1.Hemostasis effect comparison between artificial pressing and Perclose vascular closure device on femoral artery
Yongdong LI ; Libin GONG ; Meijun CHEN ; Anjie XU
Chinese Journal of cardiovascular Rehabilitation Medicine 2014;23(5):563-565
Objective:To observe hemostoisis efficacy and safety of Perclose vascular closure device on femoral artery in transfemoral pathway radiofrequency ablation (RFA) of supraventricular tachycardia (SVT) .Methods :A total of 199 patients undergoing transfemoral RFA of SVT were divided into Perclose vascular closure group (Perclose group ,n=98) and artificial pressing group (n=101) .Immediate success rate ,hemostasis time ,braking time and in-cidence rates of postoperative complications were observed between two groups .Results:There was no significant difference in immediate success rate of hemostasis (98% vs .100% ) between Perclose group and artificial pressing group , P> 0.05. Compared with artificial pressing group , there were significant reductions in hemostasis time [ (18.9 ± 9.1) min vs .(2.7 ± 0.7) min] ,braking time [ (21.6 ± 3.4) min vs .(6.3 ± 2.4) min] and total inci-dence rate of complications (16.8% vs .4.1% ) in Perclose group , P<0.05 or <0.01. Complications:incidence rate of vagal reflex in Perclose group was significantly lower than that of artificial pressing group (0 vs .7.9% ,P<0.05) ,other incidence rates of complications between two groups were no significant difference ( P> 0.05 all) . Conclusion:Perclose vascular closure device could shorten the duration of hemostasis and braking ,and reduce the complications .It could be the preferred choice for femoral artery hemostasis .
2.Management of difficult recipient vessel preparation in microsurgical reconstruction for oral and maxillofacial defects with free flaps
Limeng WU ; Canhua JIANG ; Jie CHEN ; Ning LI ; Anjie MIN ; Xing GAO ; Xinchun JIAN
Chinese Journal of Microsurgery 2016;39(2):114-118
Objective To investigate the management of difficult recipient vessel preparation in microsurgical reconstruction for oral and maxillofacial defects with free flaps and to assess the clinical outcome.Methods A total number of 739 patients with oral and maxillofacial defects underwent consecutive free flap reconstruction with 761 free flaps from May,2012 to May,2015.There were 37 patients who could not find or lack of proper recipient vessels for microvascular anastomosis during operation.Among them,22 were recurrent oral cancer after tumor ablation,10 of them undrewent post-operative radiotherapy;5 were second primary oral cancer,7 diagnosed with osteoradionecrosis,and 3 suffered from oral and maxillofacial defect and deformity caused by trauma or inflammation.Forty free flaps including 23 anterolateral thigh flaps (ALT),10 fibular flaps and 7 radial forearm flaps were harvested.Methods and techniques used during the operation,instant patency rate after anastomosis,the overall survival rate of free flaps,and post-operative complications were recorded.Results Ninety-one anastomoses were performed between 87 pairs of vessels in 37 patients.Fifty recepient vessels were located on ipsilateral side of neck,and the most frequently used recipient vessels were those preserved or not being damaged in former operation and radiation,former transferred free flap vascular pedicles and residual ends of the ligated vessels.Thirty-seven recepient vessels were found on the contralateral side of neck.Thirty-one cases of long-pedicle flap harvesting,2 cases of vein and artery grafting,4 cases of vessel transposition,5 cases of phleboplasties,9 cases of end-to-side anastomoses,and 1 case of flow-through technique were applied in recipient vessels preparation alone or in combination.The patency rate of anastomosis during operation was 100% and the overall survival rate of free flaps was 97.5%.Conclusion Recurrent oral cancer after tumor ablation,second primary oral cancer,osteoradionecrosis and deformity caused by trauma and inflammation are the main reasons of unsuitable recipient vessel conditions in microsurgical reconstruction for oral and maxillofacial defects.Methods including long-pedicle flap harvesting,venous grafting,vessel transposition,phleboplasty,end-to-side anastomosis and flow-through technique applied alone or in combination are still reliable choices for management of neck difficult recipient vessel preparation and anastomosis.
3.Preparation of V(E) acetate-loaded amphiphilic block copolymer nano-dispersions.
Jun LI ; Liandong DENG ; Chunmei YAO ; Yang ZHANG ; Anjie DONG
Journal of Biomedical Engineering 2009;26(1):101-104
V(E) acetate-loaded methoxy poly(ethylene glycol)-b-poly(lactic acid) amphiphilic diblock copolymer nano-dispersion (PMV) was prepared by self-emulsification/solvent evaporation method. The drug-loaded amount, size distribution of PMV nanoparticles, and entrapment efficiency of V(E) acetate (V(E)A) were determined by UV and laser particle analyzer. Drug release in vitro was primarily investigated by UV. The results indicate that the size of PMV nanoparticles is less than 300 nm and PMV is largely influenced by preparation methods, property of solvents, V(E)A-fed amount, and the concentration of dispersion. The initial burst release is not observed and the accumulated release is more than 79% after 14 h. This study develops a new formulation for V(E)A and provides an experimental basis for the novel drug delivery systems of V(E)A.
Delayed-Action Preparations
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chemical synthesis
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Drug Carriers
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administration & dosage
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chemistry
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Hydrophobic and Hydrophilic Interactions
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Nanoparticles
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Polyesters
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administration & dosage
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Polyethylene Glycols
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administration & dosage
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Vitamin E
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administration & dosage
4.Preparation of myocardial ischemia/reperfusion injury model by overlapping line in mice
Zhongshen JIA ; Zhi JIANG ; Anjie LI ; Yueting WU ; Fang WEI
Chongqing Medicine 2017;46(32):4546-4549
Objective To improve the operation speed and reliability of the myocardial ischemia/reperfusion injury(I/R) model in mice.Methods The operative time and the survival rate of the three models were compared.The postoperative leukocyte count and serum IL-6 level were detected to evaluate the inflammatory response.The infarct size was assessed by TTC/Evans blue staining.The left ventricular end diastolic pressure and the pressure change rate were measured by a pressure catheter for the evaluation of cardiac function.Intraditional method,open chest surgery was performed to ligate and loose the left coronary artery(LCA)under the assistance of intubation and ventilation.In novel method,isoflurane was used for anesthesis,and then heart was squeezed out of the chest to ligate and loose LCA with a slipknot.In.overlapping line method,the heart was also squeezed out,but a square knot was performed to ligate LCA and a 2-0 nylon together,and then the nylon thread was drew out to loose LCA.Results Compared with traditional method,the novel method and overlapping line method had significant short operation time(P<0.01 or P<0.05),less white blood cell count and serum IL-6 concentration(P<0.05) and higher mice survival rate(P<0.01);compared with novel method,the overlapping line method further simplified the reperfusion procedure and decreased total procedure time(P<0.01).There was no significant difference in cardiac infarct size and cardiac function by using the three methods(P>0.05),but the overlapping line method had less coefficient of variation compared to novel method.Conclusion Compared with the novel method,the overlapping line method reduces the operation difficulty,shortens the operation time and improves the stability of I/R model.
5.Clinical value of heat shock protein-90α on diagnosis, prediction of treatment response, and monitoring of relapse in breast cancer
Nanlin HU ; Mingzhou LI ; Anjie ZHU ; Jiayu WANG ; Yang LUO ; Fei MA ; Qing LI ; Pin ZHANG ; Binghe XU ; Peng YUAN
Chinese Journal of Clinical Oncology 2018;45(23):1206-1209
To explore the prognostic value of heat shock protein-90α (HSP-90α) plasma levels on breast cancer and non-breast malignant tumors, monitoring the response of chemotherapy, and the predictive value of cancer recurrence and metastasis. Methods: A total of 615 female patients were enrolled between June 2016 and September 2016 in Cancer Hospital, Chinese Academy of Medical Sciences, who were divided into the examination (n=389) and control (n=216) groups. The former group consisted of static (n=289) and dynamic (n=110) groups, which were analyzed by stages, histological and molecular type, and so on. The latter group in-cluded healthy people (n=103), and those with breast benign tumors (n=51) and non-breast malignant tumors (n=62). In all the plasma samples, HSP-90α was detected using a double-antibody enzyme-linked immunosorbent assay. The receiving-operating characteristic curve was used to analyze the effectiveness of plasma HSP-90α in the diagnosis of breast cancer. Wilcoxon's rank test and the Kruskal-Wallis test were used to analyze the association between clinical characteristics and levels of plasma HSP-90α. Results: The levels of plasma HSP-90α were significantly higher in patients with breast cancer than in healthy controls (P<0.001). When the cut-off value was set as 59.7 ng/mL for the diagnosis of breast cancer and 43.22 ng/mL for disease recurrence, the areas under the curve were 0.834 and 0.877, sensitivities were 90.3% and 95.7%, and specificities were 78.6% and 74.5%, respectively. The levels of plasma HSP-90α sig-nificantly decreased after achieving a response to neoadjuvant chemotherapy or surgery (P<0.05). Conclusions: Plasma HSP-90α has good clinical value in the diagnosis and monitoring of response and recurrence in breast cancer.
6.Optimization of the Purification Technology of Total Triterpenoids of Ligustrum lucidum by Box-Behnken Re- sponse Surface Methodology
Shuang GAO ; Yuzhen WANG ; Fumin BO ; Li ZHANG ; Yue SUN ; Shanjing YANG ; Anjie FENG ; Lingjun LI
China Pharmacy 2020;31(18):2196-2201
OBJECTIVE:To i mprove the transfer rate and purity of oleanolic acid and ursolic acid in total triterpenoids from Ligustrum lucidum ,so as to optimize the purification technology. METHODS :Oleanolic acid and ursolic acid were used as representative components of total triterpenoids ,and their contents were determined by HPLC. The determination was performed on Thermo BDS Hypersil C 18 column with mobile phase consisted of methanol- 0.02% ammonium acetate solution (80∶20,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 210 nm,and column temperature was 30 ℃. The sample size was 20 μ L. In single factor tests,using transfer rate of oleanolic acid and ursolic acid as index ,the effects of water precipitation temperature and time ,the amount of redissolved ethanol on the purification technology was investigated ;using transfer rate and purity of two components as indexes ,the effects of the amount of activated carbon and volume fraction of crystallization ethanol were investigated. Based on it ,using the amount of redissolved ethanol and activated carbon ,volume fraction of crystallization ethanol as factors ,Box-Behnken response surface methodology was used to optimize the purification technology ,and validation tests were performed. RESULTS :The optimal purification technology was adding 4-fold(mL/g,the same below )water in L. lucidum concentrated solution ,placing for 2 hours at 0 ℃(water precipitation );adding 1-fold ethanol to dissolve (redissolution); adding 4% activated carbon (edulcoration);finally adding water to adjust the volume fraction of ethanol to 80%,placing at 4 ℃ for 12 hours(crystallization),centrifuging and drying. The results of 3 times of validation tests showed that the transfer rates of oleanolic acid and ursolic acid in total triterpenoids prepared by optimized technology were 61.11% and 65.78%,the purities of them were 53.44% and 19.79%,and RSDs were both lower than 3%. CONCLUSIONS :The optimized purification technology has high extraction efficiency and simple operation ,which can be used for industrial production of purification of total triterpenoids from L. lucidum and the development of corresponding preparations.
7.Microbial lipids production from wastes by Metschnikowia pulcherrima: a review.
Qian LI ; Danyang WANG ; Anjie LI ; Jidong GU
Chinese Journal of Biotechnology 2021;37(8):2753-2764
Biodiesel is an alternative fuel to addressing the energy shortage problem. Microbial lipids have attracted widespread attention as one of the potential feed-stocks for cost-effective and efficient biodiesel production. However, the large-scale production of microbial lipids is hampered by the complexity and the high cost of aseptic culturing approach. Metschnikowia pulcherrima is an oleaginous yeast with strong environmental adaptability. It is capable of utilizing a wide spectrum of substrates, and can be cultured under non-sterile conditions. Therefore, this yeast has great potential to replace the traditional oleaginous microorganisms, particularly in the area of recycling wastewater and solid waste for the production of biodiesel. Based on the analysis of lipid production and application conditions of M. pulcherrima, this review summarized the unique advantages of M. pulcherrima and the key factors affecting lipids production. We further discussed the feasibility of cultivating M. pulcherrima on various organic wastes under non-sterile conditions for lipids production. Moreover, we analyzed the challenges associated with M. pulcherrima's in the yield and mechanism for lipids production, and proposed perspectives for how to achieve efficient biodiesel production using this yeast.
Biofuels
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Candida
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Lipids
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Metschnikowia
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Yeasts