During a period from February 1st to November 30th in 1983, measurements were made twice a month on dustfalls and concentrations of various cations and anions in their filtrates in an urban (Heuksuk-dong, Seoul) and in a rural (Ansung) districts. Standard British Deposit Gauge method was applied for collection of dustfalls and ion chromatographic method using Ion Chromatograph 10 (Dionex, U.S.A.) for determination of cation and anion concentrations. The results obtained were as follows: 1. Annual mean values of dustfalls were 8.30+/-5.09 tons/km2/month in an urban and 6.20+/-0.82 tons/km2/month in a rural districts. And annual mean values of pH of filtrates of the collected samples were 5.3+/-0.76 in an urban and 6.0+/-0.82 in a rural districts. 2. Annual mean concentrations of common cations and anions in their filtrates were as follows: Some of anions such as Br-, PO4-3 and NO2 were scarcely detected and F was measured at very low concentrations ranging from 0.1 to 0.5 ppm in Feb., Oct. and Nov. in an urban and in Feb. in a rural districts. 3. By two-way analysis of variance with 4 replications for dustfalls, pH and concentrations of various ion by district and month of a year, statistically significant differences were noted in dustfalls (p<0.01), pH (p<0.01) and concentrations of various ion (p<0.01) by month of a year, and in dustfalls (p<0.05) and concentrations of various ion such as NH4, Ca, NO3 and SO4 (p<0.01) by district. 4. There were statistically significant differences pH in Spring (Feb., Mar. and Apr.) (p<0.01) and in SO4 in Autumn (Aug., Sept., Oct. and Nov.) (p<0.05) between the two districts.
Anions ; Cations ; Hydrogen-Ion Concentration

The anion gap in the serum is useful in the interpretation of acid-base disorders and in the diagnosis of other conditions. In the early 1980s, ion-selective electrodes for specific ionic species were introduced for the measurement of serum electrolytes. This new method has caused a shift of the anion gap from 12+/-4 mEq/L down 6+/-3 mEq/L. It is worthy for clinicians to understand the range of normal anion gap and the measuring methods for serum sodium and chloride in the laboratories that support their practice. While an increase in the anion gap is almost always caused by retained unmeasured anions, a decrease in the anion gap can be generated by multiple mechanisms.
Acid-Base Equilibrium* ; Anions ; Diagnosis ; Electrolytes ; Ion-Selective Electrodes ; Sodium

Air ; analysis ; Anions ; analysis ; Chromatography, Ion Exchange ; Ions ; analysis ; Workplace

The cerebrospinal fluid(CSF) contains only negligible concentrations of buffer anions other than HCO3- because there is no hemoglobin buffer syatem. CSF gas values have been reported and it has been shown that arterial CO2 diffuses easily into the blood brain barrier to form H2CO3 in the CSF. No study for normal valuea of CSF gaaes in Koreans has been reported. The study was attempted to obtain the normal. values of CSF gases and also to compare values of gases in the three different components, j,e. CSF, arterial and venous blood. Relatively healthy patients with no respiratory or systemic disturbances who were sch-eduled for simple operations have been studied. Prior to the induction of aneathesia, Ium-bar tapping was Performed at a level of L3-4 using a 22 gauge needle and CSF samples-were obtained. Then the racial samples were taken. The subclavian vein was cannulated with a 20 gauge catheter and venous b1ood samples were obtained. All the gas values were-compared as shown in the tab1es and figures. The results obtained were as fallows: PCSF O2 was 76.5+/-8.6 torr and PcsF CO2 was 44.9+/-3.6 torr. Both data in the CSF fall in the middle of the arterial and venous valses. The pH of the CSF was 7.329+/-0.017, HCO3- was 23.6+/-1.8 mEq/L and the base excess was -2.2+/-1.5. All the above data are the lowest among the three components, The pH of the CSF is more acid than arterial or venous blood and that pH regulation of the CSF is seemed to be limited because there is no compensatory hemoglobin buffer in the CSF. The finding of the CSF gas values obtained in this study is introduced as a normal data for further physiological study of the CSF.
Anions ; Blood-Brain Barrier ; Catheters ; Gases ; Humans ; Hydrogen-Ion Concentration ; Needles ; Subclavian Vein

BACKGROUND: Diabetes mellitus is the most common endocrinopathy encountered in the perioperative period and has long been assumed to increase perioperative risk. However, when diabetes mellitus was segregated from old age and the complications of it, it was questioned that diabetes mellitus itself increased perioperative risk. In this study, we investigated the influence of hyperglycemia on the length of postoperative hospital stay. METHODS: We studied 100 patients undergone intra-abdominal operations with general anesthesia. These patients were divided into the hyperglycemic group (n = 20) with postoperative blood glucose level higher than 10 mM and the non-hyperglycemic group (n = 80) with glucose level lower than 10 mM and we investigated the length of postoperative hospital stay, serum electrolyte, serum chemistry, arterial blood gas values, and base excess by unmeasured anions. We also divided these patients into the diabetic patients group (n = 15) and the non-diabetic patients group (n = 85) and compared the same variables. RESULTS: The length of postoperative hospital stay was significantly prolonged in the hyperglycemic group (20.9 +/- 9.0 days) compared with the non-hyperglycemic group (16.2 +/-8.5 days), and the cumulative postoperative hospital stay curves based on Kaplan-Meier method also showed significant difference between the two groups. When we compared the length of postoperative hospital stay between the diabetic and the non-diabetic patients, there was no significant difference. CONCLUSIONS: This study demonstrated that hyperglycemia prolonged the length of postoperative hospital stay. This finding suggests that the patient's glucose level should be monitored and controlled within an adequate range perioperatively.
Anesthesia, General ; Anions ; Blood Glucose ; Chemistry ; Diabetes Mellitus ; Glucose ; Humans ; Hyperglycemia* ; Length of Stay* ; Perioperative Period

OBJECTIVETo investigate the effects of sub-micro emulsion composition on cellular uptake and disposition of incorporated lipophilic drug.

METHODSSub-micro emulsions containing 10 % oil, 1.2 % lecithin and 2.25 % glycerol were prepared, and the fluorescent agent coumarin 6 was used as a model drug. The effects of oil types, co-surfactants and cationic lipid on uptake and elimination kinetics of 6-coumarin in HeLa cells were studied. The uptake mechanism of sub-micro emulsions was further investigated.

RESULTSOil type and Tweens had no influence on the cellular uptake. Modifications of surfactants with Span series increased the cellular influx, among which Span 20 with hydrophilic-lipophilic balance (HLB) value of 8.6 was the best enhancer. The intracellular drug level reached up to (46.09 ± 1.98)ng/μg protein which had significant difference with control group [(38.54 ± 0.34)ng/μg protein]. The positively charged emulsions significantly increased the uptake rate constant and elimination rate constant which were 4 times and 1.5 times of those in anionic groups, respectively. The uptake enhancement was also observed in cationic emulsions, cellular concentrations at plateau were (42.73 ± 0.84)ng/μg protein, which was about 3 times of that in anionic emulsions [(15.71 ± 0.74)ng/μg protein], when extracellular drug concentration kept at 100 ng/ml. Cationic emulsions delivered the payload mainly by direct drug transfer to contacted cells, while the negative ones depended on both drug passive diffusion and clathrin-mediated endocytosis of drug containing oil droplets which accounted for 20% of the intracellular drug.

CONCLUSIONInterfacial characteristic of sub-micro emulsions such as co-surfactants HLB as well as zeta potentials can influence lipophilic drug both in cellular uptake and elimination.

Anions ; Cations ; Coumarins ; pharmacokinetics ; Emulsions ; Endocytosis ; HeLa Cells ; Humans ; Surface-Active Agents ; pharmacokinetics ; Thiazoles ; pharmacokinetics

OBJECTIVETo study the characterization apportionment of main ion concentrations of PM10 under the influence of trajectory wind direction in London.

METHODSPM10 samples from 1 May 1995 to 30 October 1995 of Oxford Street of Central London were collected, the metals and anions of which were measured using atomic absorption spectrometry (AAS) and ion chromatography (IC). Composite trajectories representative of the air mass arriving in London at the same period were calculated based on basic routine back trajectories from the British Atmospheric Data Centre (BADC).

RESULTSConcentration apportionments of main ions were similar when the trajectory was plotted back at 6 h, 12 h, and 24 h, some were obviously different. Mg, Ba, Pb, and Cu had similar peak apportionments at the area 180 degrees-320 degrees, but Zn and Ni at the area of 90 degrees-270 degrees, NO3 and SO4(2-) at the area of 100 degrees-220 degrees. Cl- concentration peak apportionment was at the area of 220 degrees-300 degrees, which showed that Cl- mainly came from the North Sea.

CONCLUSIONTrajectory wind direction has important effect on ion concentration apportionment of PM10 in London. The ions have similar concentration peak apportionments or their correlation coefficients are statistically significant.

Air Pollutants ; analysis ; chemistry ; Anions ; analysis ; Cations ; analysis ; Environmental Monitoring ; London ; Particle Size ; Time Factors ; Wind

Metabolic acidosis is the most frequent acid-base disorder in critically ill patients and dialysis patients. This study is to compare the conventional approach with the physicochemical approach between the intensive care unit (ICU) and hemodialysis (HD) patients. Fifty-seven ICU patients and 33 HD patients were enrolled. All data sets included simultaneous measurements of arterial blood gas with base deficit (BD), serum electrolytes, albumin, lactate, and calculated anion gap observed (AGobs). Physiochemical analysis was used to calculate the albumin and lactate-corrected anion gap (AGcorr), the base deficit corrected for unmeasured anions (BDua), the strong ion difference apparent (SIDa), the strong ion difference effective (SIDe), and the strong ion gap (SIG). The SIDa (37.5+/-5.3 vs 33.9+/-9.0, p=0.045) and SIG (12.3+/-5.3 vs 8.6+/-8.8, p=0.043) was significantly higher in the HD group than the ICU group. SIG in the ICU group showed the highest correlation coefficient with AGobs, whereas SIG in the HD group with AGcorr. Concerning the contributions of the three main causes of metabolic acidosis, increased SIG was comparable between the ICU and HD group (n=48, 90.6% vs n=30, 93.8%), whereas hyperlactatemia (n=9, 17.0% vs n=0, 0%) and hyperchloremia (n=20, 35.1% vs n=2, 6.1%) was significantly increased in the ICU group compared with the HD group. Multiple underlying mechanisms are present in most of the ICU patients with metabolic acidosis compared with the HD patients. In conclusion, the physicochemical approach can elucidate the detailed mechanisms of metabolic acidosis in ICU and HD patients compared with conventional measures.
Acid-Base Equilibrium ; Acidosis* ; Anions ; Critical Illness ; Dataset ; Dialysis ; Electrolytes ; Humans ; Intensive Care Units ; Lactic Acid ; Renal Dialysis*

Plasmid DNA (pDNA) is used as an important vector for gene therapy, and its wide application is restricted by the purity and yield. To obtain high-purity pDNA, a chromatographic method based on anion-exchange supermacroporous cryogel was explored. The anion-exchange cryogel was prepared by grafting diethylaminoethyl-dextran to the epoxide groups of polyacrylamide-based matrix and pUC19 plasmid was used as a target to test the method. The plasmid was transferred into Escherichia coli DH5alpha, cultivated, harvested and lysed. The obtained culture was centrifuged and the supernatant was used as the plasmid feedstock, which was loaded into the anion-exchange cryogel bed for chromatographic separation. By optimizing the pH of running buffer and the elution conditions, high-purity pDNA was obtained by elution with 0.5 mol/L sodium chloride solution at pH 6.6. Compared to the traditional methods for purification of pDNA, animal source enzymes and toxic reagents were not involved in the present separation process, ensuring the safety of both the purification operations and the obtained pDNA.
Anions ; Chromatography, Ion Exchange ; methods ; Cryogels ; chemical synthesis ; DNA ; isolation & purification ; Genetic Vectors ; isolation & purification ; Plasmids ; isolation & purification ; Porosity

Cytolysin produced by Vibrio vulnificus has been incriminated as one of the important virulence determinants in V. vulnificus infection. Ion selectivity of cytolysin-induced pores was examined in a CPAE cell, a cell line of pulmonary endothelial cell, using inside-out patch clamp techniques. In symmetrical NaCl concentration (140 mM), intracellular or extracellular application of cytolysin formed ion-permeable pores with a single channel conductance of 37.5 4.0 pS. The pore currents were consistently maintained after washout of cytolysin. Replacement of Na in bath solution with monovalent ions (K, Cs or TEA ) or with divalent ions (Mg2, Ca2 ) did not affect the pore currents. When the NaCl concentration in bath solution was lowered from 140 to 60 and 20 mM, the reversal potential shifted from 0 to 11.8 and 28.2 mV, respectively. The relative permeability of the cytolysin pores to anions measured at 40 mV was Cl = NO2 > or = Br = I > SCN > acetate > isethionate > ascorbic acid > EDTA2, in descending order. The cytolysin-induced pore current was blocked by Cl channel blockers or nucleotides. These results indicate that V. vulnificus cytolysin forms anion-selective pores in CPAE cells.
Anions ; Ascorbic Acid ; Baths ; Cell Line ; Endothelial Cells* ; Ions ; Nucleotides ; Patch-Clamp Techniques ; Perforin* ; Permeability ; Tea ; Vibrio vulnificus* ; Vibrio* ; Virulence