive symptoms (n =6) had higher level of uric acid ((374.5 ± 51.9) μmol/L vs (281.1 ± 101.6) μmol/L,t =-2.216,P ＜ 0.05) and serum IgA ((1.44 ± 0.28) g/L vs (2.48 ± 0.78) g/L,t =5.660,P ＜ 0.05) than those with non-compressive symptoms (n =70).Conclusions The presence of symptoms in patients with BD is related with the increased diameter of basilar artery.Our results suggest that strict control of blood pressure in the early stage may help to reduce the incidence of the vascular events in BD patients.

Objective By extra-body imitation release test with prolongation,medicines action on sustainedreleased injection of hydro-cortisone microeapsule was understood. Methods Making use of HPLC method was inspected to result of medicinal lower and die, solution. At the same time, the disaolvent course of microcapsule wall was observed by microscope and electron microscope. Results The release test at beginning 12 - 24 hours with rdease drugs measure was very lower-degree,along with the time more prolong in mierocapsule substance to contact of PBS solution(24～48h) was began disintegrate. The rdease of medicinal concentration also accompany with rising, the rising different concentration PBS solution from starting up to the highest as to general need 3～5 days and keep releasing for several days. Conclusion Sustained-released injection of hydrocortisone mierocapsule can be effectual action of prolongation released time in hind hydrocortisone.

Objective To analyze the characteristics and risk factors of preoperative deep vein thrombosis in patients with hip fracture,and provide a theoretical basis for clinical treatment and intervention.Methods 470 patients with hip fracture were chosen as the objects of study.The clinical data of the patients were retrospectively analyzed,including age,gender,injury,combined with other systemic diseases,fracture type (whether old fractures),two D-dimer level.All the patients with deep venous thrombosis of lower extremity were divided into DVT group and nonDVT group.There were 83 cases in DVT group,387 cases in non-DVT group.The factors like aboves as independent variables,presence of deep vein thrombosis as dependent variable,,preoperative deep venous thrombosis of the characteristics and risk factors were analyzed.Results 83 patients had DVT,the incidence rate was 17.7％.There were significant difference in age (40/83 vs 268/387),combined with other system diseases (57/83 vs 283/387),prevention measures (58/83 vs 196/387),D-thrombosis two dimer level (16/83 vs 122/387) of the two groups (x2 =13.712,14.836,9.876,5.313,all P ＜ 0.05).3 age ≥ 40 years,with other system diseases and injury,did not take to prevent the formation of thrombus measures were independent risk factors influencing the occurrence of DVT.Conclusion Age ≥40 years,combined with other system diseases and injury,don't take to prevent the formation of thrombus measures are independent risk factors influencing the occurrence of DVT.

Objective To investigate the potential effects of simvastatin on angiotensin Ⅱ-stimulated secretion and proliferation of adrenocortical carcinoma H295R cells.Methods The H295R cells were divided into control group, Angiotensin Ⅱgroup, simvastatin group and Angiotensin Ⅱ plus simvastatin group.Cortisol in medium was determined by chemiluminescent method , and aldosterone was determined by radioimmunoassay .The mRNA expression of 11 beta-hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) were examined by RT-qPCR.Cell proliferation was detected by MTS method.Results Compared with control group, angiotensin Ⅱincreased the secretion of cortisol and aldosterone, and the expression of CYP11B1 and CYP11B2.Simvastatin decreased cortisol secretion and CYP11B1 mRNA expression ( P<0.05 ) .Simvastatin also inhibited angiotensinⅡ-induced the secretion of cortisol and aldosterone , and the expression of CYP 11 B1 and CYP11 B2 compared with Angiotensin Ⅱgroup ( P<0.05 ) .Angiotensin Ⅱhad no effect on cell proliferation , while simvastatin significantly inhibited cell proliferation .The inhibitory effect of simvastatin on proliferation was enhanced when simvastatin was prescribed with angiotensin Ⅱ( P<0.05 ) .Conclusions Simvastatin inhibits angiotensin Ⅱ-induced secretion of cortisol and aldosterone in H295R cells.Simvastatin inhibits cell proliferation, which could be enhanced by angio-tensin Ⅱ.

Objective:To analyze KCNJ5 mutation of adenomas in patients with aldosterone-producing adenoma (APA) companying with hypokalemia, and to compare the clinical characteristics of patients with and without KCNJ 5 mutations.Methods:Clinical data of 144 APA patients were retrospectively analyzed. DNA were extracted from adenoma tissues, and amplified and sequenced for KCNJ5 gene. The serum potassium level and cardiac complications in patients with and without KCNJ5 gene mutation were compared.Results:Among 144 tumors, 131 tumors (91%) had KCNJ5 mutation, including 68 tumors with G151R, 56 tumors with L168R, 5 tumors with E145Q, and two tumors with novel mutations, V156_K160delITE and G151delinsVR. Compared with patients without KCNJ5 mutation, patients with KCNJ5 mutation had lower preoperative serum potassium levels, more cardiac complications, lower postoperative systolic blood pressure, and better postoperative hypertension relief. There were no statistical differences in age, gender, blood pressure, serum potassium level, plasma renin activity or plasma aldosterone concertration.Conclusion:91% adenomas in patients with APA and hypokalemia had KCNJ5 mutation, suggesting that KCNJ5 mutation is the main cause in these patients.

Objective To explore a simple and effective epicanthoplasty.Methods A total of 65 cases were corrected by incising the epicanthus levelly,extending the incision along the lower palpebral margin,separating the skin about 1 cm×1 cm scope below incision,cutting off part of the orbicularis and epicanthus ligament,fixing epicanthus angle to nasal aponeurosis,and suturing the incision without tension.Results All the cases corrected by the transverse-one's procedure were followup one month to one year,and satisfactory results obtained.Conclusion This method is a simple and effective epicanthoplasty with less tissue damage and good appearance.

ObjectiveTo detect mRNA and protein expression of steroidogenic factor-1 ( SF-1 ) and DAX-1 in human adrenocortical tumors and normal adrenal cortex,and to investigate the effect of SF-1 and DAX-1 on the steroidogenesis and development of adrenocortical tumors.Methods Total RNA and protein was extracted from angiotensin Ⅱ unresponsive aldoterone-producing adenomas ( A Ⅱ -U-APA,n =12 ),angiotensin Ⅱ responsive aldoterone-producing adenomas ( AⅡ -R-APA,n =5 ),cortisol-producing adenomas ( CPA,n =10 ),adrenal nonfunctional adenomas ( NFA,n =10 ),aldosterone-producing carcinoma ( APC,n =2 ) and normal adrenal cortex ( NAC,n =8).To analyze gene expression of SF-1,DAX-1,ACTH receptor(ACTHR),and β-actin by real-time quantitative PCR in different tissues.The protein expression of SF-1,DAX-1,and β-actin in the same tissues by Western blot.To study the relationship of ACTHR,SF-1,and DAX-1 with clinical data in adrenocortical tumors.ResultsThe expression of SF-1,DAX-1 mRNA and protein was different in NAC,AⅡ -U-APA,A Ⅱ -R- APA,APC,CPA,and NFA tissues [ relative expression of SF-1 mRNA:24.58±2.45,23.89±3.17,21.59±3.00,(38.75,44.16),14.17±2.80,and 36.38±3.50; DAX-1 mRNA:0.57±0.06,0.37±0.05,0.43±0.05,( 1.52,1.21 ),0.39 ±0.04,and 0.83 ±0.08 ; SF-1 protein:0.76 ±0.11,0.76 ±0.10,0.73 ±0.07,(1.24,1.40),0.55±0.04,and0.98±0.10; DAX-1 protein:0.65±0.14,0.39±0.13,0.43±0.14,(1.18,1.02),0.56±0.04,and 1.03±0.13 ; all P＜0.05 or P＜0.01 ].There was negative correlation by higher SF-1/DAX-1 ratio and tumor size in AⅡ -U-APA tissues.The mRNA and protein expression of SF-1 was lower in CPA and there was the positive correlation with tumor size.Conclusion SF-1 and DAX-1 might play a key role in the development of the adrenocortical tumorigenesis and steroidogenic tissues.